Skip to main content
Addgene
Showing: 3651 - 3700 of 157134 results

  3. OPRK1-Tango

    Plasmid
    #66462
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRK1 (OPRK1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66462-plasmid-map-sequence-id-157905

    Enlarge

  16. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

    Enlarge

  17. TFORF2954

    Plasmid
    #144430
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4G (HNF4G Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144430-plasmid-map-sequence-id-392354

    Enlarge

  21. pALS2-sfGFP WT

    Plasmid
    #197575
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197575-plasmid-map-sequence-id-396574

    Enlarge

  24. pMSCVhyg-DEST

    Plasmid
    #119747
    Purpose
    Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Hygromycin resistance)
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119747-plasmid-map-sequence-id-233679

    Enlarge

  28. psfTn5

    Plasmid
    #79107
    Purpose
    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations
    Depositor
    Sten Linnarsson
    Insert
    transposase 5
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    E54K and L372P
    Promoter
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    79107-plasmid-map-sequence-id-418885

    Enlarge

  29. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211448-plasmid-map-sequence-id-419419

    Enlarge

  30. mEGFP-pBAD

    Plasmid
    #54622
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis and mEGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54622-plasmid-map-sequence-id-257491

    Enlarge

  31. MR015-H11-L2-TALEN

    Plasmid
    #51554
    Purpose
    Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-L2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51554-plasmid-map-sequence-id-78782

    Enlarge

  34. MR015-H11-R2-TALEN

    Plasmid
    #51555
    Purpose
    Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-R2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51555-plasmid-map-sequence-id-78783

    Enlarge

  37. pDEST14-DoubleCatcher-H-Lock

    Plasmid
    #216285
    Purpose
    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    DoubleCatcher H-Lock
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216285-plasmid-map-sequence-id-433331

    Enlarge

  44. pM2s2AsG

    Plasmid
    #137924
    Purpose
    E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    Superfolder GFP
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    137924-plasmid-map-sequence-id-331367

    Enlarge

  47. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

    Enlarge

  50. EF1a_Puro_Telo_v2

    Plasmid
    #195139
    Purpose
    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction
    Depositor
    Jason Sheltzer
    Article
    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11
    Insert
    Telomere (RTEL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195139-plasmid-map-sequence-id-442720

    Enlarge

Showing: 3651 - 3700 of 157134 results