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Showing: 3651 - 3700 of 169365 results

  12. P362-FBP_12

    Plasmid
    #139703
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139703-plasmid-map-sequence-id-296521

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  14. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Expression
    Mammalian
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

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  15. pOpen-BstpolLF

    Plasmid
    #165553
    Purpose
    Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165553-plasmid-map-sequence-id-339898

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  17. MT1G

    Plasmid
    #105704
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1g
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1g (MT1G Human)
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105704-plasmid-map-sequence-id-203324

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  19. mApple-hCOL1A1

    Plasmid
    #140113
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140113-plasmid-map-sequence-id-296772

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  22. TFORF1727

    Plasmid
    #142837
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF692 (ZNF692 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142837-plasmid-map-sequence-id-462906

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  26. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

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  30. pcDNA3-Flag-Hsp40-S171A

    Plasmid
    #84999
    Purpose
    Hsp40/DnaJB1 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp40 (DNAJB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 171 mutated to Alanine
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. WARS_pNIC-CTB10H

    Plasmid
    #153058
    Purpose
    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    WARS (WARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153058-plasmid-map-sequence-id-304478

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  35. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available Since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  36. RHPMP139 (pLifeAct mEOS3.1)

    Plasmid
    #88839
    Purpose
    Localization by Super-Resolution microscopy of lifeAct fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    LifeAct
    Tags
    mEOS3.1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88839-plasmid-map-sequence-id-177489

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  37. pET28a_SYK

    Plasmid
    #224240
    Purpose
    Expression of human SYK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224240-plasmid-map-sequence-id-459259

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  38. pET28a_LYN

    Plasmid
    #224238
    Purpose
    Expression of human LYN Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224238-plasmid-map-sequence-id-458419

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  40. mCerulean-hCOL1A1

    Plasmid
    #140112
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140112-plasmid-map-sequence-id-296778

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  45. CHRM3-DuET

    Plasmid
    #213209
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM3 (CHRM3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213209-plasmid-map-sequence-id-430771

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  50. TFORF2442

    Plasmid
    #142080
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ABL1 (ABL1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142080-plasmid-map-sequence-id-395698

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Showing: 3651 - 3700 of 169365 results