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179,463 results

Showing: 701 - 750 of 179463 results

  2. pGP-AAV-syn-jGCaMP8f-WPRE (AAV1)

    Viral prep
    #162376-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid
    #44362
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Promoter
    human Synapsin 1
    Available since
    April 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    437 Articles
    44362-plasmid-map-sequence-id-292479

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  12. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    138147-plasmid-map-sequence-id-349848

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  13. pAAV-CAG-tdTomato (codon diversified) (AAV1)

    Viral prep
    #59462-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.eB)

    Viral prep
    #59462-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral prep
    #59171-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Publication
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    44720-plasmid-map-sequence-id-179979

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  21. pBABE puro K-Ras V12

    Plasmid
    #9052
    Purpose
    Retroviral expression of a constitutively active form of K-Ras
    Depositor
    William Hahn
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    G12V. Constitutively active.
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    9052-plasmid-map-sequence-id-219889

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  22. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV9)

    Viral prep
    #20297-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  26. mCherry-ER-3

    Plasmid
    #55041
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58 Articles
    55041-plasmid-map-sequence-id-173833

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  28. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    92308-plasmid-map-sequence-id-216421

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  29. AAV.rTH.PI.Cre.SV40 (AAV9)

    Viral prep
    #107788-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    rTH
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pcDNA-dCas9-VP64

    Plasmid
    #47107
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    dCas9-VP64
    Use
    CRISPR
    Tags
    Flag, HA, SV40 NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    47107-plasmid-map-sequence-id-398036

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  35. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid
    #91793
    Purpose
    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Insert
    Puro-P2A-2xHA-FKBP_F36V
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Phenylalanine 36 to valine in FKBP12
    Promoter
    Promotorless
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    91793-plasmid-map-sequence-id-342048

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  36. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV5)

    Viral prep
    #26966-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pAAV-EF1a-Flpo (AAV1)

    Viral prep
    #55637-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-Flpo (#55637). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flpo plasmid DNA. EF1a-driven Flpo recombinase expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-hSyn-EGFP (AAV1)

    Viral prep
    #50465-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  49. pAAV-hSyn-mCherry (AAV5)

    Viral prep
    #114472-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only

  50. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Publication
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    34 Articles
    63142-plasmid-map-sequence-id-339931

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Showing: 701 - 750 of 179463 results