Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

177,971 results

Showing: 701 - 750 of 177971 results

  1. pAAV-hSyn-DIO-hM4D(Gi)-mCherry

    Plasmid
    #44362
    Purpose
    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Promoter
    human Synapsin 1
    Available Since
    April 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    44362-plasmid-map-sequence-id-292479

    Enlarge

  5. pAAV-hsyn-GRAB_DA2m (AAV9)

    Viral Prep
    #140553-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2m (#140553). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2m plasmid DNA. Synapsin-driven expression of 2nd generation medium affinity dopamine sensor GRAB_rDA2m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-hSyn-hM3D(Gq)-mCherry (AAV2)

    Viral Prep
    #50474-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV-hSyn-mCherry (AAV9)

    Viral Prep
    #114472-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  11. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

    Enlarge

  12. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available Since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

    Enlarge

  14. pBABE puro K-Ras V12

    Plasmid
    #9052
    Purpose
    Retroviral expression of a constitutively active form of K-Ras
    Depositor
    William Hahn
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    G12V. Constitutively active.
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    9052-plasmid-map-sequence-id-219889

    Enlarge

  15. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  16. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (AAV9)

    Viral Prep
    #100842-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 (#100842). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available Since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV-CAG-tdTomato (codon diversified) (AAV1)

    Viral Prep
    #59462-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

    Enlarge

  25. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep
    #20297-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  26. mCherry-ER-3

    Plasmid
    #55041
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55041-plasmid-map-sequence-id-173833

    Enlarge

  28. pAAV-CAG-tdTomato (codon diversified) (AAV PHP.eB)

    Viral Prep
    #59462-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep
    #59171-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. AAV.rTH.PI.Cre.SV40 (AAV9)

    Viral Prep
    #107788-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV.rTH.PI.Cre.SV40 (#107788). In addition to the viral particles, you will also receive purified AAV.rTH.PI.Cre.SV40 plasmid DNA. rTH promoter driving Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    rTH
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV5)

    Viral Prep
    #26966-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

    Enlarge

  48. pCW57-MCS1-2A-MCS2

    Plasmid
    #71782
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Available Since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71782-plasmid-map-sequence-id-222919

    Enlarge

Showing: 701 - 750 of 177971 results