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20,806 results

Showing: 9501 - 9550 of 20806 results

  12. pS-cr:GFP

    Plasmid
    #196295
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Tags
    SpeI-DR-BsaI-DR-SpeI
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196295-plasmid-map-sequence-id-390035

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  17. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

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  18. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available Since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  22. Hs.KRAS4b G12D

    Plasmid
    #83131
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83131-plasmid-map-sequence-id-158094

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  34. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  42. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  46. pTiger

    Plasmid
    #1728
    Purpose
    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system
    Depositor
    Garry Nolan
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Aug. 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    1728-plasmid-map-sequence-id-105963

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  50. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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Showing: 9501 - 9550 of 20806 results