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20,801 results

Showing: 2751 - 2800 of 20801 results

  1. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  6. pET-scFv-T

    Plasmid
    #67843
    Purpose
    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
    Depositor
    Joanna Bereta
    Article
    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
    Insert
    antibody 1D8 light kappa chain
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67843-plasmid-map-sequence-id-117725

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  16. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100848-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pCW57.1 CMYC

    Plasmid
    #164145
    Purpose
    Doxycycline inducible expression of c-MYC
    Depositor
    Richard Possemato
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Doxycycline inducible promoter
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    164145-plasmid-map-sequence-id-325859

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  28. pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

    Viral Prep
    #137127-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available Since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  30. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available Since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  31. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)

    Viral Prep
    #104491-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51005-plasmid-map-sequence-id-490664

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  36. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  39. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Bacterial
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  41. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  44. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  45. pAAV-Ef1a-fDIO-GCaMP6f (AAV1)

    Viral Prep
    #128315-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-GCaMP6f (#128315). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-GCaMP6f plasmid DNA. EF1a-driven, Flp-dependent expression of calcium sensor GCaMP6f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Patricia Jensen
    Article
    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi
    Promoter
    EF1a
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2751 - 2800 of 20801 results