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179,463 results

Showing: 251 - 300 of 179463 results

  2. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Publication
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Expression
    Bacterial
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    89566-plasmid-map-sequence-id-177206

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  6. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral prep
    #50459-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV Retrograde)

    Viral prep
    #44361-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    96 Articles
    12257-plasmid-map-sequence-id-237089

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  17. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50 Articles
    54563-plasmid-map-sequence-id-310151

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  18. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    94 Articles
    20672-plasmid-map-sequence-id-203241

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  20. pAAV-FLEX-tdTomato (AAV9)

    Viral prep
    #28306-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  22. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Insert
    Clover GFP
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40 Articles
    40259-plasmid-map-sequence-id-215680

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  25. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Expression
    Mammalian
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    253 Articles
    22036-plasmid-map-sequence-id-339901

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  26. pAAV-EF1a-Cre (AAV Retrograde)

    Viral prep
    #55636-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available since
    Nov. 12, 2020
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-DIO-EGFP (AAV Retrograde)

    Viral prep
    #50457-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  28. TALE Toolbox

    Plasmid kit
    #1000000019
    Purpose
    A complete set of plasmids, optimized for human expression, to build your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Application
    Genome Editing
    Vector type
    Mammalian Expression
    Editing type
    TALEN
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  29. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Publication
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68 Articles
    86708-plasmid-map-sequence-id-317051

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  32. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    57 Articles
    13425-plasmid-map-sequence-id-360003

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  35. pAAV-FLEX-tdTomato (AAV Retrograde)

    Viral prep
    #28306-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. Cre-dependent (FLEX) tdTomato expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (Cre-dependent)
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV-CAG-GFP

    Plasmid
    #37825
    Purpose
    AAV-mediated expression of GFP under the CAG promoter
    Depositor
    Edward Boyden
    Has service
    AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV9-X1.1, AAV11, AAV11 trial size, AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size
    Insert
    GFP
    Use
    AAV; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    169 Articles
    37825-plasmid-map-sequence-id-448204

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  42. Lentiviral-TOP-dGFP-reporter

    Plasmid
    #14715
    Purpose
    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).
    Depositor
    Tannishtha Reya
    Publication
    Reya et al Nature. 2003 May 22. 423(6938):409-14.
    Insert
    LEF-1/TCF-responsive promoter (Ctnnb1 )
    Use
    Lentiviral
    Tags
    d2 eGFP
    Expression
    Mammalian
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    14715-plasmid-map-sequence-id-339924

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  46. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV9)

    Viral prep
    #20298-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 251 - 300 of 179463 results