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Showing: 101 - 150 of 74807 results

  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  6. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid
    #169247
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169247-plasmid-map-sequence-id-343245

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  9. pAS_4xMma PylT_FLAG-AbKRS-chIPYE

    Plasmid
    #140020
    Purpose
    Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…
    Promoter
    EF1
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140020-plasmid-map-sequence-id-297657

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  10. pcDNA3-ExRai-AktAR2

    Plasmid
    #184047
    Purpose
    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.
    Depositor
    Jin Zhang
    Article
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Insert
    ExRai-AktAR2
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184047-plasmid-map-sequence-id-363388

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  14. p663-UBC-miniTurbo-V5-PRKACB_IDG-K

    Plasmid
    #170602
    Purpose
    Gateway destination clone of PRKACB (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PRKACB (PRKACB Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170602-plasmid-map-sequence-id-335769

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  18. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

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  25. L1-neo-TET

    Plasmid
    #51284
    Purpose
    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    L1RE1 (L1RE1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51284-plasmid-map-sequence-id-78366

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  26. pTriEx4-Cdc42-2G

    Plasmid
    #68814
    Purpose
    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
    Depositor
    Olivier Pertz
    Article
    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
    Insert
    Cdc42 activation reporter
    Use
    Tags
    His and Myc
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    68814-plasmid-map-sequence-id-120972

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  43. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  48. pXJ42-p200 CUX1

    Plasmid
    #100813
    Purpose
    Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag
    Depositor
    Alain Nepveu
    Article
    Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do
    Insert
    p200 CUX1 (CUX1 Human)
    Use
    Note that this plasmid does not have an sv40 ori
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    100813-plasmid-map-sequence-id-205855

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  49. pCdc45-mCherry

    Plasmid
    #211380
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mCherry
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211380-plasmid-map-sequence-id-420495

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Showing: 101 - 150 of 74807 results