165,343 results

1. mCherry-VAB(VPS13C)

   Plasmid

   #232865

   Purpose

   Expresses the VAB domain of VPS13C in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

   Insert

   the VAB domain of VPS13C (VPS13C Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Plasmid

   #159540

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   G12C

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTXTL-T7max-RLuc

   Plasmid

   #188312

   Purpose

   Expression of Renilla luciferase via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   Renilla luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG-Hc∆18-AA-IL-13

   Plasmid

   #86171

   Purpose

   Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

   Depositor

   Jakob Reiser

   Article

   Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

   Insert

   Measles virus H glycoprotein fused to IL-13
   

   Tags

   6x His

   Expression

   Mammalian

   Mutation

   Y463A, R515A

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGC-1 alpha promoter luciferase delta MEF

   Plasmid

   #8889

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dMEF (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the MEF2 bind…

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-PLCd1-mNGx1

   Plasmid

   #202751

   Purpose

   expresses fluorescent protein-tagged lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   PLCD1v2(1-170):mNG2 (PLCD1 Human)
   

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_PAK1_WT

   Plasmid

   #82220

   Purpose

   Gateway Donor vector containing PAK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PAK1 (PAK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTEI039

   Plasmid

   #105428

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   LexA-VP16-ER (transcription factor: inducible expression; estradiol)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. HIS-HA-CMYC P53

   Plasmid

   #233738

   Purpose

   expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

   Depositor

   Albert Keung

   Article

   Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

   Insert

   HIS-HA-CMYC-p53 (TP53 Human)
   

   Tags

   HIS-HA-CMYC

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFlag-tdMCP-Erk2 (pErk reporter)

    Plasmid

    #226167

    Purpose

    Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-Erk2 (MAPK1 Human)
    

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ-mgU2-47-Mango II

    Plasmid

    #127586

    Purpose

    Expresses mgU2-47-Mango II scaRNA from a mU6 promoter

    Depositor

    David Rueda

    Article

    Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

    Insert

    mgU2-47 scaRNA with Mango II tag
    

    Expression

    Mammalian

    Promoter

    modified U6

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. F-MED26

    Plasmid

    #15367

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED26 (MED26 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. EC2_10_dCas9_VPR_HC_sgRNA

    Plasmid

    #163712

    Purpose

    Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CamkII-tTA-2A-E2-Crimson-WPRE-pA

    Plasmid

    #183708

    Purpose

    AAV designed to express tTA and-E2 Crimson from a CamKIIa promoter

    Depositor

    Jonathan Ploski

    Insert

    tTA
    

    Use

    AAV

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RMCE ABCDE>ala DNA-PKcs

    Plasmid

    #233914

    Purpose

    Low copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs ABCDE phosphorylation site mutant (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    phosphorylation sites 2609, 2612, 2620, 2624, 263…

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-mCh-kCAAX

    Plasmid

    #112267

    Purpose

    ITRACK Acceptor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    mCherry with KRas membrane targeting domain
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid

    #50468

    Purpose

    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Insert

    rM3D-IRES-mCitrine
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Bovine, Mouse)
    

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Promoter

    T7 polymerase

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

    Plasmid

    #180587

    Purpose

    FdCd

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Cre
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. V309 - pDNR MCS SA lacZ alpha - Donor

    Plasmid

    #11692

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pLVUTHshGATA1-tTR-KRAB

    Plasmid

    #11650

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZA69

    Plasmid

    #174782

    Purpose

    RNAi

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    Intron-containing hairpin RNA (ihpRNA)-NbZAR1
    

    Use

    RNAi

    Mutation

    WT

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD649-HAsp-KIR2DL5A-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156643

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL5A (KIR2DL5A Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP13450: pAAV-AiE0140h-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN3450)

    Plasmid

    #214845

    Purpose

    Expression of iCre in striatal Pvalb neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    iCre
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cacna2d1 HA pCAGGS

    Plasmid

    #206107

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

    Depositor

    Annette Dolphin

    Article

    Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV/B-actin

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHLSec2-irisin-his

    Plasmid

    #122729

    Purpose

    Expresses in mammalian cells (HEK293):glycosylated and secreted irisin-his

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Bovine, Mouse)
    

    Tags

    his and signal sequence for secretion

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

    Plasmid

    #168305

    Purpose

    mediated knock-in of sgRNA precursor

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    SaKKH-EGFP-U6-sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1alpha, CBh

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RabV CVS-N2c(deltaG)-mCherry-P2A-Cre

    Plasmid

    #73472

    Purpose

    Expresses mCherry and Cre for monosynaptic circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    mCherry-P2A-Cre
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBiT3.1-N_RBBP7:1-425

    Plasmid

    #211113

    Purpose

    Mammalian expression of full-length human RBBP7. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RBBP7:M1-S425 (RBBP7 Human)
    

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS316-4M5.3

    Plasmid

    #45831

    Depositor

    Dane Wittrup

    Insert

    4M5.3
    

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCK033 p5E desma MCS

    Plasmid

    #195948

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    desmin a regulatory elements (desma Zebrafish)
    

    Use

    Other

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLKO.1 puro shSLC2A1-1

    Plasmid

    #193702

    Purpose

    Constitutive lentiviral expression of SLC2A1 shRNA

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    SLC2A1 (SLC2A1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR P2R-P3-mKate2

    Plasmid

    #48345

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Mutation

    Contains a stop codon at the 3' end of the c…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BS II KS+ loxP-GFP_ZEO_loxP

    Plasmid

    #128064

    Purpose

    has loxp-GFP-Zeo-Loxp cassette

    Depositor

    Oskar Laur

    Insert

    loxp-GFP-Zeo-Loxp
    

    Use

    Cre/Lox

    Mutation

    no

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDONR P2R-P3-EGFP

    Plasmid

    #48349

    Purpose

    Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Mutation

    Aminoacid 40 is valine in our sequence, while it …

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GGE0029

    Plasmid

    #120730

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCURE

    Plasmid

    #23013

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    I-CreI homing endonuclease
    

    Expression

    Bacterial

    Mutation

    I-CreI expression is controlled by the arabinose …

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDESTmycQKI

    Plasmid

    #19870

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    quaking homolog, KH domain RNA binding (QKI Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only