161,242 results

1. pHAGE Flag HA ADAR1 p110

   Plasmid

   #237854

   Purpose

   Lentiviral vector expressing ADAR1 p110

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   ADAR1 (ADAR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLIK-Rbfox2-rABE-Flag

   Plasmid

   #191384

   Purpose

   Doxycycline inducible expression of Rbfox2-rABE

   Depositor

   Stephen Floor

   Article

   Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

   Insert

   Rbfox2-rABE (RBFOX2 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   tet-inducible promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ALCAM-p2A-E2Crimson

   Plasmid

   #83939

   Purpose

   Lentiviral vector expressing ALCAM-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   ALCAM (ALCAM Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTurquoise2-Paxillin

   Plasmid

   #176107

   Purpose

   Focal Adhesion marker

   Depositor

   Dorus Gadella

   Article

   Arts et al Front Immunol. 2021 May 18;12:667213. do

   Insert

   Paxillin (PXN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AiP12839 - pAAV-AiE0589m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2839)

   Plasmid

   #230136

   Purpose

   AiE0589m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Membrane mCherry

   Plasmid

   #210470

   Purpose

   Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane mCherry (LCK Synthetic, Human, A.victoria)
   

   Use

   Synthetic Biology; Sea urchin, mammal, zebrafish

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MBD2a pcDNA3.1

   Plasmid

   #78141

   Purpose

   Mammalian expression of MBD2a

   Depositor

   Heidi Schwarzenbach

   Article

   Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

   Insert

   MBD2a (MBD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CIBN-GFP-Sec61B

   Plasmid

   #104177

   Purpose

   Expresses fusion of CIB1 (1-170) with GFP and Sec61B

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CIBN-GFP-Sec61B (CIB1, SEC61B Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDM069

    Plasmid

    #216821

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Use

    Tags

    RIPGLIN and VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRL055 pDESTattB_cryaa:Venus

    Plasmid

    #213509

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRY2low-tdTomato

    Plasmid

    #104067

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GB1-A11(2-196)-Strep

    Plasmid

    #196208

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LsgRNA-MS2

    Plasmid

    #235597

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE HA Chk2 WT

    Plasmid

    #41901

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    CHK2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DepoPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-RLuc8.6-535

    Plasmid

    #87125

    Purpose

    Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid

    #178185

    Purpose

    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.

    Depositor

    Irmela Jeremias

    Article

    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:

    Inserts

    GLuc
    CreERT2
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIP sHRPa-Aga1P

    Plasmid

    #73151

    Purpose

    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-Aga1P
    

    Use

    Tags

    V5 tag

    Expression

    Yeast

    Mutation

    Promoter

    glyceraldehyde-3-phosphate dehydrogenase GPD prom…

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHT-ads

    Plasmid

    #47382

    Purpose

    Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-NRCH-PE2max-BSD

    Plasmid

    #191103

    Purpose

    Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    NRCH-PEmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9-NRCH

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-RET-V5/HIS

    Plasmid

    #205606

    Purpose

    Expression of human RET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RET receptor tyrosine kinase (RET Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GB1-A11(2-88)-Strep

    Plasmid

    #196214

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBI-MCS-EYFP

    Plasmid

    #58855

    Purpose

    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAD-IRP

    Plasmid

    #220751

    Purpose

    Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Insert

    Iron Regulatory Protein (ACO1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV CMV-TERRA

    Plasmid

    #111375

    Purpose

    TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA polyA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NylCA-TS_pET28a

    Plasmid

    #215419

    Purpose

    Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA-TS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S111G/A137L/E263Q

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTol1-UAS:CoChR-tdTomato

    Plasmid

    #124233

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    CoChR-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-H2A-10

    Plasmid

    #55054

    Purpose

    Localization: Nucleus/Histones, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    H2A (H2AC13 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Flag-cIAP1/pRK5

    Plasmid

    #27972

    Depositor

    Xiaolu Yang

    Article

    Hu et al J Biol Chem. 2003 Mar 21;278(12):10055-6

    Insert

    CIAP1 (BIRC2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pH2B-miRFP670nano

    Plasmid

    #127438

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    H2B-miRFP670nano (H2BC11 Synthetic, Human)
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pScalps_Puro

    Plasmid

    #99636

    Purpose

    Empty backbone, 3rd generation lentiviral vector

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSII426

    Plasmid

    #35470

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EF.GFP

    Plasmid

    #17616

    Depositor

    Linzhao Cheng

    Article

    Cui et al Blood. 2002 Jan 15. 99(2):399-408.

    Insert

    EF-1a promoter (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL4.20-HIF1A prom

    Plasmid

    #40173

    Depositor

    Alex Minella

    Article

    Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

    Insert

    HIF1A promoter fragment (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJFRC28-10XUAS-IVS-GFP-p10

    Plasmid

    #36431

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    synthetic U6 promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCherry-H3-23

    Plasmid

    #55058

    Purpose

    Localization: Nucleus/Histones, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    H3 (H3C2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA_DENV2-NS2B3_V5

    Plasmid

    #115906

    Purpose

    Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

    Depositor

    Alan Rothman

    Article

    Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

    Insert

    DENV2 NS2B3 protease
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge