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Showing: 501 - 550 of 8624 results

  5. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12257-plasmid-map-sequence-id-237089

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  6. pCMVR8.74

    Plasmid
    #22036
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Insert
    gag pol tat rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22036-plasmid-map-sequence-id-339901

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  7. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87423-plasmid-map-sequence-id-173982

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  11. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

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  13. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid
    #149276
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Promoter
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    149276-plasmid-map-sequence-id-300510

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  17. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid
    #84561
    Purpose
    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops
    Depositor
    Robert Singer
    Article
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    Use
    Lentiviral
    Tags
    Flag, Suntag, oxBFP, AID (auxin-inducible degron)
    Expression
    Mutation
    Promoter
    UbC
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    84561-plasmid-map-sequence-id-331246

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  20. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  22. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  24. AICSDP-4:TUBA1B-mEGFP

    Plasmid
    #87421
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87421-plasmid-map-sequence-id-441601

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  33. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138150-plasmid-map-sequence-id-350242

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  42. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

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  45. mCherry-CD81-10

    Plasmid
    #55012
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD81 (CD81 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55012-plasmid-map-sequence-id-147992

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  47. tet-pLKO-sgRNA-puro

    Plasmid
    #104321
    Purpose
    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.
    Depositor
    Nathanael Gray
    Article
    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    104321-plasmid-map-sequence-id-323839

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Showing: 501 - 550 of 8624 results