9,060 results
-
-
pSpCTRE-CD4
Plasmid#114010PurposeDox-inducible Cas9 expression with a CD4 selection markerDepositorInsertSpCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-XZ066-EGFRvIII
Plasmid#20737DepositorInsertEGFRvIII (EGFR Human)
UseRetroviralExpressionMammalianMutationEGFR with a 267 amino acid deletion in the extrac…Available SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-N1
Plasmid#53976PurposeLocalization: N1 Cloning Vector, Excitation: 487, Emission: 509DepositorTypeEmpty backboneTagsmEmeraldExpressionMammalianAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1_blasti
Plasmid#194067Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP-T2A-Puro
Plasmid#72263PurposeExpress copGFP and puromycin resistance gene under EF1 promoterDepositorInsertcopGFP-T2A-Puro
UseLentiviralExpressionMammalianPromoterelongation factor-1Available SinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB2
Plasmid#228868PurposePE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequenceDepositorInsertPE7-2A-MLH1-SB-NLS-2A-MLH1-SB
UseCRISPRAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189923PurposeAAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVMutationnSaCas9 KKH, TadA ABE8e V106WPromoterEFSAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9
Plasmid#50661PurposeDoxycycline-inducible lentiviral expression of SpCas9DepositorInserthumanized S. pyogenes Cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianPromoterTet ONAvailable SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dyn2-pmCherryN1
Plasmid#27689DepositorAvailable SinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFP
Plasmid#80491Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expressionDepositorInsertCAG-GFP
UseDonor vector (human)ExpressionMammalianPromoterCAGAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-FLEX-EGFP-WPRE
Plasmid#51502PurposeCan be used to generate AAV virus that will express EGFP in the presence of CreDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pA-His6-Tn5
Plasmid#171871PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase
TagsHis-tagExpressionBacterialMutationWTAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK19-WT-IRES-ZsGreen
Plasmid#68858PurposeExpresses Human CDK19 Wild-TypeDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianPromoterEF1αAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 alpha
Plasmid#20351DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
TagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralExpressionMammalianPromoterEF1 and PGKAvailable SinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 -EGFP
Plasmid#165108Purposemammalian expression and localizationDepositorAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-SPOTlight-U2GCR
Plasmid#164819PurposeReporter to measure ISR state-dependent protein synthesisDepositorInsertSPOTlight-U2GCR
UseAAVExpressionMammalianPromoterCAGAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FlpO
Plasmid#89574PurposeExpresses FlpO under pCAGDepositorInsertFlpO
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloPromoterSFFVAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
gag/pol
Plasmid#14887DepositorInsertgag / pol
UseRetroviralExpressionMammalianAvailable SinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2D1
Plasmid#15782DepositorInsertUbiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
Tags6x His, T7 tag, and Xpress tag (enterokinase clea…ExpressionBacterialAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
ExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1
Plasmid#13798DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
p2attPC
Plasmid#51547PurposeDICE donor plasmid without neural transcription factors.DepositorInsertmCherry
ExpressionMammalianAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRE112
Plasmid#43828DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1ExpressionBacterialAvailable SinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX311 Luciferase
Plasmid#117735PurposeOpen reading frame vector encoding LuciferaseDepositorInsertfirefly Luciferase
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-KRAB-dCas9
Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bx
Plasmid#51552PurposeExpresses Bxb1 integrase in mammalian cells.DepositorInsertBxb1 integrase
ExpressionMammalianPromoterCMVAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP7 (1-220)
Plasmid#42316DepositorAvailable SinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR V7
Plasmid#86856PurposeFluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)DepositorInsertAndrogen receptor (AR) (AR Human)
TagsEGFPExpressionMammalianMutationAlternative splice variant 7 (alteration/deletion…PromoterCMVAvailable SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS-kI
Plasmid#51553PurposeMammalian phiC31 integrase expression vector. For use in applications where pseudosite integration is not desired.DepositorInsertphiC31 integrase
ExpressionMammalianAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.Available SinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsExpressionMammalianPromoterPcmvAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6s
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Alpha-Actinin-19
Plasmid#54975PurposeLocalization: Alpha Actinin, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Raichu-RhoA-CR
Plasmid#40258DepositorInsertRaichu-RhoA-CR (RHOA Human, Synthetic)
ExpressionMammalianAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
LV-Cre pLKO.1
Plasmid#25997PurposeLentiviral expression of nls-Cre from the CMV promoterDepositorInsertCre recombinase
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Klf4
Plasmid#20322DepositorAvailable SinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA
Plasmid#39860DepositorInsertmonomeric streptavidin
Tags6xHis and FLAGExpressionBacterialPromoterT7Available SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-TERT
Plasmid#51631PurposeMammalian expression of 3xFLAG tagged TERT, N-terminal.DepositorAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D1R
Plasmid#89160PurposeExpress vaccinia virus D1R in mammalian cellsDepositorInsertVaccinia virus D1R
ExpressionMammalianPromoterCAG promotorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only
