19,210 results

1. pCDNA3_V5-APEX2-HMOX1

   Plasmid

   #85824

   Purpose

   Expressed N-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   V5-APEX2-HMOX1 (HMOX1 Synthetic, Human)
   

   Use

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Use

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFUSE-rIgG-Fc2-ICAM1

   Plasmid

   #156462

   Purpose

   Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

   Depositor

   Dirk Mielenz

   Article

   Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

   Insert

   Intercellular Adhesion Molecule 1 (Icam1 Mouse)
   

   Use

   Tags

   ICAM1-rFc

   Expression

   Bacterial and Mammalian

   Mutation

   endogenous signal peptide replaced with IL2 signa…

   Promoter

   hEF1-HTLC

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBOB-CAG-SARS-CoV2-Spike D614G-HA

   Plasmid

   #158761

   Purpose

   3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Spike D614G

   Promoter

   CAG

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
   

   Use

   Tags

   NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FoxO1-ADA-GFP

   Plasmid

   #35640

   Depositor

   Domenico Accili

   Article

   Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-4

   Insert

   FoxO1-ADA-GFP (Foxo1 Mouse)
   

   Use

   Tags

   Flag, GFP, and Myc

   Expression

   Mammalian

   Mutation

   T24A, S253D and S316A mutations

   Promoter

   CMV

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PMP34-mCherry-EGFP-Tet-On

   Plasmid

   #109017

   Purpose

   Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCherry-FRB

   Plasmid

   #25920

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

   Insert

   FRB (MTOR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWPT-PDX1

    Plasmid

    #12256

    Depositor

    Didier Trono

    Article

    Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

    Insert

    Pdx1 (PDX1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Str-KDEL_ST-SBP-mCherry

    Plasmid

    #65265

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmCherry-N1-GalT

    Plasmid

    #87327

    Purpose

    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEB1029

    Plasmid

    #22066

    Depositor

    Emmanuelle Bouveret

    Article

    Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

    Type

    Empty backbone

    Use

    Tags

    T25-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid

    #72259

    Purpose

    Express FLPe and tdTomato under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEBTet_NAD-Snifit-cyto

    Plasmid

    #113914

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-HA GST RagC wt

    Plasmid

    #19304

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding C (RRAGC Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGFP-ATIC

    Plasmid

    #99110

    Purpose

    Expresses human GFP-ATIC fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    ATIC (ATIC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S65T mutation in GFP gene

    Promoter

    CMV

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FSW-HRP-TM

    Plasmid

    #82540

    Purpose

    Lentiviral vector for expressing HRP-TM in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    HRP fused to TM domain of PDGFR
    

    Use

    Lentiviral

    Tags

    HRP

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE hBRM

    Plasmid

    #1961

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIRES2-EGFP-deltaNp53:WTp53

    Plasmid

    #49244

    Purpose

    expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN and p53 WT (TP53 Human)
    

    Use

    Tags

    His and IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CFP-CaaX

    Plasmid

    #155232

    Purpose

    membane marker

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWZL Blast DN E-cadherin

    Plasmid

    #18800

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    Dom neg E-cadherin (Cdh1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    truncated form of E-cadherin (dominant negative) …

    Promoter

    Available since

    July 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-BLM

    Plasmid

    #80070

    Purpose

    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

    Depositor

    Nathan Ellis

    Article

    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    If correct ID, the cDNA is from HeLa and has char…

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-HisYFP-Sp100

    Plasmid

    #53141

    Purpose

    E.coli expression of HisYFP tagged human Sp100 fragment

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    Sp100A fragment (SP100 Human)
    

    Use

    Tags

    His-YFP

    Expression

    Bacterial

    Mutation

    fragment (amino acids 71-480); deletion of L75

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLH-sgRNA1-2XMS2

    Plasmid

    #75389

    Purpose

    sgRNA1-2XMS2

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dronpa-Lifeact-7

    Plasmid

    #54740

    Purpose

    Localization: Actin, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

    Type

    Empty backbone

    Use

    Tags

    Dronpa-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pMRXIP-GFP-hATG2A

    Plasmid

    #114462

    Purpose

    Expresses human ATG2A with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tamura et al FEBS Lett. 2017 Dec;591(23):3819-3830. d

    Insert

    human ATG2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P656R (please see depositors comment below)

    Promoter

    CMV

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Synapsin-cyto-iATPSnFR1.0

    Plasmid

    #102556

    Purpose

    expresses cytoplasmic ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hRad51(S208E-A209D)–Cas9(D10A)

    Plasmid

    #125561

    Purpose

    Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(S208E A209D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MSCVhygro-F-G9a

    Plasmid

    #41721

    Purpose

    Retroviral expression of human G9a in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9a (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1(+) Drp1 V2

    Plasmid

    #44599

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    Drp1 variant 2 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBx-Spas-sgRNA-Kan

    Plasmid

    #105233

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. VCP(A232E)-EGFP

    Plasmid

    #23973

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    A232E

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMVMagFRET-1

    Plasmid

    #50742

    Purpose

    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28CLY3

    Plasmid

    #21762

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

39. LentiV_Blast

    Plasmid

    #111887

    Purpose

    Lentiviral expression of cDNA with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA3 Flag p38 beta2 (agf)

    Plasmid

    #20356

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 beta(agf) (MAPK11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 182 and Tyr 184 with Ala and Phe resp…

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

41. pUAS-syb:spGFP1–10

    Plasmid

    #73698

    Purpose

    Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spGFP1–10 chimera
    

    Use

    Drosophila

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-DN-hCUL5-FLAG

    Plasmid

    #15823

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N441

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CMV::SypHy A4

    Plasmid

    #24478

    Depositor

    Leon Lagnado

    Article

    Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

    Insert

    SypHy (Syp Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMIND

    Plasmid

    #24730

    Depositor

    Brian Robertson

    Article

    Blokpoel et al Nucleic Acids Res. 2005 . 33(2):e22.

    Insert

    TetRO promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Promoter

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDSG419

    Plasmid

    #115600

    Purpose

    surface-displayed Ag2; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLEX305_FKBP12F36V-SHOC2

    Plasmid

    #134522

    Purpose

    Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

    Depositor

    Andrew Aguirre

    Article

    Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

    Insert

    SHOC2 (SHOC2 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12F36V-2xHA

    Expression

    Mammalian

    Mutation

    Silent mutations made at wobble base position at …

    Promoter

    hPGK

    Available since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PRS303-240xtetO

    Plasmid

    #44754

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    240x tetO array and CHRI homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRDA_426

    Plasmid

    #179097

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Myo1E-pmAppleC1

    Plasmid

    #27698

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Myo1E (Myo1e Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only