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pLKO.1 neo
Plasmid#13425Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Thioredoxin TEV LIC cloning vector (2T-T)
Plasmid#29712DepositorTypeEmpty backboneUseTagsHis6-Thioredoxin-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND FlpDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 neo
Plasmid#98292PurposeVariant of lentiCRISPRv2 that confers G418 resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLIK DN Rac1
Plasmid#84642PurposeLentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of VenusDepositorInsertRac1 (RAC1 Human)
UseLentiviralTagsExpressionMammalianMutationDominant NegativePromoterAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a
Plasmid#113431PurposeExpresses 10xHis-MBP fused to LbCas12aDepositorHas ServiceCloning Grade DNAInsertLbCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XFLAG-EGFP-OMP25
Plasmid#83354PurposeFor tagging mitochondria with Sigma FLAG epitopesDepositorInsert3XFLAG-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
UseTagstdTomato-v5-GCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (2G-T)
Plasmid#29707DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA
Plasmid#55202PurposePlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (2M-T)
Plasmid#29708DepositorTypeEmpty backboneUseTags6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-PIP-FUCCI
Plasmid#138715PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lifeact-7
Plasmid#54491PurposeLocalization: Action, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsmCherry-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8s
Plasmid#162371PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8s
UseTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/cMG
Plasmid#184539PurposeAAV packaging plasmid, expressing Rep2 and AAV-cMG capsidDepositorInsertAAV-cMG capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R, A589D; insertion o…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_AGIA-AirID
Plasmid#182210PurposeExpress AirID in mammalian cells.DepositorInsertAGIA-AirID
UseTagsAGIA-tagExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-pHluorin_M153R-CD63-mScarlet
Plasmid#172118PurposeDual-color fluorescent reporter for CD63-positive exosome secretion and uptakeDepositorInsertCD63 (CD63 Human)
UseLentiviralTagsmScarlet and pHluorin_M153R in a small extracellu…ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Talin-CS
Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSK664
Plasmid#205130PurposeExpression of GFP-2xPX (from SnxA) in mammalian cellsDepositorInserttandem 2xPX domain of SnxA
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-hLC3B-pcDNA3.1
Plasmid#40827DepositorInsertLC3B (MAP1LC3B Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GFP
Plasmid#86537PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralTagsExpressionMammalianMutationPromoterSynapsin I (human)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1
Plasmid#155346PurposeExpress full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF1 (YTHDF1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABEmax
Plasmid#112095PurposeA:T-to-G:C base editingDepositorInsertABEmax
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-CENP-A-mCherry
Plasmid#89767PurposeLentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-ADepositorInsertCENP-A (CENPA Human)
UseLentiviralTags3xHA and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_H2A_C1
Plasmid#85053PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NbV5-eGFP
Plasmid#201473PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorInsertNbV5-eGFP
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet1
Plasmid#60938PurposeExpresses Tet1 in mammalian cellsDepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Strep-KIFC1*-mCh
Plasmid#120169PurposeExpresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)DepositorInsertkinesin family member C1 (KIFC1 Human)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIFC1: 125-673 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 puro
Plasmid#98290PurposeVariant of lentiCRISPRv2 that confers puromycin resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFR_CrPV_xb
Plasmid#11509DepositorInsertFirefly luciferase, Cricket Paralysis Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagExpressionMutationPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
scAAV-hIBA1a-GFP
Plasmid#214146PurposeSelf-complementary AAV vector packaging GFP under the human Iba1 promoterDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-Bag6
Plasmid#61836Purposeexpress N-terminally FLAG tagged human Bag6DepositorInsertBag6 (BAG6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-Scrambled
Plasmid#47541PurposeTet inducible scrambled shRNA.DepositorInsertScrambled shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-AP-1-mCherry
Plasmid#118095PurposeFluorescent reporter for AP-1 activationDepositorInsertmCherry
UseRetroviralTagsExpressionMutationPromoterminimal promoter with AP-1 responsive elementAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0
Plasmid#153285PurposeExpress the adenosine sensor GRAB_Ado1.0 in neuronsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
UseTags6xHis, pelB leader, and tri-FLAGExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL
Plasmid#66836PurposeExpression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.DepositorInsert5ptaseOCRL (OCRL Human)
UseTagsmCherry-CRY2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G3BP1-WT
Plasmid#135997PurposeWT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
