159,238 results

1. gag-COM-pol

   Plasmid

   #211373

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-COM-pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mTagRFP-T-Lifeact-7

   Plasmid

   #54586

   Purpose

   Localization: Actin, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   LifeAct -mTagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-Frankenbody-FLAG-mRuby2

   Plasmid

   #175294

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mRuby2
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK-ATF4

   Plasmid

   #26114

   Depositor

   Yihong Ye

   Article

   Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti6-H2B-mCherry

   Plasmid

   #89766

   Purpose

   Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   Histone H2B (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CaMKAR

   Plasmid

   #205315

   Purpose

   Fluorescent Reporter for CaMKII Activity

   Depositor

   Mark Anderson, Elizabeth Luczak

   Article

   Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

   Insert

   CaMKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. COM-epegRNA-Dnmt1

    Plasmid

    #211376

    Purpose

    Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    COM-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE8e(TadA-8e V106W)

    Plasmid

    #138495

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFB2B

    Plasmid

    #195284

    Purpose

    Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-TurboID

    Plasmid

    #124646

    Purpose

    Expresses Flag-TurboID fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid

    #149276

    Purpose

    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    T2318C (DNA) in PEBV coat protein sequence

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMIG-FLAG-MLL-AF9

    Plasmid

    #71443

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-Af9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEM-3z/601

    Plasmid

    #26656

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Insert

    
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hsyn-GRAB_ATP1.0

    Plasmid

    #167577

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF3550

    Plasmid

    #145026

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.TBG.PI.eGFP.WPRE.bGH

    Plasmid

    #105535

    Purpose

    AAV expression of EGFP from TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiCMV-Blast

    Plasmid

    #125133

    Purpose

    Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29a-YS14

    Plasmid

    #66890

    Purpose

    Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

    Depositor

    Jung-Hyun Min

    Article

    Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

    Inserts

    His6-Thrombin-Xenopus laevis histones H2A
    Xenopus laevis histones H2B
    Xenopus laevis histones H3
    Xenopus laevis histones H4-Thrombin
    

    Use

    Tags

    H2A, H2B, H3, H4, His6 tag, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge