161,398 results

1. pCI H2B-RFP

   Plasmid

   #92398

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-NCad-GA

   Plasmid

   #191666

   Purpose

   ddGFP-A-inserted mouse N-cadherin

   Depositor

   Takeharu Nagai

   Article

   Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

   Insert

   N-cadherin
   

   Use

   Tags

   HA and ddGFP-A

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken β-actin promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

   Plasmid

   #208401

   Purpose

   AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Lee et al Science. 2023 Oct 13;382(6667):eadf6484.

   Insert

   SYFP2
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   eHGT_354h (enhancer)

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CLYBL-TO-PAX7

   Plasmid

   #204346

   Purpose

   Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   human PAX7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS-NICD

   Plasmid

   #26891

   Depositor

   Nicholas Gaiano

   Article

   Dang et al Dev Neurosci. 2006;28(1-2):58-69.

   Insert

   NICD (Notch1 Mouse)
   

   Use

   Tags

   FLAG and b-globin polyA

   Expression

   Mammalian

   Mutation

   E2299K

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

6. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51087

   Purpose

   Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa 1.3 kb

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFPC2-CB2 SH3-

   Plasmid

   #68822

   Purpose

   EGFP tagged Collybistin expression in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

   Insert

   Collybistin (Arhgef9 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AiP1357 - pAAV-AiE2128m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #220643

   Purpose

   AiE2128m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Bosiljka Tasic, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gRNA Cloning Vector Bbs I ver. 2

   Plasmid

   #85586

   Purpose

   An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-hSyn-Axon-jRex-GECO

    Plasmid

    #172714

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jRex-GECO
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-GFP11-Actin

    Plasmid

    #181966

    Purpose

    Human beta actin tagged with GFP11.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    GFP11-Actin (ACTB Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDF-5xT7-TtCsm

    Plasmid

    #128572

    Purpose

    For expression of T. thermophilus Csm in E. coli (Csm1-5)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Csm1
    Csm2, Csm3, Csm4, Csm5
    

    Use

    Tags

    HRV 3C protease cleavage site and 10xHis tag on C…

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli and Cod…

    Promoter

    T7 promoter, lac operator

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GADD45 WT-luc

    Plasmid

    #8356

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    GADD45 promoter WT (GADD45A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197065

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    I3-01
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pN1-CMV-H-sDarken

    Plasmid

    #184800

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-alpha4 wt

    Plasmid

    #80016

    Purpose

    Mammalian expression of human integrin alpha4

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    integrin alpha4 (ITGA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1_GEM4-Halo-FRB

    Plasmid

    #197057

    Purpose

    Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHes1-DN-GFP (CC#110)

    Plasmid

    #15134

    Depositor

    Connie Cepko

    Article

    Furukawa et al Neuron. 2000 May . 26(2):383-94.

    Insert

    Hes1 (Hes1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E43A, K44A, R47A

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pHes1-DsRed

    Plasmid

    #13767

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Hes1 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDSP33

    Plasmid

    #199375

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. EGFPC2-CB2 SH3+

    Plasmid

    #68821

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-Rab5C-S35N

    Plasmid

    #110503

    Purpose

    Dominant Negative

    Depositor

    Bernardo Mainou

    Insert

    Rab5C-S35N (RAB5C Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S35N

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pHS15 (hANGPTL3-Strep)

    Plasmid

    #109112

    Purpose

    Expresses StrepII-tagged human ANGPTL3 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL3 (ANGPTL3 Human)
    

    Use

    Tags

    StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid

    #197064

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Inserts

    Encapsulin
    Adaptor Protein
    

    Use

    CRISPR

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PA-mCherry-Alpha-5-Integrin-12

    Plasmid

    #62790

    Purpose

    Localization: Focal Adhesions, Excitation:400 / 504, Emission:515 / 517

    Depositor

    Michael Davidson

    Insert

    Alpha5Integrin (ITGAV Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

31. FUGAW

    Plasmid

    #129473

    Purpose

    3rd-generation lentiviral vector for hUbC-driven expression of GFP-aequorin

    Depositor

    Robert Gross

    Article

    Tung et al Neurophotonics. 2016 Apr;3(2):025001. do

    Insert

    GFP-aequorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pN_35S/mEGFP

    Plasmid

    #132565

    Purpose

    mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHS1993

    Plasmid

    #239836

    Purpose

    OMEGAOff-KRK mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    CMV

    Available since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHS2056

    Plasmid

    #239837

    Purpose

    OMEGAOff + omegaRNA scaffold AAV expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    

    Use

    AAV

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    EFS; human U6

    Available since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Tad-CBE(ACA1)

    Plasmid

    #235059

    Purpose

    Expresses Tad-CBE(ACA1) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACA1)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Tad-CBE(ACT4)

    Plasmid

    #235060

    Purpose

    Expresses Tad-CBE(ACT4) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(ACT4)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, SV40NLS and bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1(+)-FLAG-RGS14

    Plasmid

    #236567

    Purpose

    Expresses Flag-RGS14 in mammalian cells

    Depositor

    John Hepler

    Article

    Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

    Insert

    Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJR031

    Plasmid

    #198825

    Purpose

    Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pClodANL-FGF4

    Plasmid

    #107874

    Purpose

    Bacterial expression of FGF4-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    FGF4 (FGF4 Synthetic)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    Promoter

    pro D

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLX304 PCSK5-V5 blast

    Plasmid

    #83100

    Purpose

    Lentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only