165,688 results

1. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-cofilin WT

   Plasmid

   #78295

   Purpose

   Mammalian expression of mouse cofilin fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PSMA4_GFP_Cterm

   Plasmid

   #221514

   Purpose

   Fluorescently tagged Psma4-GFP

   Depositor

   Alessandro Ori

   Insert

   PSMA4 (PSMA4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2RZ

   Plasmid

   #27664

   Depositor

   Graeme Conn

   Article

   Walker et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Type

   Empty backbone

   Use

   Rna transcription

   Tags

   HDV Ribozyme

   Available Since

   April 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hEF1a, U6

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Limk1

   Plasmid

   #163414

   Purpose

   Expresses LIMK1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Limk1 (Limk1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-cofilin-S3A

   Plasmid

   #78296

   Purpose

   Mammalian expression of mouse cofilin S3A (nonphosphorylatable form) fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3A

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. sABE v3.22_N

   Plasmid

   #209553

   Purpose

   pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   TadA-8e(2-76)-2xFRB
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. sABE v3.22_C

   Plasmid

   #209554

   Purpose

   pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   2xFKBP-TadA-8e(77-167)-SpCas9(D10A)
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSV Sport PPAR gamma 2

    Plasmid

    #8862

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Tags

    nls

    Expression

    Mammalian

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBR2k-GFPuv

    Plasmid

    #106383

    Purpose

    Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-Vinexin-C-14

    Plasmid

    #54305

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Vinexin (SORBS3 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

    Plasmid

    #222991

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid

    #196416

    Purpose

    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRF1R-GFP

    Plasmid

    #208912

    Purpose

    encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

    Depositor

    Christian Gruber

    Article

    Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

    Insert

    CRHR1 (CRHR1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. proinsulin-EYFP

    Plasmid

    #218952

    Purpose

    Expresses proinsulin fused to EYFP in bacteria

    Depositor

    Benjamin Glick

    Insert

    proinsulin-EYFP
    

    Tags

    proinsulin

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHYRSF53

    Plasmid

    #64696

    Purpose

    His-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, and NpuDnaE-N102

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-5' MS2-CircRNA

    Plasmid

    #213750

    Purpose

    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-LCK

    Plasmid

    #23890

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    LCK (LCK Human)
    

    Use

    Gateway donor vector

    Mutation

    V93I

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-cofilin-S3D

    Plasmid

    #78297

    Purpose

    Mammalian expression of mouse cofilin S3D fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3D

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pKA-142

    Plasmid

    #79835

    Purpose

    Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    PpsR2-mVenus-CAAX
    

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMVd1

    Available Since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAcBac1-BacMam-4x-PyOtR

    Plasmid

    #217364

    Purpose

    Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    4 copies of pyrrolysyl-tRNA mutant PyOtR
    

    Use

    Baculoviral

    Expression

    Insect and Mammalian

    Promoter

    U6

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR221-SLC7A9_STOP

    Plasmid

    #161411

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pDONR221-SLC7A6_STOP

    Plasmid

    #161375

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A6 (SLC7A6 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pL7Ae-myc-T2A-tagRFP

    Plasmid

    #140263

    Purpose

    Expresses L7Ae in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    L7Ae
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcS-mCherry-C1C2

    Plasmid

    #178425

    Purpose

    Fluorescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    mCherry
    

    Expression

    Mammalian

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MG-155

    Plasmid

    #26529

    Depositor

    David Baltimore

    Article

    O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

    Insert

    miR-155 (MIR155 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

29. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NMBR-Tango

    Plasmid

    #66445

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NMBR (NMBR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

31. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR221-SLC35G6_STOP

    Plasmid

    #161262

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC35G6 (SLC35G6 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pLX307/PTPN11

    Plasmid

    #140941

    Purpose

    expresses PTPN11

    Depositor

    Kimberly Stegmaier

    Article

    Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

    Insert

    PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p18S.2 tag

    Plasmid

    #51731

    Purpose

    transcribes truncated transcript containing 18S and hybridization tag

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    18S
    

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PB-CT3G-ERP2

    Plasmid

    #175497

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pICH47791

    Plasmid

    #48006

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pWZL Neo Myr Flag PAK4

    Plasmid

    #20460

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PAK4 (PAK4 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

39. MG

    Plasmid

    #26528

    Depositor

    David Baltimore

    Article

    O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA-gLuc-C1C2

    Plasmid

    #178426

    Purpose

    Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    gLuc
    

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge