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171,035 results

Showing: 2551 - 2600 of 171035 results

  7. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Expression
    Mammalian
    Promoter
    Tet-On 3G Bidirectional
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  11. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186791-plasmid-map-sequence-id-368060

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  12. pAAV-CaMKIIa-hChR2(H134R)-mCherry

    Plasmid
    #26975
    Purpose
    AAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CaMKIIa
    Available Since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26975-plasmid-map-sequence-id-199793

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  13. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  14. eiABE

    Plasmid
    #227144
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227144-plasmid-map-sequence-id-453987

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  16. pTipi2.1-AZU_SP

    Plasmid
    #229144
    Purpose
    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.
    Depositor
    Institute for Protein Innovation (IPI)
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Jan. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    229144-plasmid-map-sequence-id-457154

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  18. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  20. FUW-C-prM-E-NS1

    Plasmid
    #175281
    Purpose
    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    Use
    Lentiviral
    Tags
    IRES EGFP
    Promoter
    human ubiquitin C
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175281-plasmid-map-sequence-id-349091

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  21. eiCBE

    Plasmid
    #227148
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227148-plasmid-map-sequence-id-453981

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  25. pOpen-MMLV_RT (lack H)

    Plasmid
    #165556
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165556-plasmid-map-sequence-id-339910

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  35. PIP2-CFP(UST) sensor

    Plasmid
    #170357
    Purpose
    PH-CFP(UST) fusion used to measure realtime changes in PIP2 levels.
    Depositor
    Kees Jalink
    Insert
    PH-CFP(UST) (PLCD1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170357-plasmid-map-sequence-id-336129

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  39. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

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  40. pAM007

    Plasmid
    #170011
    Purpose
    Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    Superfolder GFP
    PhoP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTetO-1 and PslyB
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    170011-plasmid-map-sequence-id-335739

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  42. pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP

    Plasmid
    #220828
    Purpose
    Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 35aaNSP1-P2A-NSP5(io)-GS-smURFP
    Use
    Other
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220828-plasmid-map-sequence-id-444017

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  43. pT7-NSP4SA11-hPEST

    Plasmid
    #220820
    Purpose
    Express the Rotavirus SA11 NSP4 fused with the PEST degradation sequence from mouse ornithine decarboxylase
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP4-hPEST
    Use
    Other
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220820-plasmid-map-sequence-id-443936

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  44. pT7-NSP3SA11-F2A-EGFP

    Plasmid
    #220808
    Purpose
    Express separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)
    Depositor
    Alex Borodavka
    Insert
    Rotavirus SA11 NSP3-F2A-EGFP
    Use
    Other
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    220808-plasmid-map-sequence-id-443927

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  49. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  50. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid
    #196227
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn iGluSnFR3 v857.NGR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and NGR TM Domain
    Expression
    Mammalian
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196227-plasmid-map-sequence-id-409161

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Showing: 2551 - 2600 of 171035 results