4,202 results
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pRK7-HA-S6K1-WT
Plasmid#8984DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pX330S-3
Plasmid#58779PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-i_C1
Plasmid#85044PurposeMammalian expression of mScarlet-iDepositorHas ServiceCloning Grade DNAInsertmScarlet-i
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVPromoterhSynapsinAvailable SinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
TagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_sfCherry2(1-10)
Plasmid#82602PurposeExpresses sfCherry2(1-10) fusion in mammalian cellsDepositorInsertsfCherry2(1-10)
ExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
xCas9 3.7
Plasmid#108379PurposeMammalian expression vector for xCas9 3.7DepositorInsertxCas9 3.7
TagsNLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Myc-YAP-5SA
Plasmid#33093DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsMycExpressionMammalianMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…PromoterCMV/MSVAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP hsp70
Plasmid#15215DepositorAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR7-YFP
Plasmid#13022DepositorAvailable SinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRX1 flag
Plasmid#21283DepositorAvailable SinceJuly 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X
Plasmid#75286PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable SinceJuly 18, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-CL1
Plasmid#11950DepositorInsertYFP-CL1
ExpressionMammalianAvailable SinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 2
Plasmid#10672DepositorAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-14-3-3ζ
Plasmid#48798Purposeexpresses human myc-14-3-3ζ in mammalian cellsDepositorAvailable SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-TXNIP
Plasmid#18758DepositorAvailable SinceAug. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
TagsmRFP1ExpressionMammalianPromotercmvAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cdk2-HA
Plasmid#1884DepositorAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-EWS-FLI1
Plasmid#102813PurposeOverexpress EWS-FLI1. 3rd generation lentiviral vector.DepositorAvailable SinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 S HexaPro
Plasmid#154754PurposeMammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variantDepositorInsertSpike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
Tags2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …ExpressionMammalianMutationEctodomain only (AAs 1-1208); 682-685 (furin site…Available SinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianPromoterLTR and PGKAvailable SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSIH1-puro-STAT3 shRNA
Plasmid#26596DepositorInsertSTAT3 shRNA (STAT3 Human)
UseLentiviral and RNAiAvailable SinceNov. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianPromoterCBhAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec16A
Plasmid#36155PurposeExpresses Sec16A as a GFP fusionDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
Tags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianPromoterMSSV_LTRAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV/SB10
Plasmid#24551DepositorInsertSleeping Beauty
ExpressionBacterialAvailable SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TCF1
Plasmid#40620DepositorAvailable SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-TA-ERP2
Plasmid#80477PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
a1Ha-pcDNA3
Plasmid#45809DepositorAvailable SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2
Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
TagsHis6 and XpressExpressionMammalianPromoterCMV IEAvailable SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Myt1l
Plasmid#27152DepositorAvailable SinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.1
Plasmid#42631DepositorAvailable SinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits
