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19,770 results

Showing: 3251 - 3300 of 19770 results

  4. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  6. p414-TEF1p-Cas9-CYC1t

    Plasmid
    #43802
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 promoter
    Available Since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43802-plasmid-map-sequence-id-333570

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  7. mTagRFP-T-Lifeact-7

    Plasmid
    #54586
    Purpose
    Localization: Actin, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Lifeact-mTagRFP-T
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54586-plasmid-map-sequence-id-176185

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  17. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  18. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46569-plasmid-map-sequence-id-364492

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  20. pAAV-EF1a-DIO-mCherry

    Plasmid
    #50462
    Purpose
    Double floxed mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Promoter
    EF1a
    Available Since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50462-plasmid-map-sequence-id-182309

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  29. hTLR4

    Plasmid
    #13086
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (TLR4 Human)
    Expression
    Mammalian
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13086-plasmid-map-sequence-id-142442

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  32. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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  37. pCLX-PGK-hTERT

    Plasmid
    #114315
    Purpose
    Constitutive expression of the hTert protein
    Depositor
    Patrick Salmon
    Insert
    hTERT (TERT Human)
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114315-plasmid-map-sequence-id-218852

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  42. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174665-plasmid-map-sequence-id-344886

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  50. mEmerald-CLIP170-C-18

    Plasmid
    #54043
    Purpose
    Localization: Microtubule Binding , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    CLIP170 (CLIP1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3251 - 3300 of 19770 results