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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,167 results

Showing: 3351 - 3400 of 19167 results

  1. pSBtet-Pur

    Plasmid
    #60507
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60507-plasmid-map-sequence-id-322901

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  3. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

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  16. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Use
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  17. mCherry-CD36-C-10

    Plasmid
    #55011
    Purpose
    Localization: Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD36 (CD36 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55011-plasmid-map-sequence-id-313445

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  27. pCB578

    Plasmid
    #141095
    Purpose
    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
    Depositor
    Chase Beisel
    Article
    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d
    Inserts
    Cas9 and tracrRNA
    repeat-spacer-repeat array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    141095-plasmid-map-sequence-id-292312

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  30. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Article
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymease I Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

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  33. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  35. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71684-plasmid-map-sequence-id-129672

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  37. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

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  39. pDONR223 SARS-CoV-2 NSP12

    Plasmid
    #141265
    Purpose
    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141265-plasmid-map-sequence-id-288081

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  43. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1733-plasmid-map-sequence-id-334

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  46. pICE-HA-MRE11-H129N

    Plasmid
    #82034
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    SĂ©bastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Nuclease-dead mutant: H129N MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82034-plasmid-map-sequence-id-155387

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  48. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

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Showing: 3351 - 3400 of 19167 results