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171,009 results

Showing: 3601 - 3650 of 171009 results

  3. pSpCas9(BB)-2A-Blast

    Plasmid
    #118055
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118055-plasmid-map-sequence-id-443898

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  10. pPIDNB2

    Plasmid
    #169902
    Purpose
    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.
    Depositor
    Richard Schneider
    Article
    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05
    Type
    Empty backbone
    Use
    Avian expression
    Expression
    Mammalian
    Promoter
    Dox inducible
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169902-plasmid-map-sequence-id-334686

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  13. cacna2d1 pcDNA3

    Plasmid
    #206103
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206103-plasmid-map-sequence-id-415385

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  14. TFORF0983

    Plasmid
    #143739
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF5 (KLF5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143739-plasmid-map-sequence-id-407888

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  18. pEGFP rbFOX2

    Plasmid
    #63086
    Purpose
    Mammalian expression of EGFP tagged FOX2
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    RBFOX2 (RBFOX2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63086-plasmid-map-sequence-id-104485

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  22. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  28. pCfB2188-PrARG8-GFP::URA3

    Plasmid
    #216512
    Purpose
    Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrARG8-yeGFP
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    ARG8
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216512-plasmid-map-sequence-id-429384

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  29. pCfB2197-PrGNP1-mK2-NatMX

    Plasmid
    #216513
    Purpose
    Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast
    Depositor
    Juha Saarikangas
    Article
    Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.
    Insert
    PrGnp1-mKate2
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Promoter
    GNP1
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    216513-plasmid-map-sequence-id-429383

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  30. pD649-HAsp-IGSF8-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156912
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IGSF8 (IGSF8 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156912-plasmid-map-sequence-id-444413

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  34. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  41. pVITRO1-Trastuzumab-IgM/κ

    Plasmid
    #61881
    Purpose
    Expresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Mu heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61881-plasmid-map-sequence-id-101188

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  43. pLV-EF1A-CD19.28H.28TM.BB.28.3z

    Plasmid
    #200681
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200681-plasmid-map-sequence-id-392612

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  44. LV-AR-C563A-C596A

    Plasmid
    #171220
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C563A-C596A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C563A-C596A
    Promoter
    EF-1a
    Available Since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171220-plasmid-map-sequence-id-342449

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  45. LV-AR-C619Y

    Plasmid
    #171222
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C619Y (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C619Y
    Promoter
    EF-1a
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171222-plasmid-map-sequence-id-336120

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  48. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available Since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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Showing: 3601 - 3650 of 171009 results