15,002 results

1. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ284-RVRR

   Plasmid

   #160327

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Mb2Cas12a RVRR variant
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N563R, K569V, N573R and K625R

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. hfDECOR-F148A

   Plasmid

   #219546

   Purpose

   Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-F148A
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Phenylalanine 148 to Alanine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hfDECOR-V106G

   Plasmid

   #219547

   Purpose

   Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

   Insert

   hfDECOR-V106G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Changed TadA8e Valine 106 to Glycine

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. OA-986F (AAEL003877-dCas9-VPR)

   Plasmid

   #183993

   Purpose

   Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   AAEL003877-dCAS9-VPR
   

   Use

   CRISPR

   Tags

   dCas9-VPR

   Expression

   Insect

   Promoter

   AAEL003887

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

   Plasmid

   #47550

   Purpose

   Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   ttTi5605 sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Promoter

   Chicken ß-actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJW1285

   Plasmid

   #61252

   Purpose

   CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E) targeting pha-1
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. non-targeting control gRNA (BRDN0001145694)

    Plasmid

    #80191

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid

    #170185

    Purpose

    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Inserts

    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CBh and hU6

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p426_Cas9_gRNA-YPRCd15c

    Plasmid

    #87404

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid

    #198551

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor

    Plasmid

    #211888

    Purpose

    CENP-T[1-242] transgene integration at AAVS1 safe harbor locus

    Depositor

    Iain Cheeseman

    Insert

    CENP-T[1-242] (CENPT Human, Synthetic)
    

    Use

    CRISPR

    Mutation

    T11A, T85A

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AsdCas12a VPR

    Plasmid

    #188512

    Purpose

    Expresses FLAG tagged AsdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA-SpCas9-g5

    Plasmid

    #80788

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid

    #71828

    Purpose

    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    U6

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAN-PTet-dCas9

    Plasmid

    #62244

    Purpose

    controls the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    catalytically inactive, D10A, H840A

    Promoter

    Ptet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pS34.EF1α<GFP-2A-Cas9-RC

    Plasmid

    #207404

    Purpose

    Cas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    Cas9-RC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GFP-2A

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-hA3G-BE3

    Plasmid

    #113415

    Purpose

    Expresses hA3G-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3G-BE3 (APOBEC3G S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. MSP2292

    Plasmid

    #70707

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAL-pck_T

    Plasmid

    #74068

    Purpose

    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (T)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pLV-sgCDKN1B#2 BFP

    Plasmid

    #60905

    Purpose

    A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid

    #99694

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. p-dCas9-G9a-Hygro

    Plasmid

    #104408

    Purpose

    transient expression of dCas9-G9a fusion protein

    Depositor

    Stephan Beck

    Insert

    G9a (EHMT2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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32. CYLBL_Neo_CAGdCas9-KRAB

    Plasmid

    #220439

    Purpose

    CAG-dCas9-KRAB knock-in using TALEN at CLYBL locus

    Depositor

    Yin Shen

    Article

    Yang et al Nat Genet. 2023 Oct;55(10):1735-1744. do

    Insert

    dCas9
    

    Use

    CRISPR and TALEN

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. Gephyrin (px330:2-guideCas9)

    Plasmid

    #169432

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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34. p426_Cas9_gRNA-R-RDS1a

    Plasmid

    #87405

    Purpose

    p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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35. pMIA3-Keppel

    Plasmid

    #214446

    Purpose

    Expresses eSpCas9 and gRNA targeting Keppel-19 GSH

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Insert

    eSpCas9-P2A-mRuby2-P2A-tp53dn
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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36. 1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

    Plasmid

    #109316

    Purpose

    Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Insert

    eGFP gRNA
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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37. pY003 (pFnCpf1_delta Cas)

    Plasmid

    #69974

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Cas genes are deleted from locus

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCascade_SPS1(PmcCAST)

    Plasmid

    #224926

    Purpose

    Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

    Depositor

    Leifu Chang

    Article

    Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

    Inserts

    Cas5, Cas6, Cas7, Cas8, Cas11
    CRISPR assay for crRNA expression
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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40. pET_StrepII_TEV_LIC_TniQ(PmcCAST)

    Plasmid

    #224927

    Purpose

    TniQ expression plasmid for the co-purification of TnsC-TniQ complex

    Depositor

    Leifu Chang

    Article

    Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

    Insert

    TniQ
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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41. LCV2_mClover3

    Plasmid

    #155095

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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42. pN-PITCh-3XHA

    Plasmid

    #127881

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-3XHA (PPP2CA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. p207-Switch-ON (FRT)

    Plasmid

    #217886

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pTPH413

    Plasmid

    #185728

    Purpose

    Bacterial PAM profiling of adenine base editor variants

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Inserts

    ecTadA(8e)-neNme2-C
    Nme2Cas9 sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCOM-CD63-COM

    Plasmid

    #138350

    Purpose

    Expressing COM-CD63-COM fusion protein in mammalian cells

    Depositor

    Baisong Lu

    Article

    Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

    Insert

    COM-CD63-COM fusion protein
    

    Use

    CRISPR and Lentiviral

    Tags

    COM is fused to the N and C termini of human CD63

    Expression

    Mammalian

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSA_0_mKate2_synCoTC

    Plasmid

    #199469

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    mKate2
    

    Use

    CRISPR

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. U6-5' MS2-CircRNA

    Plasmid

    #213750

    Purpose

    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-SMVP-Cas9N-U6-gRNA M3

    Plasmid

    #80936

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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