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173,143 results

Showing: 4751 - 4800 of 173143 results

  7. pMSP3535

    Plasmid
    #46886
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Expression
    Bacterial
    Promoter
    PnisA
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46886-plasmid-map-sequence-id-457466

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  8. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50580-plasmid-map-sequence-id-75637

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  12. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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  16. Lamin A (R435C)-mRFP

    Plasmid
    #124274
    Purpose
    Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R435C) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R435C)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124274-plasmid-map-sequence-id-241955

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  17. ADRA2C-Tango

    Plasmid
    #66218
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2C (ADRA2C Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pFsynW SYT1 reporter

    Plasmid
    #197275
    Purpose
    Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    N/A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197275-plasmid-map-sequence-id-389085

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  23. TFORF2358

    Plasmid
    #142046
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF644 (ZNF644 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    142046-plasmid-map-sequence-id-326359

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  24. pGEX4T1 SHP1 WT

    Plasmid
    #8578
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Expression
    Bacterial
    Available Since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  37. Tagln2-GFP

    Plasmid
    #221530
    Purpose
    Fluorescently tagged Tagln2-GFP
    Depositor
    Alessandro Ori
    Insert
    Tagln2 (TAGLN2 Human)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221530-plasmid-map-sequence-id-439384

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  40. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid
    #246436
    Purpose
    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    Tags
    GFP N-terminal sequence (M1 to Q95), Hemi Intron …
    Expression
    Mammalian
    Mutation
    GFP N-terminal sequence M1 to Glutamine 95 append…
    Promoter
    CMV and U1a
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246436-plasmid-map-sequence-id-490914

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  41. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid
    #246435
    Purpose
    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    246435-plasmid-map-sequence-id-488935

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  44. pQCXIG (w497-1)

    Plasmid
    #26286
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    26286-plasmid-map-sequence-id-12372

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  50. TFORF1115

    Plasmid
    #143092
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RCOR2 (RCOR2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143092-plasmid-map-sequence-id-399033

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Showing: 4751 - 4800 of 173143 results