9,128 results
-
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only
-
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-pgk-puro
Plasmid#85747Purposetetracyline-inducible lentiviral vector with no insert for use in mammalian cellsDepositorTypeEmpty backboneUseLentiviralTagsnonePromoterCMVAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rictor_1 shRNA
Plasmid#1853DepositorAvailable SinceJuly 10, 2005AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
ExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MS2coatProtein
Plasmid#103832PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmCherry-MS2 coat protein (cp )
ExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET mCitrine LIC cloning vector (u-mCitrine)
Plasmid#29771DepositorTypeEmpty backboneTagsmCitrineExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PfV-Sapphire-IRES-DSRed
Plasmid#116934PurposeMammalian lenti vector for expression of the 40nm GEM and DSRedDepositorInsertPfv
UseLentiviralTagsSapphirePromoterCMVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP5G
Plasmid#31788Purposea single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.DepositorInsertGCaMP5G
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.eGFP.2A.wtTau
Plasmid#140424PurposeAAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTauDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-zif268
Plasmid#18045DepositorInsertzif268
ExpressionBacterialMutationZif268 is fused to the omega subunit of RNA polym…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
ExpressionMammalianAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)
Plasmid#85583PurposecGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)DepositorInsertPrab-3-GAL4-SK(DBD)-VP64
ExpressionWormPromoterPrab-3-GAL4-SK(DBD)-VP64Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580AAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ChR2-Venus
Plasmid#15753PurposeExpresses ChR2-Venus for neuronal excitation.DepositorInsertChannelrhodopsin2-Venus
TagsVenusExpressionMammalianPromoterCAGAvailable SinceOct. 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS-Hsp70-Cas9
Plasmid#46294PurposeA codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.DepositorInsertcodon optimized Cas9
UseCRISPRTags3x FlagExpressionInsectMutationSilent change from C to A at bp 834 of insertPromoterHsp70Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMGS7 (AAVS1sgRNA)
Plasmid#126582PurposeA CRISPR/Cas9 construct to target the AAVS1 locus in humansDepositorInsertAAVS1 sgRNA
ExpressionMammalianAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRPromoterEFSAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302 Luciferase-V5 puro
Plasmid#47553PurposeLentiviral expression of V5-tagged Luciferase in mammalian cellsDepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianMutationNonePromoterCMVAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 Wt (1-674aa)
Plasmid#75478Purposemammalian expression of GADD34DepositorAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorInsertRNF2_5GtoT pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
Tags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXs-hKLF4
Plasmid#17219PurposeRetroviral expression of human KLF4DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV
Plasmid#140135PurposeCan be used to generate AAV virus that will express light-inducible site-specific iCreV recombinaseDepositorHas ServiceAAV PHP.eBInsertiCreV
UseAAV and Cre/LoxExpressionMammalianPromoterEF1alphaAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
TagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianPromoterEF-1aAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-c-Met
Plasmid#31784DepositorAvailable SinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom
Plasmid#135630PurposeAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PJ-INPP5E
Plasmid#38001DepositorTagsmRFPExpressionMammalianMutationCys 779 mutated to SerAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-TagRFP-T-IFT88
Plasmid#61684PurposeMammalian expression of IFT88 fused with N terminal TagRFP.T tagDepositorAvailable SinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
pGP-AAV-syn-FLEX-jGCaMP8m-WPRE
Plasmid#162378PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3M14nes
Plasmid#155367PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertUseCRISPRExpressionMammalianMutationPspCas13b Δ984-1090 H133AAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo largeTcDNA
Plasmid#1779DepositorAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-dCas9-KRAB
Plasmid#115545PurposeDoxycycline inducible dCas9-KRAB targeting construct for AAVS1 locusDepositorTypeEmpty backboneTagsHAExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXSN18E6E7
Plasmid#53459PurposeThe plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.DepositorInsertHPV18E6E7
UseRetroviralAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 (pCMV-IRES-GFP version 3)
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianPromoterCMV, lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/BFP Dest
Plasmid#74282PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloningDepositorInsertRosa26 5-homology region
Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
tTA IRES GFP
Plasmid#18783DepositorInserttTA
UseRetroviralTagsIRES GFPExpressionMammalianAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorAvailable SinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-BAIAP2 wt
Plasmid#31656DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA
Plasmid#99698PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAVDepositorTypeEmpty backboneUseAAVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-N1
Plasmid#54568PurposeLocalization: N1 Cloning Vector, Excitation: 549, Emission: 565DepositorTypeEmpty backboneTagsmOrange2ExpressionMammalianAvailable SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
Plasmid#51084PurposeNeuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV Retrograde and AAV1InsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhuman Synapsin1Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only