165,637 results
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Plasmid#26430DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only
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lentiCas9-EGFP
Plasmid#63592PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.DepositorInsertsCas9
EGFP
UseCRISPR and LentiviralTagsFLAG and P2AExpressionMammalianMutationPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo
Plasmid#221899PurposeExpresses alpha tubulin fused to mStayGoldDepositorInsertmStayGold-aTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6d
Plasmid#207854PurposeMammalian expression of PE6d prime editorDepositorInsertPE6d
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrt(T128N, V223Y, D200C)PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
UseTagsExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2BD
Plasmid#234428PurposePlasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning templateDepositorInsertModified Cas9 with polyaspartate linking
UseTagsExpressionBacterialMutationLinked polyaspartate using glycine-serine linkerPromoterAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICH41331::pU6cm-pegR::EURb7
Plasmid#227695PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).DepositorInsertAltered epegRNA backbone with cloning sites for gRNA and RTT.
UseCRISPRTagsExpressionPlantMutationPromoterU6 compositeAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-28z
Plasmid#135991PurposeModular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8001 - pCMV-R8.91 (D64V)
Plasmid#202687PurposeR8.91 plasmid with mutated integrase (D64V)DepositorInsertgag pol
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9-X1.1
Plasmid#196836Purposenon-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV9-X1.1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-CAAX
Plasmid#116856Purposeplasma membrane markerDepositorInsertHRAS
UseTagsTagBFP2ExpressionMammalianMutationAmino acids 172-189PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP (AAV1)
Viral Prep#50457-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-lck-GCaMP6f (AAV5)
Viral Prep#52924-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1 gfaABC1D-lck-GCaMP6f (#52924). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-lck-GCaMP6f plasmid DNA. gfaABC1D-driven (similar to GFAP promoter) lck-GCaMP6f calcium sensor expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIV-D3psi/delta env/delta Vif/delta Vpr
Plasmid#132928PurposeProduction of SIV-VLPs containing Vpx proteinDepositorInsertGag, pol, and vpx
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry (AAV1)
Viral Prep#114472-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac PlayBack
Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)
Viral Prep#73179-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.DepositorPromoterTagsNoneAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE (AAV1)
Viral Prep#176752-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Renilla luciferase
Plasmid#118016PurposeConstitutive expression of Renilla luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
35S:RUBY
Plasmid#160908PurposeRUBY under the control of CaMV 35S promoter/marker for transformationDepositorInsertRUBY and CaMV 35S promoter
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dPspCas13b-3xmRuby3-NLS
Plasmid#132405Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsExpressionMammalianMutationH133A; H1059APromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)
Plasmid#103866PurposedPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP (AAV5)
Viral Prep#50457-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified) (AAV Retrograde)
Viral Prep#59462-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. tdTomato expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (codon diversified)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-gRNA-Actb1216
Plasmid#155368PurposePspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylationDepositorInsertPspCas13b gRNA targeting Actb 1216
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
KW54_HART_ODC1
Plasmid#207398PurposeLentiviral plasmid containing DDX3X reporter HART for ODC1 5UTRDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2
Plasmid#113634PurposeAn expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepaciaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP
Plasmid#230075PurposePartial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterfabp10aAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-FLuc
Plasmid#188311PurposeExpression of Firefly luciferase via a T7 promoter in TXTLDepositorInsertFirefly luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHM066
Plasmid#225524PurposeEncodes Wnt-responsive destablized iRFPDepositorInsertiRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.3b (AAV5)
Viral Prep#125560-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
GCH1_pLX307
Plasmid#98336PurposeLentiviral expression of GCH1DepositorInsertGCH1 (GCH1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)
Viral Prep#83897-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor
Plasmid#202689PurposeCLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.DepositorInsertsCopGFP
mCherry
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8079 - IL2RG GFP-P2A CLIP Donor
Plasmid#202690PurposeCLIP donor template for integrating GFP into IL2RG locus.DepositorInsertCopGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
KW57_HART_RAC1
Plasmid#207399PurposeLentiviral plasmid containing DDX3X reporter HART for RAC1 5UTRDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-PE2max-NC::tEURb7
Plasmid#227693PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette.DepositorInsertPE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f
Plasmid#182548PurposeExpresses GCaMP6f on the cytosolic side of the ER (P-450site)DepositorInsertER localized P450
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-Benzonase
Plasmid#218192PurposeBacterial expression of a periplasmically expressed MBP-tagged Serratia Marcescens NucA (Benzonase); also known as c001DepositorInsertnucA
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceApril 24, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
paavCAG-iCre
Plasmid#51904PurposeFor AAV expression of iCre recombinaseDepositorInsertimproved Cre recombinase
UseAAV and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
MoClo Yeast Toolkit
Plasmid Kit#1000000061Purpose96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettesDepositorAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bxb1
Plasmid#182142PurposeExpression of human codon-optimized Bxb1DepositorInserthuman codon optimized Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2
Plasmid#132775PurposePrime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPE2
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC control
Plasmid#10879PurposeNegative control shRNA vector containing non-hairpin insert.DepositorInsertnon-hairpin 18bp
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only