293,398 results

1. pCDNA3.1-Nanog

   Plasmid

   #28221

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Jan 18. ():.

   Insert

   Nanog (NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFPC1-FLAG-Synaptojanin 1-145

   Plasmid

   #22293

   Depositor

   Pietro De Camilli

   Article

   Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

   Insert

   Synaptojanin 1_145 (SYNJ1 Human)
   

   Use

   Tags

   Flag and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDR195

   Plasmid

   #36028

   Depositor

   Wolf Frommer, Dominique Loque

   Article

   Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC1.509

   Plasmid

   #203955

   Purpose

   Level 1 self-replicating vector containing a DAPG inducible FnCas12a

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   PphlF-Cpf1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDH-EFIa-Id1-PGK-puro

   Plasmid

   #107735

   Purpose

   Expresses Id1 in mammalian cells

   Depositor

   Alexandre Colas

   Article

   Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

   Insert

   Id1 (T Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFP.E222

   Plasmid

   #138248

   Purpose

   Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   ECF20.N.(VDA).M86.N2
   Terminator
   SspGyrB.C2.(SAK).ECF16.C
   Terminator
   rhaS
   ECF17.N.(NPC).NrdJ-1.N2
   Terminator
   M86.C2.(SDL).ECF20.C
   Terminator
   luxR
   Terminator
   ECF16.N.(AGG).SspGyrB.N2
   Terminator
   NrdJ-1.C2.(SEI).ECF17.C
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NW50

   Plasmid

   #133445

   Purpose

   Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

   Depositor

   Gerhard Wagner

   Article

   Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

   Insert

   NW50
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BL21ΔCF

   Bacterial Strain

   #102265

   Purpose

   ompC, ompF double knockout strain for overproduction of outer membrane proteins

   Depositor

   Jack Leo

   Article

   Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

   Bacterial Resistance

   None

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pJW2098

    Plasmid

    #163094

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+ GCamp6-3NLS

    Plasmid

    #165401

    Purpose

    Expresses a nuclear localized genetically encoded calcium reporter.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    GCamp6

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEB-Tg

    Plasmid

    #28220

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    SV40 large T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p-dCas9-LSD1-Hygro

    Plasmid

    #104406

    Purpose

    transient expression of dCas9-LSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    LSD1 (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLXRN-EGFP

    Plasmid

    #99207

    Purpose

    EGFP expressing bicistronic retroviral vector

    Depositor

    Peter Pedersen

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A Kozak sequence was incorporated to enhance tran…

    Promoter

    LTR

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIDK-HDAC1fl

    Plasmid

    #109158

    Purpose

    Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

    Pooled Library

    #159393

    Purpose

    Mouse lentiviral genome-wide CRISPR library

    Depositor

    Jason Moffat

    Article

    Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pEP4 E02S EN2L

    Plasmid

    #20922

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Nanog and Lin28 (POU5F1, SOX2, NANOG, LIN28A Human)
    

    Use

    Episomal/ebna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti6.3-hEPO

    Plasmid

    #50436

    Purpose

    express human erythropoietin using lentiviral delivery system

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

    Insert

    EPO (EPO Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA-R-iLACCO1.1

    Plasmid

    #208026

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NanoLuc-PM-Venus

    Plasmid

    #164786

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    

    Use

    Tags

    NanoLuc and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2

    Plasmid

    #64512

    Purpose

    Expression of St1 dCas9-3XTagBFP2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pZa pBAD csgA CBDcex pLac Ara csgGEF

    Plasmid

    #226378

    Purpose

    Plasmid carries csgA and csgG,E,F genes of curli operon

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    csgA and csgG,E,F genes of curli operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-ICUE3

    Plasmid

    #61622

    Purpose

    FRET-based biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    ICUE3 (RAPGEF3 Human)
    

    Use

    Tags

    ECFP and cpVenus[L194]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

    Plasmid

    #187576

    Purpose

    Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    LMNA (LMNA Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. iBE4max YE1 NG CLYBL

    Plasmid

    #174570

    Purpose

    doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet ON

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FLAG-MED17

    Plasmid

    #15410

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

    Insert

    MED17 (MED17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Mouse Validation (mVAL) CRISPR Library

    Pooled Library

    #159391

    Purpose

    Pooled CRISPR library targets core intrinsic immune evasion genes

    Depositor

    Jason Moffat

    Article

    Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDuet TDP-43 Q331K

    Plasmid

    #27465

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMU-GAr

    Plasmid

    #61172

    Purpose

    Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    GmMAN49-mCherry
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFB1.HMBP.PrS.EZH2

    Plasmid

    #125161

    Purpose

    Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGW_GPHN.FingR-YFP

    Plasmid

    #46298

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR (GPHN Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

    Plasmid

    #167901

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28-hTDG

    Plasmid

    #70758

    Purpose

    bacterial expression of human TDG

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    TDG (TDG Human)
    

    Use

    Tags

    6-HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lac operon

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmr101A-hVPS26

    Plasmid

    #17636

    Depositor

    James Hurley

    Article

    Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

    Insert

    VPS26 (VPS26A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMaster3

    Plasmid

    #58526

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor

    Plasmid

    #229788

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF10 and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. EF.hICN1.Ubc.GFP

    Plasmid

    #17626

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCruzHA SIRT1

    Plasmid

    #10962

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-SUN1_916

    Plasmid

    #125850

    Purpose

    Expresses SUN1_916 tagged with GFP in mammalian cells

    Depositor

    Miki Hieda

    Article

    Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

    Insert

    SUN1_916 (SUN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    stop codon

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCDHblast MCSNard OST-LMNAd50

    Plasmid

    #22662

    Depositor

    Tom Misteli

    Article

    Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

    Insert

    LaminAd50 (=Progerin)
    

    Use

    Lentiviral

    Tags

    Onestrep Tag

    Expression

    Mammalian

    Mutation

    50AA deletion creating Progerin instead of mature…

    Promoter

    Available since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRSET FLIPE-600n

    Plasmid

    #13537

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E WT
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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46. pHCMV_SARS-CoV-2_Sdel18

    Plasmid

    #207307

    Purpose

    Mammalian expression of SARS2 coronavirus S protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SARS-CoV2_S (S SARS2 coronavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

    Plasmid

    #187575

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCXLE-hGLIS1

    Plasmid

    #37625

    Purpose

    Non-integrating (episomal) expression of human GLIS1

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    human GLIS1 (GLIS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T110A, A187G, I221T

    Promoter

    CAG

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCDH_blast_MCS_Nard_GFP_D50

    Plasmid

    #167339

    Purpose

    Expresses GFP fused to progerin (d50) in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    Progerin
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    50AA deletion creating Progerin instead of mature…

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pflareG-psd95-exon18

    Plasmid

    #90266

    Purpose

    Minigene reporter for alternative splicing analysis

    Depositor

    Douglas Black

    Article

    Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

    Insert

    Psd95-exon18 (Dlg4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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