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179,463 results

Showing: 601 - 650 of 179463 results

  4. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    41732-plasmid-map-sequence-id-210671

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  8. mEGFP-N1

    Plasmid
    #54767
    Purpose
    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    43 Articles
    54767-plasmid-map-sequence-id-204493

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  19. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Actin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    mCherry-Lifeact
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    56 Articles
    54491-plasmid-map-sequence-id-227430

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  24. pAAV-hSyn-EGFP

    Plasmid
    #50465
    Purpose
    EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222 Articles
    50465-plasmid-map-sequence-id-240907

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  34. pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)

    Viral prep
    #107738-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    June 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  38. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    61408-plasmid-map-sequence-id-333613

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  47. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Expression
    Mammalian
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    10676-plasmid-map-sequence-id-139182

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  48. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV Retrograde)

    Viral prep
    #20298-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Oct. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  50. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Publication
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    61737-plasmid-map-sequence-id-190162

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Showing: 601 - 650 of 179463 results