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20,807 results

Showing: 10851 - 10900 of 20807 results

  1. pSH62-EBD

    Plasmid
    #49455
    Purpose
    yeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinase
    Depositor
    Marc Gartenberg
    Article
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    Cre-EBD fusion
    Use
    Cre/Lox
    Expression
    Yeast
    Promoter
    Gal1 promoter
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49455-plasmid-map-sequence-id-73668

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  6. pT2/BH

    Plasmid
    #26556
    Depositor
    Perry Hackett
    Insert
    pT2/BH
    Expression
    Bacterial
    Available Since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26556-plasmid-map-sequence-id-203999

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  8. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR A757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140829-plasmid-map-sequence-id-305143

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  10. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

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  11. TNFR1-YFP

    Plasmid
    #111209
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1A (TNFRSF1A Human)
    Tags
    EYFP
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111209-plasmid-map-sequence-id-212987

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  14. pT2/SV-Venus-SIINFEKL

    Plasmid
    #111624
    Purpose
    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies
    Depositor
    Jon Yewdell
    Insert
    Venus-LEQLE-SIINFEKL-TEW
    Tags
    LEQLE-SIINFEKL-TEW
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111624-plasmid-map-sequence-id-213225

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  23. pSicoR

    Plasmid
    #11579
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    11579-plasmid-map-sequence-id-251845

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  25. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  33. ins:CFP-NTR

    Plasmid
    #66593
    Purpose
    Specific and conditional cell ablation of beta cells in the zebrafish pancreas
    Depositor
    Didier Stainier
    Article
    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.
    Insert
    Insulin promotor driving CFP-nitroreductase
    Use
    Zebrafish expression
    Tags
    CFP fused with N-terminus of nitroreductase
    Promoter
    Zebrafish Insulin promoter (approximately 1.2kb)
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  47. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

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Showing: 10851 - 10900 of 20807 results