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Mammalian Expression

76,289 results

Showing: 5751 - 5800 of 76289 results

  1. pCAG-VSVP

    Plasmid
    #64088
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV phosphoprotein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64088-plasmid-map-sequence-id-438903

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  4. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51087
    Purpose
    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Available since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    51087-plasmid-map-sequence-id-77369

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  5. GPR87-Tango

    Plasmid
    #66379
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR87 (GPR87 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66379-plasmid-map-sequence-id-419752

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  6. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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  7. pCLIP-tag (m) Vector

    Plasmid
    #101136
    Purpose
    Expression of protein of interest with CLIP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    CLIP-tag (CLIP10)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101136-plasmid-map-sequence-id-194177

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  10. pHRIG-Akt3

    Plasmid
    #53596
    Purpose
    lentiviral expression of constitutively active human Akt3 and EGFP
    Depositor
    Heng Zhao
    Article
    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12
    Insert
    cAkt3 (AKT3 Human)
    Use
    Lentiviral
    Tags
    HA and IRES-EGFP
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pStr-KDEL_TPV-CXCL9-mCherry-SBP

    Plasmid
    #170677
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    CXCL9 (CXCL9 Human, Synthetic)
    streptavidin-KDEL
    Use
    Tags
    SBP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170677-plasmid-map-sequence-id-335797

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  18. TFORF0214

    Plasmid
    #142486
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP1 (FOXP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142486-plasmid-map-sequence-id-397235

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  24. TFORF2242

    Plasmid
    #141986
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA1 (GATA1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    141986-plasmid-map-sequence-id-395891

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  26. CLYBL-TO-PAX7

    Plasmid
    #204346
    Purpose
    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.
    Depositor
    Ivo Lieberam
    Article
    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi
    Insert
    human PAX7
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Oct. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    204346-plasmid-map-sequence-id-410702

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  27. pVITRO1-102.1F10-IgE/λ

    Plasmid
    #50365
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgE/λ antibody isotype (102.1F10-IgE/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human epsilon heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50365-plasmid-map-sequence-id-367944

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  29. TFORF2408

    Plasmid
    #143370
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXF1 (FOXF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143370-plasmid-map-sequence-id-400341

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  36. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Use
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  37. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

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  44. HA-HA-AR-DBD-LBD

    Plasmid
    #171237
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171237-plasmid-map-sequence-id-336036

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  49. AiP12039 - pAAV-3xSP10ins-AiE0354h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2039)

    Plasmid
    #208401
    Purpose
    AiE0354h is an enhancer sequence designed to drive AAV-mediated transgene expression in Vip+ cortical interneurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Lee et al Science. 2023 Oct 13;382(6667):eadf6484.
    Insert
    SYFP2
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    eHGT_354h (enhancer)
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    208401-plasmid-map-sequence-id-416929

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  50. TFORF2959

    Plasmid
    #144435
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KAT2A (KAT2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144435-plasmid-map-sequence-id-407324

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Showing: 5751 - 5800 of 76289 results