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Showing: 451 - 500 of 8624 results

  7. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  18. pRL-SV40P

    Plasmid
    #27163
    Purpose
    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments
    Depositor
    Ron Prywes
    Article
    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702
    Insert
    SV40 promoter
    Use
    Luciferase
    Tags
    Renilla Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    27163-plasmid-map-sequence-id-419582

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  24. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105530-plasmid-map-sequence-id-454420

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  34. pGWB-nLUC

    Plasmid
    #174050
    Purpose
    Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay
    Depositor
    Alberto Macho
    Article
    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    N-terminal luciferase
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174050-plasmid-map-sequence-id-342879

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  38. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  49. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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Showing: 451 - 500 of 8624 results