19,180 results
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p3XFlag-CAPN2
Plasmid#60942PurposeExpresses calpain2 in mammalian cellsDepositorInsertCalpain 2 (CAPN2 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
eMscL G22S
Plasmid#107455PurposeeMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motifDepositorInsertbacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
UseAdeno-associated viralTagsKir2.1 ER export signal (FCYENEV) and tdTomato fl…ExpressionMammalianMutationBearing a glycine to serine substitution at posit…Promoterhuman synapsin 1Available sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pX330-SpCas9-NG
Plasmid#117919PurposeExpresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.DepositorInsertSpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6352 MSCV-CMV-CMV-Flag-HA-JMJD6
Plasmid#31358DepositorInsertJMJD6 (JMJD6 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET29b Tau Cys
Plasmid#108867PurposeExpresses human Tau with a mutated cysteineDepositorInsertTau (MAPT Human)
UseTagsExpressionBacterialMutationI260C C291A C322A to allow maleimide dye labellingPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Bxb1o_nlsFlag
Plasmid#119901PurposeExpresses Bxb1 integrase in mammalian cellsDepositorInsertMammalian codon optimized Bxb1 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA
Plasmid#51669PurposeCan be used to generate AAV virus that will express the FlpO recombinase gene in neurons from the synapsin promoter.DepositorHas ServiceAAV RetrogradeInsertFlpO recombinase gene
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV/SB11
Plasmid#26552DepositorInsertSleeping Beauty
UseTagsExpressionBacterialMutationMethionine 243 to Glutamine Threonine 136 to Arg…PromoterAvailable sinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC
Plasmid#125695PurposeGlobal labeling of inhibitory synapses (red fluorescence)DepositorInsertmScarlet-Gephyrin.FingR-IL2RGTC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP
Plasmid#75452PurposePlasmid for constitutive expression of gfpDepositorInsertgfp
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Integrin-Beta1-N-18
Plasmid#55064PurposeLocalization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertIntegrin-Beta1 (ITGB1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
amajLime chromoprotein
Plasmid#117843PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coliDepositorInsertpromoter, RBS, amajLime
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP4K4
Plasmid#23486DepositorInsertMAP4K4 (MAP4K4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma1-GFP2
Plasmid#140989PurposeEncodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma1-GFP2 (GNGT1 Human)
UseTagsGFP2 and GSAG linkerExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-LRP6c
Plasmid#42960PurposeExpresses optogenetically controlled LRP6 (C-terminal domain)DepositorInsertCry2PHR-mCherry-LRP6c
UseTagsExpressionMammalianMutationLPR6 cytoplasmic domain onlyPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]
Plasmid#40810DepositorInsertMAP2K1 (MAP2K1 Human)
UseTagsHAExpressionMammalianMutationdelta(31-51), S218E, S222DPromoterCMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG wildtype, catalytic active lipin 1
Plasmid#32005DepositorInsertlipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMV, SP6Available sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin1
Plasmid#124852PurposeMutagenesis of Grin1DepositorInsertGrin1 (Grin1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100853PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
NPY-td-Orange2
Plasmid#83497PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorInsertNPY (NPY Human)
UseTagstandem mOrange2ExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-GC3AI
Plasmid#78910PurposeIndicator of caspase-3-like protease activityDepositorInsertGC3AI
UseLentiviralTagsHA and MycExpressionMutationS320A, deletion of AA1-200PromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pU6-gRNA
Plasmid#53062PurposeTranscription of guide RNA (gRNA) in wheat cellsDepositorInsertwheat U6 promoter - gRNA
UseCRISPR; Plant expressionTagsExpressionMutationPromoterwheat U6Available sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-FLAG-KD LKB1
Plasmid#8593DepositorInsertLKB1 (STK11 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationK78I: lysine 78 mutated to isoleucine; kinase deadPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorInsertm6A-Tracer-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Sox9
Plasmid#41080DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetO + minimal CMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-EYFP
Plasmid#34582DepositorTypeEmpty backboneUseTags3xFLAG and EYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-N-I-N-CAAX
Plasmid#41686PurposeExpresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activationDepositorInsertDronpa145N-IntersectinDH-Dronpa145N-CAAX
UseTagsCAAX boxExpressionMammalianMutationK145N on DronpaPromoterCMVAvailable sinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
ACTN2-pEGFP
Plasmid#52669PurposeOverexpression of GFP-tagged α-actinin-2DepositorInsertactinin 2 (ACTN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Parkin
Plasmid#17613DepositorInsertParkin (PRKN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPalmitoyl-mTurquoise2
Plasmid#36209PurposeIn vivo visualization of the membrane (can be used for colocalization studies)DepositorInsertp63(1-29) (ARHGEF25 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTOX6
Plasmid#127451PurposeEncodes Tse2 toxin and amilCP chromoprotein. CAM-RDepositorInsertstse2 toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralTagsExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD152
Plasmid#125546Purposederivative of pFD116 with an optimized RBS for E. coliDepositorInsertoptimized RBS
UseCRISPRTagsExpressionMutationPromoteranhydrotetracycline Ptet promoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB
Plasmid#60522PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTagMBP
Plasmid#35050DepositorInsertSpyTagMBP
UseTagsHis6ExpressionBacterialMutationSimple peptide tag added to the N-terminus of MBP…PromoterT7 promoterAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
AmCyan-P2A-mCherry
Plasmid#45350DepositorInsertAmCyan
UseTagsmCherryExpressionMammalianMutationSV40 NLSPromoterCMVAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 S727A
Plasmid#12304DepositorInsertSTAT1 S727A (STAT1 Human)
UseTagseGFPExpressionMammalianMutationS727APromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
FFA3-Tango
Plasmid#66282PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertFFA3 (FFAR3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only
