169,365 results
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Plasmid#201208PurposeAAV vector for TRE(TRE3G)-driven smFP_Myc (Myc-tagged spaghetti monster) expressionDepositorInsertsmFP _Myc (from addgene #59757)
UseAAVExpressionBacterial and MammalianPromoterTRE3GAvailable SinceAug. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKII-HyPer7
Plasmid#238922PurposeMammalian expression of HyPer7 in excitatory neuronsDepositorInsertHyPer7
UseAAVExpressionMammalianPromoterCaMKIIalfaAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-36XMYC array
Plasmid#236380PurposeExpression of array of 36 crRNAs targeting MYCDepositorInsert36xMYC crRNA array (MYC Human)
UseCRISPR and LentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
NanoBRET Assay Vector, CRAF(FL)-HaloTag
Plasmid#236874PurposeExpress CRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertCRAF (RAF1 Human)
TagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nluc-KRAS(G12C)-cRAF(FL)-HaloTag BiBRET Vector
Plasmid#236868PurposeExpress NanoLuc(R)-KRAS(G12C)-cRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoterDepositorHas ServiceDNATagsHaloTag (R) and NanoLuc (R)ExpressionMammalianMutationG12CPromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn-NES-SomajRGECO1a
Plasmid#232842PurposeAAV transfer plasmid for Syn-mediated expression of soma-targeted jRGECO1aDepositorInsertNES-SomajRGECO1a
UseAAVTagsnuclear export sequence, 6xHis, T7, Xpress tagExpressionMammalianPromoterSynAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_AH
Plasmid#217602PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
TagsHisExpressionBacterialPromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-E690Kmut_ABCA3-GFP
Plasmid#188549PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion proteinDepositorInsertABCA3:GFP (ABCA3 Human)
UseLentiviralTagsGFPMutationamino acid E690->K690 (ABCA3 gene)PromoterEF1aLAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control-TP53-LRG-GFP
Plasmid#225874PurposeGuides targeting P53 and a control sequenceDepositorInsertControl and TP53 (TP53 Human)
UseCRISPR and LentiviralAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Control-RB1-LRG-GFP
Plasmid#225875PurposeGuides targeting RB1 and a control sequenceDepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-CDC20
Plasmid#221694PurposeMammalian expression of FLAG-tagged human CDC20DepositorInsertCDC20 (CDC20 Human)
ExpressionMammalianAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgCTG-CMV-GFP
Plasmid#216734PurposeAAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.DepositorArticleInsertsgCTG
UseAAV and CRISPRExpressionMammalianPromoterU6 and CMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-SpCas9D10A nickase
Plasmid#216736PurposeExpresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRExpressionMammalianMutationD10APromoterEF-1-alpha coreAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
A2C-∆N
Plasmid#217909PurposeExpresses bicone gas vesicles in E. coliDepositorInsertA2C-∆N
ExpressionBacterialPromoterT7Available SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CREBBP_ATG10
Plasmid#205795PurposeExpress mEGFP-tagged fusion protein, CREBBP_ATG10 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CREBBP_ZNF500
Plasmid#205797PurposeExpress mEGFP-tagged fusion protein, CREBBP_ZNF500 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFFV-mCh-GATA2
Plasmid#193071Purposeconstitutive expression of mCherry fused to human Gata2 in mammalian cellsDepositorInsertmCh-GATA2
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2319
Plasmid#142032PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GIT1-Flag/pBK
Plasmid#197313PurposePlasmid expressing an antigen targeting GIT1DepositorAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR3DX1-COMP5AP-AviTag-9xHis
Plasmid#157338PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR3DL3.1-COMP5AP-AviTag-9xHis
Plasmid#157299PurposeMammalian expression of cell-surface protein extracellular domain (isoform 1) fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR3DL3.2-COMP5AP-AviTag-9xHis
Plasmid#157300PurposeMammalian expression of cell-surface protein extracellular domain (isoform 2) fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DL1-COMP5AP-AviTag-9xHis
Plasmid#157232PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DL2-COMP5AP-AviTag-9xHis
Plasmid#157233PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DL3-COMP5AP-AviTag-9xHis
Plasmid#157234PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DS4-COMP5AP-AviTag-9xHis
Plasmid#157236PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DS3-COMP5AP-AviTag-9xHis
Plasmid#157237PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DS5-COMP5AP-AviTag-9xHis
Plasmid#157238PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DP1-COMP5AP-AviTag-9xHis
Plasmid#157227PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTnr#2/Cre
Plasmid#193245PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr geneDepositorAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCKDK gRNA (BRDN0001149253)
Plasmid#75505Purpose3rd generation lentiviral gRNA plasmid targeting human BCKDKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH753-CEN-RLuc/min346maxCFLuc
Plasmid#38219DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe first 346 codons of the original FLuc gene we…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pXR003_puroR
Plasmid#219819PurposehU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cAMPFIRE-H
Plasmid#182285PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.DepositorInsertcAMPFIRE-H (cAMP sensor)
ExpressionMammalianAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCasAb-apr
Plasmid#121998PurposeBacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumanniiDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-6h-GST-TEVp
Plasmid#172887PurposeCodon optimized (E. coli) TEVp fused with GST and his-tagged for purificationDepositorInsertTEV protease
TagsGST solubility tag and HHHHHHExpressionBacterialPromoterT7 promoterAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II NLS-MC-EGFP-tdTomato (pBET2)
Plasmid#240411PurposeThis plasmid facilitates the assessment of MMR proficiency in human living cellsDepositorInsertNLS-EGFP fusion protein
ExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
MCHR1-DuET
Plasmid#213337PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC G3BP1-GFP-GFP
Plasmid#119950PurposeLentiviral vector expressing G3BP1-GFP fusion proteinDepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Pam-FL
Plasmid#42570DepositorInserthuman MYCBP2 (MYCBP2 Human)
TagsMycExpressionMammalianMutationPam-FL clone lacks alternatively spliced exons 52…PromoterCMVAvailable SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-AmpR-MCS
Plasmid#188978PurposeFor red light-induced bacterial expression of gene of interest (AmpR)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC0569 (parts entry vector)
Plasmid#189966PurposeCloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestionDepositorInsertmNeonGreen
ExpressionMammalianPromoterBBa-J23100Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-9
Plasmid#208787PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP-T2A-Puro
Plasmid#72263PurposeExpress copGFP and puromycin resistance gene under EF1 promoterDepositorInsertcopGFP-T2A-Puro
UseLentiviralExpressionMammalianPromoterelongation factor-1Available SinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
Plasmid#195542PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertLenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP
Plasmid#226516PurposeMamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFPDepositorInsertTOMM20-SNAP-tag2-T2A-EGFP
ExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG
Plasmid#222852PurposeMammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 FcDepositorInsertAT118-L
TagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only