164,300 results

1. mc2 neo

   Plasmid

   #206214

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 5 pCOLI_A_STREP

   Plasmid

   #160147

   Purpose

   N-term 2xStrepII 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   2xStrepII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAcCas12nHS_RV6_PT1_empty

   Plasmid

   #203810

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6_PT1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA4-Vps34-Flag

   Plasmid

   #24398

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Vps34 (PIK3C3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbS5a-Opto-Cre-Vvd-2

   Plasmid

   #160400

   Purpose

   Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   Opto-Cre-Vvd-2
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188773

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETDuet1_CoiC_CoiSA

   Plasmid

   #208762

   Purpose

   Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiC
   CoiSA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   single nucleotide insertion (C70) at RBS of CoiC

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF0207

   Plasmid

   #141555

   Purpose

   Lentiviral vector for overexpressing the FOXP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXP2 (FOXP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/Myc-His(-)-HSulf-1

    Plasmid

    #13003

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSH87

    Plasmid

    #127705

    Purpose

    Pflp-13::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    flp-13

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSH88

    Plasmid

    #127703

    Purpose

    Pacr-2::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    acr-2

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST-DHFR F[3]-C (LEU2)

    Plasmid

    #177796

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[3]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-DHFR F[1,2]-C (TRP1)

    Plasmid

    #177795

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Use

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TAL2289

    Plasmid

    #36724

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCG-TALEN_Right (Fancg Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Ef1a-ConVERGD-eGFP-WPRE

    Plasmid

    #218745

    Purpose

    Provides AND intersectional (Cre+Flp) expression of eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-flpE-puro

    Plasmid

    #20733

    Purpose

    Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    FLPe recombinase
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 37 pCOLI_K_STREP

    Plasmid

    #160263

    Purpose

    N-term 2xStrepII 3C cleavable Kan resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACyR1

    Plasmid

    #172565

    Purpose

    Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    CymRAM-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCymRC

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAlbumin

    Plasmid

    #22037

    Depositor

    Didier Trono

    Insert

    partial sequence of human albumine
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LeGO-dKatushka2

    Plasmid

    #85214

    Purpose

    Lentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    dKatushka2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BsrGI site removed at 5' end, BsrGI site ins…

    Promoter

    SFFV

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA-KIF5A

    Plasmid

    #166958

    Purpose

    Expresses HA-tagged KIF5A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQLink-GST-4xCTD

    Plasmid

    #138471

    Purpose

    Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    4x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSH79

    Plasmid

    #127704

    Purpose

    Pttr-39::gfp1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    ttr-39

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MARIO/ pcDNA3

    Plasmid

    #124838

    Purpose

    mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

    Depositor

    Takeharu Nagai

    Article

    Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

    Insert

    MARIO
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSH86

    Plasmid

    #127706

    Purpose

    Pmyo-3::GFP1-10

    Depositor

    David M. Miller

    Article

    He et al Genetics. 2019 Apr 5. pii: genetics.119.

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HCT159_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

    Plasmid

    #233558

    Purpose

    Direct-expressing iCre297T AAV virus

    Depositor

    Tanya Daigle, Allen Institute for Brain Science

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    iCre297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBglobin

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR223_ETS1_WT

    Plasmid

    #82118

    Purpose

    Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCBC-DT2T3

    Plasmid

    #50591

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVQ CMV NanoV1-2a-EGFP ferritin

    Plasmid

    #79649

    Purpose

    Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

    Depositor

    Jeffrey Friedman

    Article

    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

    Inserts

    transient receptor potential vanillin 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSLQ14902

    Plasmid

    #239277

    Purpose

    Expresses gG2 for pertubing endogenous human GAPDH mRNA via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PspCas13b-hGAPDH-gRNA2 (GAPDH Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSLQ10622

    Plasmid

    #239274

    Purpose

    Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSLQ10550

    Plasmid

    #239269

    Purpose

    Expresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial membrane via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-MAVS(aa510-540) (MAVS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSLQ10552

    Plasmid

    #239271

    Purpose

    Expresses PYL1-G3BP1 for recruiting RNA to the stress granule via CRISPR-TO

    Depositor

    Stanley Qi

    Article

    Han et al Nature. 2025 Jul;643(8070):241-251. doi:

    Insert

    PYL1-BFP-G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTom20-mScarlet3-H (aka mYongHong)

    Plasmid

    #239412

    Purpose

    Expresses Tom20-mScarlet3-H in mammalian cells

    Depositor

    Zhifei Fu

    Article

    Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mScarlet3-H (aka mYongHong)

    Expression

    Mammalian

    Mutation

    M163H in mScarlet3

    Promoter

    CMV

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pNEU-KlacRS

    Plasmid

    #232155

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEvol-MmKlacRS1

    Plasmid

    #232157

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pENTR_sfGFP-TurboID

    Plasmid

    #240226

    Purpose

    Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    sfGFP-TurboID
    

    Use

    Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pStayGold-Tubulin

    Plasmid

    #229571

    Purpose

    Expresses StayGold-tubulin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    StayGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pENTR2b-EGFP

    Plasmid

    #215537

    Purpose

    Gateway entry vector with the EGFP fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. FLuc-N-HiBiT

    Plasmid

    #207117

    Purpose

    Bacterially expressed HiBiT-FLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    luciferin 4-monooxygenase
    

    Use

    Tags

    HiBiT Tag and His-tag with thrombin site

    Expression

    Bacterial

    Mutation

    Codon optmization for expression in BL21DE3

    Promoter

    T7 Promotor

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBIG1e-CDK12deltaRS/Cyclin K

    Plasmid

    #231744

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Use

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(delta104-380)

    Promoter

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTn7xTS_SpyCatcher:mKate2

    Plasmid

    #225188

    Purpose

    Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    SpyCatcher:mKate2
    

    Use

    Tags

    SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pZH61 pLIB-8His3C-spt4

    Plasmid

    #231649

    Purpose

    express S.cerevisiae spt4 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt4 (SPT4 Budding Yeast)
    

    Use

    Tags

    8His-HRV3C

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge