165,685 results

1. pGEX-4T-1 3xHA-MKK6-EE

   Plasmid

   #47579

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK6-EE (MAP2K6 Human)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCR1063

   Plasmid

   #111091

   Purpose

   Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   dCas9-KRAB
   

   Use

   Lentiviral

   Tags

   mCherry

   Available Since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Hisx6-Sirt6

   Plasmid

   #20277

   Depositor

   Leonard Guarente

   Article

   Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

   Insert

   Sirt6 (Sirt6 Mouse)
   

   Tags

   Hisx6

   Expression

   Bacterial

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-SLC17A8_STOP

   Plasmid

   #161127

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC17A8 (SLC17A8 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. mEmerald-DZIP1-N-18

   Plasmid

   #54070

   Purpose

   Localization: Centrosomes, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   DZIP1 (DZIP1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pBM16

   Plasmid

   #45344

   Depositor

   Brendan Cormack

   Article

   Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

   Type

   Empty backbone

   Use

   C. glabrata expression

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. NHA-Ago1

   Plasmid

   #21535

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   Ago1 (AGO1 Human)
   

   Tags

   NHA

   Expression

   Mammalian

   Available Since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

8. ITGB2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176983

   Purpose

   Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   ITGB2 (ITGB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_CSNK1E_WT

   Plasmid

   #82299

   Purpose

   Gateway Donor vector containing CSNK1E, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CSNK1E (CSNK1E Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDR-AmTrac

    Plasmid

    #47770

    Purpose

    expresses AmTrac in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac
    

    Tags

    mcpGFP

    Expression

    Yeast

    Promoter

    PMA

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZL Neo Myr Flag BEGAIN

    Plasmid

    #20567

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BEGAIN (BEGAIN Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. CMV-Rps7-meGFP

    Plasmid

    #241318

    Purpose

    Mouse Rps7 fused to meGFP for expression in mammalian cells

    Depositor

    Stavros Lomvardas

    Insert

    Rps7 (Rps7 Mouse)
    

    Tags

    meGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid

    #246438

    Purpose

    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 16 to Alanine, deleted amin…

    Promoter

    EF-1 alpha and U6

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid

    #246436

    Purpose

    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    

    Tags

    GFP N-terminal sequence (M1 to Q95), Hemi Intron …

    Expression

    Mammalian

    Mutation

    GFP N-terminal sequence M1 to Glutamine 95 append…

    Promoter

    CMV and U1a

    Available Since

    Oct. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMini-CMV-NLS-dead R-IscB-NLS_T2A_EGFP (Y67X)_LNMA intron

    Plasmid

    #246435

    Purpose

    Expresses inactive EGFP (Y67X) in mammalian cells for trans-splicing assessments. LMNA intron is inserted into EGFP CDS.

    Depositor

    Ailong Ke

    Article

    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

    Inserts

    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    EGFP
    

    Tags

    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 60 to Alanine, changed Hist…

    Promoter

    CMV

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNP3440 [pNP2922 - ISCro4 bRNA (TBL2, WT + DBL1, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247263

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 (WT, TBL2 + DBL1)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247261

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL4)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNP2389 [pNP2314 - ISCro4 Donor + ISCro4 Target-RTG-Matched]

    Plasmid

    #247251

    Purpose

    Measure ISCro4 activity via luciferase expression

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2314 - ISCro4_Donor, ISCro4_Target_RTG-Matched
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FEOX-PBCS-TBF2- MC2

    Plasmid

    #234406

    Purpose

    Tool for genetically encoded fluorescent biosensor of cellular iron microenvironment

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Sci Rep. 2025 Oct 21;15(1):36596. doi: 1

    Inserts

    FEOX1 CONTROL
    FEOX
    

    Use

    Synthetic Biology

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    EF1-alpha and hpgk

    Available Since

    Oct. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLPC-Myc-BirA

    Plasmid

    #234039

    Purpose

    Expresses Myc-His-BirA(R118G)

    Depositor

    Roderick O'Sullivan

    Article

    Garcia-Exposito et al Cell Rep. 2016 Nov 8;17(7):1858-1871. do

    Insert

    BirA
    

    Use

    Retroviral

    Tags

    Myc-6xHis

    Expression

    Mammalian

    Mutation

    R118G

    Available Since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5 HIS-Ub-K48R

    Plasmid

    #221368

    Purpose

    Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

    Depositor

    Aga Gambus

    Article

    Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

    Insert

    Ubiquitin
    

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Changed Lysine 48 to Arginine

    Promoter

    CMV

    Available Since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1(+)-Flag-SOD1-G93A

    Plasmid

    #235662

    Purpose

    Express human SOD1-G93A in mammalian cells

    Depositor

    Chen Seng Ng

    Article

    Hiew et al Commun Biol. 2025 Mar 2;8(1):347. doi: 1

    Insert

    SOD1-G93A (SOD1 Human)
    

    Tags

    Flag-tag at N-terminal

    Expression

    Mammalian

    Mutation

    changed Glycine 93 to alanine

    Promoter

    CMV promoter

    Available Since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPBbsr2-RaichuEV-Rac1HRasCT

    Plasmid

    #214818

    Purpose

    Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    RaichuEV-Rac1HRasCT
    

    Tags

    HRasCAAX

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCSIIbsr-Raichu-Rab5/PM

    Plasmid

    #214819

    Purpose

    Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFPc3 N-RAS

    Plasmid

    #236450

    Purpose

    transient overexpression of N-RAS in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    N-RAS (NRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFPc1 H-RAS

    Plasmid

    #236449

    Purpose

    transient overexpression of H-RAS in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    H-RAS (HRAS Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220731

    Purpose

    AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    May 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid

    #191212

    Purpose

    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    SARS-CoV-2 spike_WT (S SARS CoV-2)
    

    Tags

    HiBiT (high affinity small nanoluciferase fragmen…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA FRT to BioID-cDIC1-3xFlag

    Plasmid

    #232417

    Purpose

    Expresses human dynein subunit DIC1 in mammalian cells.

    Depositor

    Samara Reck-Peterson

    Article

    Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

    Insert

    Dynein Intermediate Chain 1 (DYNC1I1 Human)
    

    Tags

    BioID and Flag-3x

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXTK024 [Origin_RSF1010_PartType1]

    Plasmid

    #229261

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RSF1010 Origin
    

    Expression

    Bacterial

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ubi:ccdb + mcherry

    Plasmid

    #233303

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb + mcherry
    

    Use

    Gateway destination

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Ubi:ccdb-3xHA + mcherry

    Plasmid

    #233302

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of 3xHA tag paired with ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-3xHA + mcherry
    

    Use

    Gateway destination

    Tags

    3xHA

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Ubi:ccdb-eGFP + mcherry

    Plasmid

    #233300

    Purpose

    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and mCherry marker

    Depositor

    Thomas Baum

    Insert

    Ubi:ccdb-eGFP + mcherry
    

    Use

    Gateway destination

    Tags

    eGFP

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

    Plasmid

    #230360

    Purpose

    AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. sgPPP1R2

    Plasmid

    #227391

    Purpose

    Adhesion module, guide RNA recognizes PPP1R2

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    guide RNA targeting PPP1R2
    

    Use

    Lentiviral

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pACYC-yhaM-RFP

    Plasmid

    #207651

    Purpose

    Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    yhaM
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUdOm7.1

    Plasmid

    #210287

    Purpose

    Empty backbone with zeocin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMRS-dCas9

    Plasmid

    #220193

    Purpose

    mini-Tn7 dCas9 expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    promoterless

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBW-m4046749

    Plasmid

    #145629

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp11

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp11 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. Cytoplasmic PancACe

    Plasmid

    #215708

    Purpose

    Expresses acetyl-CoA biosensor in the cytoplasm

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Lentiviral

    Tags

    FLAG, HA, and cytoplasmic localization sequence

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. sortase A pentamutant (eSrtA) in pET29

    Plasmid

    #75144

    Purpose

    evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

    Depositor

    David Liu

    Article

    Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

    Insert

    sortase A pentamutant
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T. Deleted amino a…

    Promoter

    T7

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRFHUE-eGFP

    Plasmid

    #89469

    Purpose

    GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

    Depositor

    Luis González-Candelas

    Article

    Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

    Insert

    eGFP
    

    Use

    Binary plasmid for atmt of filamentous fungi

    Promoter

    A. nidulans gpdA

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCMV R-CEPIA1er

    Plasmid

    #58216

    Purpose

    Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    R-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available Since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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48. pRL056 pDESTattB_exorh:EGFP

    Plasmid

    #213510

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCMV2-SEP-GluA1 (M1)

    Plasmid

    #64942

    Purpose

    mammalian expression of rat GluA1

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    GluA1 (Gria1 Rat)
    

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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