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175,573 results

Showing: 1451 - 1500 of 175573 results

  3. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available Since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  4. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Insert
    Clover-mRuby2 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49089-plasmid-map-sequence-id-114654

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  10. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-512037

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  21. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    human Synapsin1
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  27. pLV-Azurite

    Plasmid
    #36086
    Purpose
    3rd generation lentiviral plasmid
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36086-plasmid-map-sequence-id-244564

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  30. pEZYmyc-His

    Plasmid
    #18701
    Purpose
    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Tags
    Myc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18701-plasmid-map-sequence-id-181026

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  33. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Expression
    Mammalian
    Available Since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  42. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  44. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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  46. pCAG-NLS-HA-Dre

    Plasmid
    #51272
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Dre
    Tags
    NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51272-plasmid-map-sequence-id-334322

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Showing: 1451 - 1500 of 175573 results