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Showing: 2601 - 2650 of 4061 results

  9. pcDNA3 mCherry LIC cloning vector (6B)

    Plasmid
    #30125
    Purpose
    Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    30125-plasmid-map-sequence-id-174010

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  16. pCW57.1-FOXM1b

    Plasmid
    #68811
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1b (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68811-plasmid-map-sequence-id-419548

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  17. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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  25. pAAV-hSyn-DIO-mCherry

    Plasmid
    #50459
    Purpose
    Double floxed mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50459-plasmid-map-sequence-id-171068

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  26. pTP1112

    Plasmid
    #104158
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104158-plasmid-map-sequence-id-200058

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  34. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

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  36. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  39. pDest-527

    Plasmid
    #11518
    Purpose
    Gateway destination vector for bacterial expression; His tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11518-plasmid-map-sequence-id-181065

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  42. pPGK-Cre-bpA

    Plasmid
    #11543
    Purpose
    Expresses Cre recombinase from mouse PGK promoter in mammalian cells.
    Depositor
    Klaus Rajewsky
    Insert
    Phosphoglycerate kinase 1 promoter
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse PGK
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11543-plasmid-map-sequence-id-6971

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  43. pBABE puro H-Ras V12

    Plasmid
    #9051
    Purpose
    Retroviral expression of a constitutively active form of H-Ras
    Depositor
    William Hahn
    Insert
    Hras V12 (HRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    G12V, constitutively active
    Promoter
    Available since
    Nov. 2, 2005
    Availability
    Academic Institutions and Nonprofits only
    9051-plasmid-map-sequence-id-170484

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  46. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Article
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Mutation
    Promoter
    p10 promoter
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13763-plasmid-map-sequence-id-364424

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  47. pCW57-GFP-2A-MCS

    Plasmid
    #71783
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71783-plasmid-map-sequence-id-197192

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  50. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid
    #141184
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has Service
    Cloning Grade DNA
    Insert
    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    Use
    Tags
    Signal peptide from influenza HA and Superfolder …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141184-plasmid-map-sequence-id-306284

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Showing: 2601 - 2650 of 4061 results