4,117 results

1. pB18cmBFPAzurite

   Plasmid

   #14034

   Depositor

   Patrick Daugherty

   Article

   Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

   Insert

   Azurite
   

   Use

   Yeast/bacteria optimized codons

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pARF6(T27N)-CFP

   Plasmid

   #11386

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 6 (ARF6 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Changed Threonine 27 to Asparagine.

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-CaMPARI W391F V398L

   Plasmid

   #60787

   Purpose

   Expresses CaMPARI W391F V398L in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI W391F V398L
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BcatMutS33/S37.T41/S45

   Plasmid

   #24204

   Depositor

   David Rimm

   Insert

   beta catenin (CTNNB1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S33A, S37A, T41A,S45A

   Promoter

   CMV

   Available since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPRIME-TET-GFP-FF3

   Plasmid

   #11662

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-HA GST RagB wt

   Plasmid

   #19301

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Use

   Tags

   GST and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hTLR3 flag

   Plasmid

   #13084

   Depositor

   Ruslan Medzhitov

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Amino acids 1-17 have been replaced by mammalian …

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MDH1-PGK-GFP_2.0

   Plasmid

   #11375

   Purpose

   Retroviral construct for miRNA expression

   Depositor

   Chang-Zheng Chen

   Article

   Chen et al Science. 2004 Jan 2. 303(5654):83-6.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-myc-LC3 (Atg8)

   Plasmid

   #24919

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPhiC31o

    Plasmid

    #13794

    Purpose

    Expression of mouse codon-optimized PhiC31 recombinase

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

    Insert

    PhiC31o
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-HA GST RagB 54L

    Plasmid

    #19302

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    T54L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pDONR223-KDR

    Plasmid

    #23925

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    KDR (KDR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V297I

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-HA-VCPIP1

    Plasmid

    #22592

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    VCPIP1 (VCPIP1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E[beta]P

    Plasmid

    #24313

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-HA-USP42

    Plasmid

    #22603

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP42 (USP42 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX404 Campylobacter jejuni Cas9

    Plasmid

    #68338

    Purpose

    Campylobacter jejuni Cas9

    Depositor

    Feng Zhang

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    HA-NLS and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSicoR PGK puro

    Plasmid

    #12084

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIREShyg-PA-GFP-alpha Tubulin

    Plasmid

    #12296

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBFRE-luc

    Plasmid

    #26897

    Depositor

    Nicholas Gaiano

    Article

    Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

    Insert

    CBFRE
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

21. Flag-HA-OTUD7B

    Plasmid

    #22550

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD7B (OTUD7B Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV.ATMi

    Plasmid

    #14542

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    ATM shRNA (ATM Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-HA-CYLD

    Plasmid

    #22544

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    CYLD (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQC NLS mCherry IX

    Plasmid

    #37354

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    NLS-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pST1374-N-NLS-flag-linker-Cas9-D10A

    Plasmid

    #51130

    Purpose

    Cas9 nickase optimized for nuclear import.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Insert

    Cas9 nickase
    

    Use

    CRISPR

    Tags

    Flag and NLS

    Expression

    Mammalian

    Mutation

    D10A (nickase mutant)

    Promoter

    CMV

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-rab9 DN

    Plasmid

    #12664

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    changed serine 21 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQCXI Neo DsRed-LC3-GFP

    Plasmid

    #31183

    Depositor

    David Sabatini

    Article

    Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

    Insert

    DsRed-LC3-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pAAV-fSST-RFP

    Plasmid

    #22913

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    fugu somatostatin promoter driving DsRed 2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBiFC-bFOSDelataZipVC155

    Plasmid

    #22014

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bFosDelataZIPVC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of residues 179-193 in bFos(118-211)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWZL Blast Twist ER

    Plasmid

    #18799

    Depositor

    Bob Weinberg

    Article

    Mani et al Cell. 2008 May 16. 133(4):704-15.

    Insert

    twist (Twist1 Mouse)
    

    Use

    Retroviral

    Tags

    ER hormone binding domain

    Expression

    Mammalian

    Mutation

    G525R mutation in the ER. The presence of the G52…

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEMTEZ-TeTxLC

    Plasmid

    #32640

    Depositor

    Joseph Gogos, C. Ron Yu, Richard Axel

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Tetanus toxin light chain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. aMHC-eGFP-Rex-Neo

    Plasmid

    #21229

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSicoR p53

    Plasmid

    #12090

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Insert

    p53 shRNA (Trp53 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    EGFP is expressed from this plasmid as a marker, …

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. gRNA_GFP-T2

    Plasmid

    #41820

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mouse Drp1(K38A)

    Plasmid

    #26049

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K38A GTPase mutant

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

37. aP2-luciferase

    Plasmid

    #8858

    Depositor

    Bruce Spiegelman

    Article

    Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

    Insert

    aP2 enhancer / promoter (Fabp4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase reporter

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFLAG/HA-DGCR8

    Plasmid

    #10921

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pCS2P+

    Plasmid

    #17095

    Depositor

    Marc Kirschner

    Type

    Empty backbone

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pmCherry-ATG5-K130R

    Plasmid

    #13096

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5-K130R (Atg5 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K130R

    Promoter

    Available since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGL2Basic-EcadK1

    Plasmid

    #19290

    Depositor

    Eric Fearon

    Article

    Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

    Insert

    E-cadherin
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    E-cad promoter sequences from -108 to +125

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. piRFP682-N1

    Plasmid

    #45459

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP682
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. miR-146a

    Plasmid

    #15092

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    pre-miR-146a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Cas9m4-VP64

    Plasmid

    #47319

    Purpose

    Cas9m4 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m4-VP64
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCEP4YPet-MAMM

    Plasmid

    #14032

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    YPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian optimized YPet

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. CFP-FKBP (CF)

    Plasmid

    #20160

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    CFP-FKBP (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. FUW-tetO-lox-hKLF4

    Plasmid

    #20727

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMXs-Sall4

    Plasmid

    #13361

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    sal-like 4 (Drosophila) (Sall4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pENTR4-CLIPf (w877-2)

    Plasmid

    #29650

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    CLIPf

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge