9,048 results

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pcDNA3 HA Arf1

Plasmid

#10830

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 (ARF1 Bovine)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pQE60-RRF-His

Plasmid

#124132

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Ribosome recycling factor (frr Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMT413

Plasmid

#8606

Depositor

Robert Hartley

Article

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
ArcLight-A242

Plasmid

#36857

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

semian CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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Luc.Cre empty vector

Plasmid

#20905

Depositor

Tyler Jacks

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Purpose

Codon optimized Cas9_SV40 NLS with intron

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-OsU6b

Plasmid

#66196

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

OsU6b

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChR2-mCherry

Plasmid

#58892

Purpose

AAV-mediated expression of ChR2-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has Service

AAV5

Insert

ChR2-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-QF

Plasmid

#24338

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVUHshp53

Plasmid

#11653

Purpose

3rd generation lentiviral transfer vector expressing shp53 under an hUbiquitin promoter.

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

GFP, shRNA against TP53 (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Promoter

-1748CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207 SARS-CoV-2 E

Plasmid

#141273

Purpose

Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

E (E SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA HA HP1 alpha

Plasmid

#24078

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 alpha (CBX5 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRsetB-His7tag-Peredox-mCherry

Plasmid

#32382

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

His7-Peredox-mCherry

Tags

His7tag

Expression

Bacterial

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-hEAAT2

Plasmid

#32814

Depositor

Susan Amara

Insert

hEAAT2 (SLC1A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-FUW-sox2

Plasmid

#20326

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

sox2 (Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 (FL)

Plasmid

#24388

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-puro LEF1

Plasmid

#27023

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

LEF1 (LEF1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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SpyTag-β-Lactamase-SpyCatcher

Plasmid

#52656

Purpose

Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

Depositor

Mark Howarth

Article

Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

Insert

TEM-1 β-Lactamase

Tags

SpyCatcher and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Keratin 8 (pcDNA3)

Plasmid

#18063

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-UBR5 ∆HECT

Plasmid

#52051

Purpose

CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion

Depositor

Darren Saunders

Article

Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

Insert

UBR5 ∆HECT (UBR5 Human)

Tags

GFP

Expression

Mammalian

Mutation

C2769A

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

Plasmid

#32149

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

FRT> STOP STOP FRT>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAP

Plasmid

#11558

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAP (XIAP Human)

Tags

Flag

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-H_C1

Plasmid

#85043

Purpose

Mammalian expression of mScarlet-H

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

mScarlet-H

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-FLAG LIP

Plasmid

#15737

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM2991

Plasmid

#40248

Depositor

Susan Golden

Article

Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

Insert

One Step Cloneing Vector for Overexpression

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-tTRKRAB-red

Plasmid

#12250

Depositor

Didier Trono

Article

Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

Insert

TetR-KRAB fusion and dsRED

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Mutation

dsRed added to backbone.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pT-CA-GFP-IRES-mLumin

Plasmid

#32749

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Inserts

CA-GFP
mLumin

Expression

Mammalian

Mutation

S158A and S65T

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDESTmycDICER

Plasmid

#19873

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

dicer 1, ribonuclease type III (DICER1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 human cofilin S3A

Plasmid

#50860

Purpose

expresses constituvely active cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S3A constitutively active

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE Dlg4-GFP KI

Plasmid

#131477

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
VAMP2-pHuji

Plasmid

#105289

Purpose

VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

Depositor

Justin Taraska

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

VAMP2 (Vamp2 Rat)

Tags

pHuji

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C5V

Plasmid

#26394

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-5-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin A (CCNA2 Human)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-LYN

Plasmid

#23905

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LYN (LYN Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Article

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP(N3)-PP1beta

Plasmid

#44223

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1B (PPP1CB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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9,048 results