165,511 results

1. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ10875

   Plasmid

   #183963

   Purpose

   CBA-hyperdCas12a-HA-KRAB-mCherry

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, KRAB, and mCherry

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CBA

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ10878

   Plasmid

   #183964

   Purpose

   CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FRT-EF1a-TVA-2A-N2cG

   Plasmid

   #172361

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-Tspan4

   Plasmid

   #210323

   Purpose

   Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

   Depositor

   Li Yu

   Article

   Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

   Insert

   Tetraspanin 4 (Tspan4 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-FFSS-Cyclin D1

   Plasmid

   #215151

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3puro-mGFP

   Plasmid

   #225446

   Purpose

   Express mEGFP-fusion protein of interest

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Type

   Empty backbone

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX.TRE3G.AdProt

    Plasmid

    #131687

    Purpose

    A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus Protease
    

    Use

    Lentiviral

    Promoter

    TRE3GV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-nEF-Con/Fon TVA-mCherry

    Plasmid

    #131779

    Purpose

    INTRSECT 2.0

    Depositor

    Karl Deisseroth

    Article

    Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

    Insert

    TVA-mCherry
    

    Use

    AAV; Intrsect

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    nEF

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD864-LacZ

    Plasmid

    #109380

    Purpose

    Rhamnose inducible LacZ expression. Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pRHAM-LacZ
    

    Expression

    Bacterial

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpyDock

    Plasmid

    #124618

    Purpose

    Expresses SpyDock to bind SpyTag non-covalently for affinity purification

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyDock
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM-AAV-mSncg-Cre

    Plasmid

    #153207

    Purpose

    Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    mSncg

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid

    #159918

    Purpose

    Mutagenesis of Drd2

    Depositor

    Larry Zweifel

    Insert

    Drd2 (Drd2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. L_NLS 41kDa

    Plasmid

    #201342

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-2PrA
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

    Plasmid

    #140086

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE

    Plasmid

    #202199

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Has Service

    AAV1 and AAV5

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-SP6rpol

    Plasmid

    #165516

    Purpose

    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    SP6 RNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pDisplay-SNAPtag

    Plasmid

    #205094

    Purpose

    Display SNAPtag on the surface of mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    SNAPtag
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDisplay-GRAB_r5-HT1.0

    Plasmid

    #208713

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ADRB3-DuET

    Plasmid

    #213182

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADRB3 (ADRB3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. lentiCRISPR v2 sgKEAP1-3

    Plasmid

    #186837

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiCRISPR v2 sgKEAP1-2

    Plasmid

    #186459

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-EF1A-CD19.28H.28TM.28.BB.3z

    Plasmid

    #200680

    Purpose

    Modular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. IMPT-10314:GABBR2

    Plasmid

    #157851

    Purpose

    HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

    Depositor

    Vadim Cherezov, Raymond Stevens

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABBR2 (GABBR2 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-link-yomRuby2-SpHis5

    Plasmid

    #44858

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Expression

    Yeast

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLL5.0-MRLC-DD-GFP

    Plasmid

    #187260

    Purpose

    Lentiviral expression of myosin regulatory light chain-DD mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    T18D, S19D

    Promoter

    5' LTR

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDIO-DSE-mCherry-PSE-MCS

    Plasmid

    #129669

    Purpose

    Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

    Depositor

    Beatriz Rico

    Article

    Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry

    Promoter

    EF1a

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV-SV40-puro-EF1a-HA-NLGN1-bc

    Plasmid

    #200167

    Purpose

    Expresses HA-tagged human NLGN1

    Depositor

    Paul Blainey

    Article

    Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

    Insert

    Neuroligin-1 (NLGN1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHIP_RNA1

    Plasmid

    #61259

    Purpose

    Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA1
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDL-hu FceRI alpha

    Plasmid

    #8365

    Depositor

    Jean Kinet

    Article

    Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

    Insert

    human Fc epsilon RI alpha cDNA (FCER1A Human)
    

    Expression

    Mammalian

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

    Plasmid

    #103849

    Purpose

    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    CMV

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hSyn-GRAB_CRF1.0

    Plasmid

    #208658

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-TetOne-Puro-MDM4

    Plasmid

    #195140

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMOD_A0101

    Plasmid

    #90998

    Purpose

    Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRANS_221

    Plasmid

    #91115

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mEGFP-8xbiRhoBAST

    Plasmid

    #196351

    Purpose

    Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-8xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLL5.0-MRLC-WT-GFP

    Plasmid

    #187258

    Purpose

    Lentiviral expression of myosin regulatory light chain-WT

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    5' LTR

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCS2+8CmCherry

    Plasmid

    #34935

    Depositor

    Amro Hamdoun

    Article

    Gokirmak et al J Biol Chem. 2012 Nov 2.

    Type

    Empty backbone

    Use

    Sea urchin, zebrafish, xenopus

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRRL.SP146.gp47.eGFP

    Plasmid

    #30475

    Depositor

    Didier Trono

    Article

    Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

    Insert

    SP146/gp47
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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47. pUF-Cas9-pre-sgRNA

    Plasmid

    #137845

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pRK5-myc-Rac1-T17N

    Plasmid

    #12984

    Depositor

    Gary Bokoch

    Insert

    Rac1 DN (RAC1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONR223_CHD2_WT

    Plasmid

    #81768

    Purpose

    Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CHD2 (CHD2 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-hSyn1-tdTomatof

    Plasmid

    #104060

    Purpose

    Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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