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162,408 results

Showing: 8851 - 8900 of 162408 results

  1. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    MESV 5' LTR
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  17. pKDR-EF1a

    Plasmid
    #183567
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Unattenuated
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183567-plasmid-map-sequence-id-370308

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  29. pUltra-5HTP

    Plasmid
    #178042
    Purpose
    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T107C, P254T, C255A
    Promoter
    Available since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178042-plasmid-map-sequence-id-352270

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  30. ICAM2-bio-His

    Plasmid
    #51758
    Purpose
    Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ICAM2 (ICAM2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51758-plasmid-map-sequence-id-79347

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  32. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a and U6
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  41. SnapTag-Binder

    Plasmid
    #179142
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179142-plasmid-map-sequence-id-353405

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  45. MC177VSG221RNAi

    Plasmid
    #59730
    Purpose
    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.
    Depositor
    Gloria Rudenko
    Article
    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10
    Insert
    VSG221 RNAi fragment
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    opposing T7 promoters
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59730-plasmid-map-sequence-id-93746

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  46. pRGE32

    Plasmid
    #63159
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63159-plasmid-map-sequence-id-105873

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  50. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Use
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162280-plasmid-map-sequence-id-334621

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Showing: 8851 - 8900 of 162408 results