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9,128 results

Showing: 1251 - 1300 of 9128 results

  1. pQUAST-attB

    Plasmid
    #104880
    Purpose
    For insertion of 5xQUAS-geneX transgenes using PhiC31 integrase system
    Depositor
    Christopher Potter
    Type
    Empty backbone
    Expression
    Insect
    Available Since
    March 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    104880-plasmid-map-sequence-id-204757

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  6. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  11. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available Since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  17. pLKO.1 mCherry

    Plasmid
    #128073
    Purpose
    puro exchanged to mCherry
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128073-plasmid-map-sequence-id-255273

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  19. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  20. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  26. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  32. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  48. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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Showing: 1251 - 1300 of 9128 results