-
Plasmid#193830PurposeCMV overexpression of ChemoL-CaM in mammalian cell linesDepositorInsertChemoL-CaM
UseTagsExpressionMammalianMutationEGFP:A206K, E299D; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Full Length
Plasmid#159142PurposeExpresssion and protein purification of full length RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
Plasmid#175563PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1DepositorInsertGOXL1
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Bet1
Plasmid#120243PurposeExpresses V5-tagged Bet1 in lentiviral vectorDepositorInsertBet1 (Bet1 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #1
Plasmid#68876PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex5
Plasmid#85535PurposeInducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporterDepositorInsertmu p53
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTK066
Plasmid#65173PurposeEncodes tTDH1 as a Type 4b part to be used in the Dueber YTK systemDepositorInserttTDH1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta N53
Plasmid#19950DepositorInsertcullin 1 delta N53 (CUL1 Human)
UseTagsHAExpressionMammalianMutationdeletion of 53 residues from the NH2-terminus(de…PromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-p38 β/MAPK11 5'-UTR
Plasmid#115357Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNADepositorInsertp38 β/MAPK11 5'-UTR (MAPK11 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta C22
Plasmid#19942DepositorInsertcullin 1 delta C22 (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of 22 amino acids from the C terminusPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
PKD1 gRNA (BRDN0001149263)
Plasmid#76843Purpose3rd generation lentiviral gRNA plasmid targeting human PKD1DepositorInsertPKD1 (PKD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCS-shCHD5 #2
Plasmid#68877PurposeCHD5 shRNA expressed from Adeno-associated viral (AAV) vectorDepositorInsertCHD5
UseAAV and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BMPS C231S-6xHis
Plasmid#204834PurposeMammalian expression of recombinant BMPS C231SDepositorInsertCLN5 (CLN5 Human)
UseTags6xHisExpressionMammalianMutationC231SPromoterCMVAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BMPS WT-6xHis
Plasmid#204833PurposeMammalian expression of recombinant BMPS WTDepositorInsertCLN5 (CLN5 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-fDIO-mCherry-IRES-WGA-Cre
Plasmid#232905PurposeFlp-dependent anterograde transsynaptic tracerDepositorInsertSyn-fDIO-mCherry-IRES-WGA-Cre
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEB multi-Hyg Human collagen type IV alpha 4 chain (COL4A4)
Plasmid#229771Purpose3xFlag-tagged human Col4A4 to be used with N- or C-tagged LgBiT Col4A5 and SmBiT Col4A3 in Nanobit assay systemDepositorInsertHuman collagen type IV alpha 4 chain (COL4A4) (COL4A4 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
iGFP-gamma-actin
Plasmid#231554PurposeMammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFPDepositorInsertACTG1 (ACTG1 Human)
UseTagsmsfGFP (intramolecular)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-HIF2a dPA (P405A, P531A)
Plasmid#232954PurposeStably express a constitutively stable version of HIF2a (HIF2a dPA)DepositorInsertHIF2a (EPAS1 Human)
UseLentiviralTagsExpressionMammalianMutationP405A, P531APromoterEF1aAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-gfaABC1D-PinkyCaMP
Plasmid#232859PurposeAn AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoterDepositorInsertPinkyCaMP
UseAAVTagsExpressionMammalianMutationPromotergfaABC1DAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPMS-1852
Plasmid#232287PurposePhage DisplayDepositorInsertFn3 domain #10
UsePhage display vectorTagsPreScission Cleavage, HAExpressionMutationD7KPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)
Plasmid#229767PurposeHuman Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay systemDepositorInsertHuman collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
UseLuciferaseTagsSmBiTExpressionMutationSee Depositor CommentsPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc
Plasmid#229502PurposeMyc 5' untranslated region firefly luciferase reporter.DepositorInsertMyc 5'UTR
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCJ1021
Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[METUTRwt/luc2/minP]
Plasmid#226458Purposewt MET 5'UTR upstream FLucDepositorInsertMET 5'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1/luc2/minP]
Plasmid#226459PurposeMET 5'UTR (uORF1 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF2/luc2/minP]
Plasmid#226460PurposeMET 5'UTR (uORF2 mutated) upstream FLucDepositorInsertMET 5'UTR uORF2 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23[uORF1,2/luc2/minP]
Plasmid#226461PurposeMET 5'UTR (uORF1 and uORF2 mutated) upstream FLucDepositorInsertMET 5'UTR uORF1 uORF2 mutated
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HyPer7RgDAAO
Plasmid#217746PurposeDonor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.DepositorInsertHyPer7 D amino acid oxidase
UseTagsNuclear export signalExpressionMammalianMutationFused DAAO to the C-terminus of HyPer7 using a Gl…PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12253 - pAAV-AiE0458m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2253)
Plasmid#223976PurposeAiE0458m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14533-pAAV-DLX2.0-minBG-DIO-SYFP2-WPRE-HGHpA (Alias: CN4533)
Plasmid#222938PurposeCre-dependent expression of SYFP2 under the DLX2.0 enhancerDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)
Plasmid#222939PurposeFlpO recombinase expression from the DLX2.0 enhancerDepositorInsertFlpO (flp Synthetic)
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGExpressionMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TfCut2-S121P-D174S-D204P
Plasmid#218695PurposeEncodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expressionDepositorInsertTfCut2
UseTags10xHisExpressionBacterialMutationS121P-D174S-D204PPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-iLACCO1.1
Plasmid#218822PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinityDepositorInsertiLACCO1.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-BC-TurboID-GOI
Plasmid#209390PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID
Plasmid#209388PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_24 [pBBR1-UP ORI]
Plasmid#198937PurposeLevel 0 partDepositorInsertpBBR1-UP ORI
UseSynthetic BiologyTagsExpressionMutationS100L (C299T)PromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis
Plasmid#157239PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertKIR2DS1 (KIR2DS1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-HA
Plasmid#162103PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-ProtC-HA
UseLentiviralTagsVSVg-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-FLAG
Plasmid#162106PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to VSVg-HA-FLAG
UseLentiviralTagsVSVg-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1
Plasmid#162111PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
UseLentiviralTagsStrepTagII-ProtC-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-AU1
Plasmid#162116PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to ProtC-HA-AU1
UseLentiviralTagsProtC-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-AU1
Plasmid#162087PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-HA-AU1
UseLentiviralTagsVSVg-HA-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG
Plasmid#162090PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
UseLentiviralTagsStrepTagII-ProtC-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-FLAG
Plasmid#162095PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-HA-FLAG
UseLentiviralTagsProtC-HA-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1
Plasmid#162097PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-FLAG-AU1
UseLentiviralTagsProtC-FLAG-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only