165,255 results

1. pMXs-3XMyc-EGFP-OMP25

   Plasmid

   #83355

   Purpose

   For tagging mitochondria with Myc epitopes (Control-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XMyc-EGFP-OMP25
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

   Plasmid

   #135391

   Purpose

   Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

   Depositor

   Fan Wang

   Insert

   TeLC-P2A-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. anti-FLAG M2 light chain

   Plasmid

   #175358

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 light chain

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 light chain
   

   Tags

   N-terminal secretion signal peptide (MEAPAQLLFLLL…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Tags

   FLAG peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His-lsMSP1D1

   Plasmid

   #240151

   Purpose

   Expression of the MSP 'His-lsMSP1D1'

   Depositor

   Lise Arleth

   Article

   Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

   Insert

   His-lsMSP1D1
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB_EF1a_tNGFR_ZeoR

   Plasmid

   #212648

   Purpose

   piggyBac vector to express tNGFR with zeocin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pscAAV-GFP

   Plasmid

   #32396

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Promoter

    none

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Promoter

    none

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEcCas-2.0

    Plasmid

    #190697

    Purpose

    Constitutive expressing of Cas9 and inducible expression of lambda Red

    Depositor

    Sheng Yang

    Article

    Li et al ACS Synth Biol. 2023 Mar 3. doi: 10.1021

    Insert

    Cas9 and Lamda Red system
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-MmPeg10rc4

    Plasmid

    #174858

    Purpose

    Expresses recoded mouse Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Mus musculus PEG10 (Peg10 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOG44_FLP_recombinase (pEHA1338)

    Plasmid

    #209087

    Purpose

    FLP recombinase for FRT-mediated integration

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Insert

    FLP recombinase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-flex-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234441

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Has Service

    AAV1

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_BE4max_P2A_GFP

    Plasmid

    #112099

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max_P2A_GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti- V6.3 Ultra

    Plasmid

    #106172

    Purpose

    lentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectable

    Depositor

    Ewa Snaar-Jagalska

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    T2A, P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rab27A

    Plasmid

    #89237

    Purpose

    Expression of GFP-tagged human Rab27A in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    human Rab27A (RAB7A Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-CMV-Puro-miniAAVR-FLAG

    Plasmid

    #166717

    Purpose

    Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    miniAAVR-FLAG
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Only expressing PKD1-3 of ectodomain

    Promoter

    CMV

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-GfaABC1D-GRAB_ATP1.0

    Plasmid

    #167579

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR_Gal4UAS_tBFP_PGK_mCherry

    Plasmid

    #79130

    Purpose

    Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    tBFP
    

    Use

    Lentiviral

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiGuide-puro-NLS-mCherry

    Plasmid

    #185474

    Purpose

    Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHR_TRE3G_EGFP_WPRE

    Plasmid

    #231954

    Purpose

    Lentiviral vector for inducible expression of the green fluorescent protein EGFP (TRE3G promoter)

    Depositor

    Leonardo Morsut

    Insert

    EGFP
    

    Use

    Lentiviral

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cre Reporter

    Plasmid

    #62732

    Purpose

    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    LoxP-DsRED-STOP-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    LoxP sites

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yoEGFP-CaURA3

    Plasmid

    #44872

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Expression

    Yeast

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MS2_GFP

    Plasmid

    #61764

    Purpose

    Expresses MS2 coat protein fused to GFP

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    MS2 coat protein fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDestTol2CG2-U6:gRNA

    Plasmid

    #63156

    Purpose

    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPGK-T7/2-CD44v8-10 (fl)

    Plasmid

    #137823

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v8-10 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAXY0002

    Plasmid

    #179835

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Type

    Empty backbone

    Use

    Binary vector for plant transformation

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CMV-mCherry-(F30-2xPepper)10

    Plasmid

    #129401

    Purpose

    mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    mCherry-(F30-2xPepper)10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCHA-VRC01-scFv

    Plasmid

    #44874

    Depositor

    Dane Wittrup

    Article

    Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

    Insert

    VRC01 scFv
    

    Tags

    HA and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. UAS:mCherry

    Plasmid

    #170994

    Purpose

    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    UAS:mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Synthetic 14x UAS:Gal4

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LentiGuide-TagRFP-2A-BSD

    Plasmid

    #167930

    Purpose

    Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSIQmate

    Plasmid

    #216240

    Purpose

    All-in One IQ-Cumate transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSAB35

    Plasmid

    #175787

    Purpose

    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

    Depositor

    John Rubinstein

    Article

    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

    Insert

    SidK (1-278)
    

    Tags

    3xFLAG and 6xHis TEV

    Expression

    Bacterial

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge