Skip to main content
Addgene
Showing: 2101 - 2150 of 159111 results
  1. pD649-HAsp-NEGR1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156728
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Insert
    NEGR1 (NEGR1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. TFORF0723

    Plasmid
    #142226
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    NFKB1 (NFKB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. TFORF2095

    Plasmid
    #141918
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    ST18 (ST18 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pSL1-E2C

    Plasmid
    #180421
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes an E2 Crimson promoter.
    Depositor
    Insert
    E2 Crimson
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pCfB6633

    Plasmid
    #106162
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance
    Depositor
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. TFORF1207

    Plasmid
    #144089
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    CASZ1 (CASZ1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. OTUD5

    Plasmid
    #28270
    Depositor
    Insert
    OTUD5 (OTUD5 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    OTU DOMAIN (residues 172-339).
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pcDNA3.1-3xHA-mASL

    Plasmid
    #34577
    Purpose
    NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.
    Depositor
    Insert
    ASL (Asl Mouse)
    Use
    Tags
    1xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only
  9. pCIFR-3

    Plasmid
    #233295
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Insert
    msfGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    p14G
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pFNC-3

    Plasmid
    #233296
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.
    Depositor
    Inserts
    BxbI phage integrase
    sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PEM7 and unknown
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pPLP4HS-mCf

    Plasmid
    #229988
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Strep-tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pCMV-PuroR-2A-MLH1-SB

    Plasmid
    #228871
    Purpose
    Plasmid for MLH1-SB overexpression
    Depositor
    Insert
    PuroR-2A-MLH1-SB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. eiABE

    Plasmid
    #227144
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
    Depositor
    Insert
    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    Use
    Tags
    Expression
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. AAV-sCAG-eGFP-RAB11

    Plasmid
    #203799
    Purpose
    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. TFORF0973

    Plasmid
    #143252
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    ZNF124 (ZNF124 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  20. TFORF0459

    Plasmid
    #142266
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Insert
    CREB5 (CREB5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  21. p416-GPD-DIPP1-HA

    Plasmid
    #183946
    Purpose
    Expresses human HA-tagged DIPP1 from yeast GPD promoter
    Depositor
    Insert
    DIPP1 (NUDT3 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. Talin-TS

    Plasmid
    #83376
    Purpose
    FRET probe for measuring tension on Talin molecule
    Depositor
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted between V447 an…
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2101 - 2150 of 159111 results