4,114 results
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pBABE puro cyclinD1 HA
Plasmid#9050DepositorInsertcyclin D1 (CCND1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
UseTagshisExpressionBacterialMutationPromoterT7Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro mouse IRS-1 myc
Plasmid#11374DepositorInsertIRS-1 (Irs1 Mouse)
UseRetroviralTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-RabiesG
Plasmid#15785DepositorInsertRabies virus G protein (RABVgp4 Rabies virus (strain SAD B19))
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
mEos4b-N1
Plasmid#54814PurposeLocalization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneUseTagsmEos4bExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST51-LRRK2-G2019S
Plasmid#29401DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationGly2019SerPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65
Plasmid#68837PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for detailsDepositorInsertAncestral AAV Capsid Anc80L65
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterRep2Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lox-Stop-Lox TOPO
Plasmid#11584PurposeCre-dependent activation of gene of interest by removing Stop sequences; Puro selection.DepositorInsertloxP-STOP-loxP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150 - pDESTttTi5605[R4-R3]
Plasmid#19329DepositorArticleInsertpDESTttTi5605[R4-R3]
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro
Plasmid#141379PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPD95_75
Plasmid#1494DepositorTypeEmpty backboneUseTagsIVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1b
Plasmid#68811PurposeDoxycycline inducible lentiviral vector for FOXM1b expression in mammalian cellsDepositorInsertFOXM1b (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTOM20-ddGFP-B
Plasmid#40291DepositorInsertTOMM20 (TOMM20 Human)
UseTagsddGFP-BExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47 PINK1 C-GFP
Plasmid#13316DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor WT
Plasmid#12150DepositorInsertRhoA Biosensor (RHOA Human)
UseTags6xHisExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH-Myc-YAP-5SA
Plasmid#33093DepositorInsertYAP (YAP1 Human)
UseRetroviralTagsMycExpressionMammalianMutationS61A, S109A, S127A, S164A, S381A (phosphorylation…PromoterCMV/MSVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
YAP1 (S6A) - V5 in pLX304
Plasmid#42562DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationS61A, S109A, S127A, S128A, S131A, S163A, S164A, S…PromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cdk5GFP
Plasmid#1346DepositorInsertCDK5 (CDK5 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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Flag-HA-USP36
Plasmid#22579DepositorInsertUSP36 (USP36 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-targeted FRB (LDR)
Plasmid#20147DepositorInsertLyn-linker-FRB (LDR) (MTOR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO Hygro empty (w214-1)
Plasmid#17484Purpose3rd gen lentiviral empty control vector, CMV/TO promoter, HygroDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BP1-2 pLPC-Puro
Plasmid#19835DepositorInsertFragment of p53-Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsmCherryExpressionMutationFragment of human 53BP1 aa 1220 - 1711PromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFPV-mCherry
Plasmid#20956DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-HDM2
Plasmid#20935DepositorInsertMdm2 p53 binding protein homolog (MDM2 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pHR-CMV-nlsCRE
Plasmid#12265DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA
Plasmid#63931PurposeEmpty AAV-RAM plasmid w. multiple cloning site (MCS)DepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterpRAM and TREAvailable sinceDec. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(T17N)
Plasmid#13721DepositorInsertEGFP-Rac1(T17N) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV PRDM16
Plasmid#15504DepositorInsertPRDM16 (isoform 4) (Prdm16 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotermU6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pyronic/pcDNA3.1(-)
Plasmid#51308PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.DepositorInsertPyronic
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG WT
Plasmid#23298DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-N1
Plasmid#54493PurposeLocalization: N1 Cloning Vector, Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-C1-CAMKIIbeta
Plasmid#21227DepositorInsertCAMKIIbeta (Camk2b Rat)
UseTagsGFPExpressionMammalianMutationLab plasmid KS 0002.PromoterAvailable sinceJuly 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D1ER
Plasmid#36325DepositorInsertD1ER second generation cameleon (calcium sensor) targeted to ER
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flpo
Plasmid#55637PurposeExpresses Flpo in Mammalian CellsDepositorHas ServiceAAV Retrograde, AAV1, and AAV2InsertFlpo
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-Stx17 WT
Plasmid#45909DepositorInsertSyntaxin 17 (STX17 Human)
UseRetroviralTagsGFPExpressionMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
nls-NgAgo-GK
Plasmid#78253PurposeMammalian expression of NgAgo (Argonaute from N. gregoryi).DepositorInsertNgAgo
UseTagsa nuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA-EGFP
Plasmid#39862DepositorInsertmonomeric streptavidin fused to EGFP
UseTags6xHis, EGFP, and FLAGExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pGH224_sgRNA_2xMS2_Puro
Plasmid#85413PurposehU6 driven sgRNA vector with 2xMS2 vectors with puro selectable markerDepositorInsertpuromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEXTK-Pak1 70-117
Plasmid#12217DepositorInsertPak1 (PAK1 Human)
UseTagsGSTExpressionBacterialMutationPBD (p21-binding domain), aa 70-117PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
