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Showing: 7151 - 7200 of 160228 results

  1. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)

    Viral Prep
    #100853-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. pcDNA-RLuc8

    Plasmid
    #87121
    Purpose
    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
    Depositor
    Sanjiv Sam Gambhir
    Article
    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…
    Promoter
    CMV
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87121-plasmid-map-sequence-id-175252

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  7. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid
    #105714
    Purpose
    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
    Depositor
    Rylan Larsen
    Has Service
    AAV8
    Insert
    GCaMP6s
    Use
    AAV; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105714-plasmid-map-sequence-id-207265

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  8. CD Ugi3

    Plasmid
    #177195
    Purpose
    Greengate entry vector pGGC000 modified to contain bladderwort insulator 3
    Depositor
    Wayne Parrott
    Article
    Park et al bioRxiv 2021.11.04.467280
    Insert
    Utricularia gibba insulator 3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    177195-plasmid-map-sequence-id-361535

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  13. OTUB1

    Plasmid
    #25149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    otub1.040.271 (OTUB1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acid residues 40-271
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pSB700-mouse-ACTC1-Puro

    Plasmid
    #128326
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse ACTC1 for activation (Actc1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128326-plasmid-map-sequence-id-255029

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  15. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid
    #124537
    Purpose
    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.
    Depositor
    Tudor Fulga
    Article
    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:
    Insert
    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    d11-41 C55G, d11-41 C55G
    Promoter
    Minimal ADE
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124537-plasmid-map-sequence-id-242309

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  19. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    128325-plasmid-map-sequence-id-255042

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  20. pHR_TRE3G_EGFP_WPRE

    Plasmid
    #231954
    Purpose
    Lentiviral vector for inducible expression of the green fluorescent protein EGFP (TRE3G promoter)
    Depositor
    Leonardo Morsut
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231954-plasmid-map-sequence-id-470258

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  21. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  22. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid
    #223113
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223113-plasmid-map-sequence-id-447636

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  25. pcDNA3.1-hSyncytin-1

    Plasmid
    #182440
    Purpose
    hSyncytin-1
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182440-plasmid-map-sequence-id-357693

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  28. THINGL pcDNA3.1

    Plasmid
    #230046
    Purpose
    Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230046-plasmid-map-sequence-id-458381

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  29. THINGL-RR pcDNA3.1

    Plasmid
    #230047
    Purpose
    Expressing the human version of mScarletI-T2A-TINGL biosensor for glucose (named THINGL-RR), ratiometric variant with mScarletI.
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL-RR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230047-plasmid-map-sequence-id-458390

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  34. TFORF1386

    Plasmid
    #142387
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF362 (ZNF362 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142387-plasmid-map-sequence-id-427330

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  36. pSUSL001T:pTRE3G-dCas9-2xKRAB-p2a-tdTomato

    Plasmid
    #209298
    Purpose
    A lentiviral vector with tetracycline-inducible system to control expression of S. aureus dCas9 (with tdTomato) to silencing specific genes due to interference with gene transcription machinery.
    Depositor
    Birgitt Schuele
    Article
    Sastre et al Sci Rep. 2023 Oct 18;13(1):17796. doi: 1
    Inserts
    dCas9
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-inducible promoter (TRE3G)
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    209298-plasmid-map-sequence-id-414164

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  37. pCD-Ugi22

    Plasmid
    #207004
    Purpose
    pGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)
    Depositor
    Wayne Parrott
    Article
    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.
    Insert
    Utricularia gibba insulator 22
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207004-plasmid-map-sequence-id-411165

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  38. pCD-Ugi21

    Plasmid
    #207005
    Purpose
    pGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)
    Depositor
    Wayne Parrott
    Article
    Laspisa et al Front Plant Sci. 2023 Nov 8;14:1279231.
    Insert
    Utricularia gibba insulator 21
    Use
    Golden gate compatible cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207005-plasmid-map-sequence-id-411167

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  39. TFORF1971

    Plasmid
    #144318
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    YY2 (YY2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144318-plasmid-map-sequence-id-404164

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  43. Dox nontargeting shRNA GFP

    Plasmid
    #155290
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155290-plasmid-map-sequence-id-311906

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  46. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Mutation
    Promoter
    pTRE bidirectional tet-on
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  48. TFORF0490

    Plasmid
    #143030
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZSCAN4 (ZSCAN4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143030-plasmid-map-sequence-id-399059

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Showing: 7151 - 7200 of 160228 results