Skip to main content
Addgene
Showing: 851 - 900 of 19949 results

  2. pLenti CMV GFP Hygro (656-4) (Lentiviral Prep)

    Viral Prep
    #17446-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from pLenti CMV GFP Hygro (656-4) (#17446). In addition to the viral particles, you will also receive purified pLenti CMV GFP Hygro (656-4) plasmid DNA. Lentiviral particles carrying the GFP and hygromycin resistance.
    Depositor
    Eric Campeau, Paul Kaufman
    Article
    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.
    Promoter
    Tags
    None
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV9)

    Viral Prep
    #100854-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

    Enlarge

  14. pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)

    Viral Prep
    #178331-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Promoter
    Tags
    None
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Article
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119816-plasmid-map-sequence-id-372950

    Enlarge

  16. pAAV-CaMKIIa-hM4D(Gi)-mCherry (AAV5)

    Viral Prep
    #50477-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hM4D(Gi)-mCherry (#50477). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM4D(Gi)-mCherry plasmid DNA. CaMKIIa-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pSBtet-Pur

    Plasmid
    #60507
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60507-plasmid-map-sequence-id-322901

    Enlarge

  21. pGP-AAV-syn-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162376-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral Prep
    #62723-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato (Cre-dependent)
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-GFAP-hM4D(Gi)-mCherry (AAV5)

    Viral Prep
    #50479-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-hM4D(Gi)-mCherry (#50479). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM4D(Gi)-mCherry plasmid DNA. GFAP-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCherry
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. phCMV-coGALVenv-C4070A

    Plasmid
    #172217
    Purpose
    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors
    Depositor
    Boris Fehse
    Article
    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814
    Insert
    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Carries C-terminus of amphotropic MLV strain 4070…
    Promoter
    CMV immediate early promoter
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    172217-plasmid-map-sequence-id-341502

    Enlarge

  29. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

    Enlarge

  31. pAAV-hSyn-EGFP (AAV Retrograde)

    Viral Prep
    #50465-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. Synapsin-driven EGFP-expression control. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only

  35. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

    Enlarge

  36. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid
    #138150
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138150-plasmid-map-sequence-id-350242

    Enlarge

  37. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (AAV9)

    Viral Prep
    #108912-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (#108912). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 plasmid DNA. CAG-driven, Cre-dependent ChroME-ST-P2A-H2B-mRuby3 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hillel Adesnik
    Article
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Promoter
    CAG
    Tags
    mRuby3 (Cre-dependent)
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  39. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

    Enlarge

  40. pAAV.TBG.PI.Null.bGH (AAV8)

    Viral Prep
    #105536-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.TBG.PI.Null.bGH (#105536). In addition to the viral particles, you will also receive purified pAAV.TBG.PI.Null.bGH plasmid DNA. AAV vector with TBG promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    TBG
    Tags
    None
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  43. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

    Enlarge

  49. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)

    Viral Prep
    #105558-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    None
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 19949 results