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Showing: 451 - 500 of 157134 results

  2. Donor-PIGA

    Plasmid
    #153530
    Purpose
    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    Use
    Donor plasmid
    Tags
    N/A
    Expression
    Mutation
    No mutation
    Promoter
    No promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    153530-plasmid-map-sequence-id-319287

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  5. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128034-plasmid-map-sequence-id-422971

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  8. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234451
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234451-plasmid-map-sequence-id-465732

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  11. pAAV-CAG-GFP

    Plasmid
    #37825
    Depositor
    Edward Boyden
    Has Service
    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1
    Insert
    GFP
    Use
    AAV; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Aug. 8, 2012
    Availability
    Academic Institutions and Nonprofits only
    37825-plasmid-map-sequence-id-448204

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  14. pBS-CMV-gagpol

    Plasmid
    #35614
    Depositor
    Patrick Salmon
    Insert
    MLV gag and pol genes
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2012
    Availability
    Academic Institutions and Nonprofits only
    35614-plasmid-map-sequence-id-339908

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  17. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  18. phCMV-coGALVenv-C4070A

    Plasmid
    #172217
    Purpose
    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors
    Depositor
    Boris Fehse
    Article
    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814
    Insert
    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Carries C-terminus of amphotropic MLV strain 4070…
    Promoter
    CMV immediate early promoter
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    172217-plasmid-map-sequence-id-341502

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  20. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  29. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

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  33. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  34. pUMVC

    Plasmid
    #8449
    Purpose
    Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2005
    Availability
    Academic Institutions and Nonprofits only
    8449-plasmid-map-sequence-id-272509

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  35. lenti_dCas9-KRAB-MeCP2

    Plasmid
    #122205
    Purpose
    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
    Depositor
    Andrea Califano
    Article
    Tan et al bioRxiv 2021.06.28.449297
    Insert
    dCas9-KRAB-MeCP2
    Use
    Lentiviral
    Tags
    KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 (D10A, H840A)
    Promoter
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122205-plasmid-map-sequence-id-377395

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  36. pAdTrack-CMV

    Plasmid
    #16405
    Purpose
    Shuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.
    Depositor
    Bert Vogelstein
    Article
    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2008
    Availability
    Academic Institutions and Nonprofits only
    16405-plasmid-map-sequence-id-190476

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  37. tet-pLKO-sgRNA-puro

    Plasmid
    #104321
    Purpose
    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.
    Depositor
    Nathanael Gray
    Article
    Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    104321-plasmid-map-sequence-id-323839

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  39. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid
    #26730
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26730-plasmid-map-sequence-id-334693

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  40. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-372914

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  41. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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Showing: 451 - 500 of 157134 results