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177,145 results

Showing: 451 - 500 of 177145 results

  1. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid
    #46912
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-VP64-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    3xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    46912-plasmid-map-sequence-id-66610

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  4. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Publication
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    14 Articles
    119816-plasmid-map-sequence-id-372950

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  9. pCMVβ-p300-myc

    Plasmid
    #30489
    Purpose
    Mammalian expression of human p300 with myc tag
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Tags
    Myc
    Expression
    Mammalian
    Available since
    Oct. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24 Articles
    30489-plasmid-map-sequence-id-244762

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  11. Cre Reporter

    Plasmid
    #62732
    Purpose
    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).
    Depositor
    Niels Geijsen
    Publication
    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1
    Insert
    LoxP-DsRED-STOP-LoxP-eGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    LoxP sites
    Expression
    Mammalian
    Promoter
    EF1-Alpha
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    62732-plasmid-map-sequence-id-204219

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  14. pCX-OKS-2A

    Plasmid
    #19771
    Purpose
    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2
    Depositor
    Shinya Yamanaka
    Publication
    Okita et al Science. 2008 Nov 7. 322(5903):949-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    Expression
    Mammalian
    Mutation
    Three genes are connected with the foot-and-mouth…
    Available since
    Jan. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    19771-plasmid-map-sequence-id-354143

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  17. pCMV-GCaMP5G

    Plasmid
    #31788
    Purpose
    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.
    Depositor
    Douglas Kim, Loren Looger, GENIE Project
    Publication
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCaMP5G
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45 Articles
    31788-plasmid-map-sequence-id-228108

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  23. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    124646-plasmid-map-sequence-id-435118

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  27. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    HA
    Expression
    Mammalian
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    128034-plasmid-map-sequence-id-422971

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  28. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Publication
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    97421-plasmid-map-sequence-id-466895

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  37. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-alpha and EF1-alpha
    Available since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107 Articles
    39196-plasmid-map-sequence-id-335121

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  39. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid
    #20938
    Purpose
    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    channelrhodopsin-2
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CMV
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21 Articles
    20938-plasmid-map-sequence-id-181022

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  44. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    73795-plasmid-map-sequence-id-181202

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Showing: 451 - 500 of 177145 results