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177,145 results

Showing: 501 - 550 of 177145 results

  9. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid
    #110824
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19 Articles
    110824-plasmid-map-sequence-id-324008

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  14. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    41732-plasmid-map-sequence-id-210671

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  18. mEGFP-N1

    Plasmid
    #54767
    Purpose
    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    43 Articles
    54767-plasmid-map-sequence-id-204493

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  21. GW1CMV-Perceval

    Plasmid
    #21737
    Purpose
    can be used to monitor the ATP:ADP ratio during live-cell imaging
    Depositor
    Gary Yellen
    Publication
    Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Perceval (fluorescent ATP:ADP sensor)
    Tags
    5'UTR
    Expression
    Mammalian
    Mutation
    improved Kozak sequence
    Available since
    Aug. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    21737-plasmid-map-sequence-id-219888

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  29. mCherry-Lifeact-7

    Plasmid
    #54491
    Purpose
    Localization: Actin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Lifeact
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    56 Articles
    54491-plasmid-map-sequence-id-227430

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  33. pSQT1601

    Plasmid
    #53369
    Purpose
    human codon optimized Csy4 and FokI-dCas9 expression plasmid
    Depositor
    Keith Joung
    Publication
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    53369-plasmid-map-sequence-id-127248

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  34. pAAV-hSyn-EGFP

    Plasmid
    #50465
    Purpose
    EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial size
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    222 Articles
    50465-plasmid-map-sequence-id-240907

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  35. pCMV-PEmax-P2A-GFP

    Plasmid
    #180020
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    180020-plasmid-map-sequence-id-352752

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  43. pEBFP2-Nuc

    Plasmid
    #14893
    Purpose
    Mammalian expression of EBFP2 blue fluorescent protein with NLS
    Depositor
    Robert Campbell
    Publication
    Ai et al Biochemistry. 2007 May 22;46(20):5904-10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEBFP2-Nuc
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    14893-plasmid-map-sequence-id-122872

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  47. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    61408-plasmid-map-sequence-id-333613

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Showing: 501 - 550 of 177145 results