Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,238 results

Showing: 501 - 550 of 159238 results

  3. pDRGFP

    Plasmid
    #26475
    Purpose
    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker
    Depositor
    Maria Jasin
    Article
    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.
    Insert
    DRGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26475-plasmid-map-sequence-id-432928

    Enlarge

  4. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid
    #193298
    Purpose
    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    193298-plasmid-map-sequence-id-379909

    Enlarge

  9. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

    Enlarge

  10. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    13425-plasmid-map-sequence-id-360003

    Enlarge

  11. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

    Enlarge

  13. pET21a-Streptavidin-Alive

    Plasmid
    #20860
    Purpose
    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Alive
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Wt gene
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20860-plasmid-map-sequence-id-194024

    Enlarge

  14. pcDNA3-mRFP

    Plasmid
    #13032
    Purpose
    Mammalian expression vector for cloning your gene fused to mRFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Insert
    Monomer Red Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    13032-plasmid-map-sequence-id-211651

    Enlarge

  19. pCMV-dR8.2 dvpr

    Plasmid
    #8455
    Purpose
    2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8455-plasmid-map-sequence-id-370240

    Enlarge

  22. pAAV-hSyn-mCherry

    Plasmid
    #114472
    Purpose
    mCherry Control
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114472-plasmid-map-sequence-id-220931

    Enlarge

  33. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Article
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127289-plasmid-map-sequence-id-308425

    Enlarge

  38. pSF3-ultraID

    Plasmid
    #172878
    Purpose
    expresses ultraID in mammalian cells with the tet-on or tet-off system
    Depositor
    Julien Béthune
    Article
    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1
    Insert
    ultraID
    Use
    Luciferase and Retroviral; To be used in a tet-on…
    Tags
    myc
    Expression
    Mammalian
    Mutation
    fragment ([aa2-171]) of aquifex aeolicus BirA wit…
    Promoter
    TRE-cmv
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172878-plasmid-map-sequence-id-340526

    Enlarge

  46. Flag-TurboID

    Plasmid
    #124646
    Purpose
    Expresses Flag-TurboID fusion for proximity labeling
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124646-plasmid-map-sequence-id-435118

    Enlarge

  49. mTagRFP-T-Lifeact-7

    Plasmid
    #54586
    Purpose
    Localization: Actin, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    LifeAct -mTagRFP-T
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54586-plasmid-map-sequence-id-176185

    Enlarge

Showing: 501 - 550 of 159238 results