161,407 results

1. pNCS BFP

   Plasmid

   #91757

   Purpose

   Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Blue Fluorescent Protein
   

   Use

   Tags

   Hexa-Histidine, Xpress epitope for detection with…

   Expression

   Bacterial

   Mutation

   GFP (Y66H)

   Promoter

   T7

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF1932

   Plasmid

   #142292

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   JUN (JUN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ14107 fwYellow-targeting sgRNA

   Plasmid

   #239456

   Purpose

   guide for fwYellow

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   guide targeting fwYellow
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ3533 eforRed

   Plasmid

   #239451

   Purpose

   express eforRed chromoprotein

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   eforRed
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ3534 fwYellow

   Plasmid

   #239452

   Purpose

   express fwYellow chromoprotein

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   fwYellow
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLQ14112 tyrosinase-targeting sgRNA

   Plasmid

   #239751

   Purpose

   guide for tyrosinase

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   guide targeting tyrosinase
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLQ13960 tyrosinase(Priestia megaterium)

   Plasmid

   #239454

   Purpose

   express tyrosinase protein and melanin

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   tyrosinase
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ14103 aeBlue-targeting_sgRNA

   Plasmid

   #239750

   Purpose

   guide for aeBlue

   Depositor

   Stanley Qi

   Article

   Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

   Insert

   guide targeting aeBlue
   

   Use

   CRISPR; Cell-free system using bacterial extract

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

   Plasmid

   #238243

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-2xKS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLminP_Luc2P_RE38

    Plasmid

    #90378

    Purpose

    RELB - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    MAF

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ClpP-his

    Plasmid

    #22144

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    ClpP-His6
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    C-terminal His tag

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpCas9-CBE6d-V106W

    Plasmid

    #215827

    Purpose

    Express CBE6d V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-8xHis-Ub

    Plasmid

    #107392

    Purpose

    Mammalian expression 8xHis-Ub

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    8 tandem repeats of 6xHis-Ub (See comments below)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9(BB)-2A-miRFP670

    Plasmid

    #91854

    Purpose

    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988

    Depositor

    Ralf Kuehn

    Inserts

    hSpCas9-2A-miRFP670
    BB-guide RNA
    

    Use

    CRISPR

    Tags

    2A-miRFP670 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh and U6

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMKO.1 puro p53 shRNA 1

    Plasmid

    #10671

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-CMV-Galpha-q ONE-GO

    Plasmid

    #189738

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-q ONE-GO
    GNAQ (Gnaq Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFUW-VenusFlag-hCD44

    Plasmid

    #211824

    Purpose

    Express CD44 in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    N-Terminal Venus and Flag tags

    Expression

    Mammalian

    Mutation

    isoform corresponds to UniProt ID P16070-1 with a…

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GPI-RpHLuorin2

    Plasmid

    #171721

    Purpose

    GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to GPI anchor
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p16sheLL

    Plasmid

    #37320

    Depositor

    John Schiller

    Article

    Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

    Insert

    HPV16L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EGFP-PIP5K1A

    Plasmid

    #202720

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    EGFP:PIP5K1A (PIP5K1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGW1-Myc-DJ1-WT

    Plasmid

    #29347

    Depositor

    Mark Cookson

    Article

    Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psiCHECK-2-XNS

    Plasmid

    #196655

    Purpose

    Promega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directions

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFJ90 - Pmyo-2::mCherry::unc-54utr

    Plasmid

    #19327

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pmyo-2::mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p006-GFP-strong

    Plasmid

    #108313

    Purpose

    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4026

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. eGFP-proα1(I)

    Plasmid

    #119843

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223_KRAS_p.G12C

    Plasmid

    #81667

    Purpose

    Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G12C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

    Plasmid

    #158988

    Purpose

    Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-KRAB
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-Ef1a-DIO_EGFP-GFE3

    Plasmid

    #79871

    Purpose

    For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-GFE3
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CS2 Flag-Smad2

    Plasmid

    #14042

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-TRE-mTurquoise2-WPRE

    Plasmid

    #104110

    Purpose

    AAV-mediated expression of mTurquoise2 under the TRE promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. shp53 pLKO.1 puro

    Plasmid

    #19119

    Purpose

    3rd gen lentiviral vector for knocking down p53 gene expression

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p53 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Lyn-tailed mCherry-SEpHluorin

    Plasmid

    #32002

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    Membrane-targeting sequence of Lyn kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pST39-pNL29

    Plasmid

    #91696

    Purpose

    Expresses Mega GVs in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

    Insert

    Mega GV gene cluster (from pNL29) in pST39 backbone
    

    Use

    Tags

    No tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFA6a-6xGLY-His-tag-kanMX6

    Plasmid

    #20763

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid

    #164576

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid

    #221566

    Purpose

    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature (2025).

    Insert

    OpenCRISPR-1 sgRNA 16nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH

    Plasmid

    #184463

    Purpose

    pENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.

    Depositor

    Rhonda Kineman

    Article

    Lau-Corona et al Endocrinology. 2022 Apr 9. pii: 6565757.

    Insert

    N-Flag-mSTAT5bCA (Stat5b Mouse)
    

    Use

    AAV

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Point mutation at base 1963 (A to C), which chang…

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEGFP-N1-MITF-A

    Plasmid

    #38132

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-A (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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