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Showing: 501 - 550 of 157134 results

  1. PB-TO-MYOD1-shOct4

    Plasmid
    #182309
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    shOct4
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182309-plasmid-map-sequence-id-359523

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  3. pSBtet-Pur

    Plasmid
    #60507
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60507-plasmid-map-sequence-id-322901

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  7. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  8. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208051-plasmid-map-sequence-id-418310

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  10. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  12. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Use
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  16. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  22. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  28. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

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  29. pET-19b_MMLV-RT

    Plasmid
    #153312
    Purpose
    Bacterial expression of MMLV reverse transcriptase
    Depositor
    Philippa Marrack, Drew Endy
    Insert
    MMLV Reverse Transcriptase
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153312-plasmid-map-sequence-id-298742

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  34. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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Showing: 501 - 550 of 157134 results