166,607 results

1. pPD0039_CROP-seq-CAR-Puro

   Plasmid

   #242532

   Purpose

   CAR T screening

   Depositor

   Christoph Bock, Paul Datlinger

   Article

   Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

   Insert

   CD19-BB CAR (CD19 Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPB-cytERM-SUNTAG-MS2

   Plasmid

   #247298

   Purpose

   Mammalian expression of an ER-specific SUNTAG reporter; piggyBac vector to express cytERM-SUNTAG MS2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

   Insert

   ytERM-SUNTAG-MS2
   

   Expression

   Mammalian

   Available Since

   Nov. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR_PGK 3XFlag_4D5-5_CAR-mCherry

   Plasmid

   #247508

   Purpose

   Expresses a CAR against HER2 and mCherry

   Depositor

   Wendell Lim

   Article

   Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

   Insert

   CAR against Her2 and mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDH-CMV-HA-ORF-T2A-Puro

   Plasmid

   #242698

   Purpose

   gene expression

   Depositor

   Xingguo Zhu

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKOS6b-KOpstB

   Plasmid

   #237501

   Purpose

   Knockout of pstB

   Depositor

   Buz Barstow

   Insert

   Phosphate ABC transporter ATP binding subunit
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKOS6b-KOpstC

   Plasmid

   #237502

   Purpose

   Knockout of pstC

   Depositor

   Buz Barstow

   Insert

   Phosphate ABC transporter ATP binding subunit pstC
   

   Expression

   Bacterial

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKOS6b-URP114

   Plasmid

   #237505

   Purpose

   Upregulation of mgdh with P114

   Depositor

   Buz Barstow

   Insert

   membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
   

   Expression

   Bacterial

   Mutation

   Upregulation of mgdh with P114

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKOS6b-URtufB

   Plasmid

   #237506

   Purpose

   Upregulation of mgdh with tufB

   Depositor

   Buz Barstow

   Insert

   membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family
   

   Expression

   Bacterial

   Mutation

   Upregulation of mgdh with tufB

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NanoLuc-p53 Fusion Vector

   Plasmid

   #238870

   Purpose

   Express NanoLuc-p53 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   TP53 (TP53 Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. p53-NanoLuc Fusion Vector

    Plasmid

    #238871

    Purpose

    Express p53-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    TP53 (TP53 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. HaloTag-MDM2 Vector

    Plasmid

    #238678

    Purpose

    Express HaloTag-MDM2 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. HaloTag-p53 Vector

    Plasmid

    #238655

    Purpose

    Express HaloTag-p53 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    p53 (TP53 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. p53-HaloTag Vector

    Plasmid

    #238656

    Purpose

    Express p53-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    p53 (TP53 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. MSCV-KRAS-G12V-IRES-mCherry

    Plasmid

    #221025

    Purpose

    Fluorescent reporter for expressing the KRAS G12V mutant CDS

    Depositor

    Mohamed Fareh

    Article

    Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

    Insert

    KRAS G12V (KRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNL [NlucP/IFNb/Hygro] Vector

    Plasmid

    #236928

    Purpose

    Express IFNb in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    IFNb (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Available Since

    Aug. 12, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. NanoLuc-MDM2 Fusion Vector

    Plasmid

    #237198

    Purpose

    Express NanoLuc(R)-MDM2 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pNL [NlucP/HSE/Hygro] Vector

    Plasmid

    #236926

    Purpose

    Express HSE in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    HSE (HSD17B6 Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pNL [NlucP/MRE/Hygro] Vector

    Plasmid

    #236919

    Purpose

    Express MRE in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MRE
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNL [NlucP/ARE/Hygro] Vector

    Plasmid

    #236818

    Purpose

    Express ARE response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ARE
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. CL20_MSCV-NUP98::LNP1-ires-GFP

    Plasmid

    #237494

    Purpose

    Express NUP98::LNP1 in bicistronic retroviral vector

    Depositor

    Charles Mullighan

    Article

    Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

    Insert

    NUP98::LNP1 (LNP1, NUP98 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Tg(Sox17::EMTB-3xGFP)

    Plasmid

    #221190

    Purpose

    To specifically label microtubules (MTs) in Kupffer's Vesicle (KV)

    Depositor

    Heidi Hehnly

    Article

    Wu et al Development. 2025 May 15;152(10):dev2046

    Insert

    Ensconsin Microtubule-Binding Protein (MAP7 Human)
    

    Use

    Zebrafish

    Tags

    3 EGFP

    Mutation

    Microtubule binding-domain of ensconsin (A.A. 18…

    Promoter

    Sox17

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLD-puro-Cc-SARS-CoV-2_Spike_WT-VA

    Plasmid

    #191216

    Purpose

    Lentiviral expression of SARS-CoV-2 Spike_WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

    Insert

    SPIKE_SARS2_WT (S SARS CoV-2)
    

    Use

    Lentiviral

    Tags

    VA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Rat SARE-Arc Min Promoter-Flag Arc-Arc Genomic 3'UTR

    Plasmid

    #233056

    Purpose

    To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

    Depositor

    Jonathan Ploski

    Insert

    Rat Arc (Arc Rat)
    

    Use

    AAV

    Tags

    Flag

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

    Plasmid

    #220221

    Purpose

    AAV-STARR-seq validation vector with GFP

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

    Plasmid

    #222107

    Purpose

    Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

    Depositor

    Jonathan Weissman

    Article

    She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

    Insert

    CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
    

    Use

    AAV and CRISPR

    Tags

    HA and P2A-mCherry

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid

    #226104

    Purpose

    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    COX8A (COX8A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    encodes only for mitochondrial targeting sequence…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT-C34-L

    Plasmid

    #223967

    Purpose

    Anti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRetroX-TRE3G-IRSp53 S269D

    Plasmid

    #221319

    Purpose

    Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 (Baiap2 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    S269D

    Promoter

    TRE3G

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-HKGT3_mCherry2-NLS-PEST_Puro

    Plasmid

    #213771

    Purpose

    Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.

    Depositor

    Gaetano Gargiulo

    Article

    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

    Insert

    HKGT3_mCherry2-NLS-PEST
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Plasmid

    #211692

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pminiTol2-HuC-NE2m

    Plasmid

    #208688

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafish

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    Cloning vector

    Promoter

    Huc

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMPRAduo:minP:GFP

    Plasmid

    #193739

    Purpose

    MPRAduo ORF (minP:GFP) donor

    Depositor

    Ryan Tewhey

    Article

    Mouri et al Cell Genom. 2022 Dec 16;3(1):100234. doi

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TFORF3097

    Plasmid

    #144573

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF76 (ZNF76 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pN1-TOMM20-Halo

    Plasmid

    #129348

    Purpose

    Halo Tag, Mitochondrial outer membrane localization

    Depositor

    Yasushi Okada

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    Halo (Promega)
    

    Expression

    Mammalian

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEPQD1CB0112

    Plasmid

    #177044

    Purpose

    MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geraniol synthase (CrGES) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEPQD0CM0818

    Plasmid

    #177020

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase; geranyl diphosphate synthase
    

    Use

    Synthetic Biology

    Mutation

    transit peptide removed

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

    Plasmid

    #127776

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    tfap2b (TFAP2B Chicken)
    

    Use

    Unspecified

    Tags

    Avi

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pALPS puro miR30-IRF5

    Plasmid

    #117157

    Purpose

    Target site TATTTCCCTGTCTCCTTGGCC

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pALPS puro miR30-IRF7

    Plasmid

    #117158

    Purpose

    Target site ATAAGGAAGCACTCGATGTCG

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pV238-vPE

    Plasmid

    #225258

    Purpose

    Mammalian expression of vPE

    Depositor

    Vikash Chauhan, Robert Langer, Phil Sharp

    Article

    Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

    Insert

    vPE
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    R221K K848A H982A N1317R

    Promoter

    CMV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Tet-pLKO-puro

    Plasmid

    #21915

    Purpose

    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

    Depositor

    Dmitri Wiederschain

    Article

    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. M50 Super 8x TOPFlash

    Plasmid

    #12456

    Purpose

    Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAF0033 Ef1a-Pa01-2a-EGFP

    Plasmid

    #193460

    Purpose

    Expression of Pa01 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCL-Eco

    Plasmid

    #12371

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Insert

    gag/pol/env
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRC0940 (circRNA entry vector)

    Plasmid

    #189967

    Purpose

    Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCW-Cas9-Blast

    Plasmid

    #83481

    Purpose

    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

    Depositor

    Mohan Babu

    Inserts

    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    Tight Tre and human phosphoglycerate kinase 1 pro…

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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