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163,354 results

Showing: 561 - 600 of 163354 results

  2. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  10. YARS_pNIC-CTB10H

    Plasmid
    #153059
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    YARS (YARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153059-plasmid-map-sequence-id-304479

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  11. pLV/eGFP-U6-sgRNA

    Plasmid
    #170544
    Purpose
    A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR
    Depositor
    Jacob Giehm Mikkelsen
    Article
    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    170544-plasmid-map-sequence-id-377687

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  15. pDONR221-SV2A_STOP

    Plasmid
    #161423
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SV2A (SV2A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161423-plasmid-map-sequence-id-355049

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  21. pLKO.1-blast shLuc

    Plasmid
    #110409
    Purpose
    shLuc (Target TTACGCTGAGTACTTCGA), silence LUC gene, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (RNA PolIII)
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110409-plasmid-map-sequence-id-254625

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  24. FKBP1AA-c001

    Plasmid
    #53671
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    FKBP1AA
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. mTagRFP-T-Tubulin-6

    Plasmid
    #58026
    Purpose
    Localization: Microtubules, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pCRIS

    Plasmid
    #120424
    Purpose
    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
    Depositor
    Tamás Fehér
    Article
    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:
    Insert
    CRISPR spacers targeting IS1, IS5, IS3, IS150
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120424-plasmid-map-sequence-id-233246

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  36. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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Showing: 561 - 600 of 163354 results