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4,202 results

Showing: 701 - 750 of 4202 results

  1. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  3. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  4. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available Since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

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  5. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Expression
    Mammalian
    Available Since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    10676-plasmid-map-sequence-id-139182

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  8. pBabe-Kras G12C

    Plasmid
    #58901
    Purpose
    Expresses human Kras with a G12C mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12C mutation
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58901-plasmid-map-sequence-id-242434

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  10. pcDNA3.1_FASN

    Plasmid
    #107138
    Purpose
    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Insert
    Fatty acid synthase (FASN Human)
    Tags
    His
    Expression
    Mammalian
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107138-plasmid-map-sequence-id-423159

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  12. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  13. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  17. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  20. pmCherry Paxillin

    Plasmid
    #50526
    Depositor
    Kenneth Yamada
    Insert
    PXN paxillin (PXN Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50526-plasmid-map-sequence-id-241928

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  36. eIF2a 2

    Plasmid
    #21808
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52A
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21808-plasmid-map-sequence-id-229779

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  37. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  40. pCMV delta R8.2

    Plasmid
    #12263
    Purpose
    First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.
    Depositor
    Didier Trono
    Insert
    HIV-1 GAG/POL, Tat and Rev
    Use
    Lentiviral; Packaging
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12263-plasmid-map-sequence-id-394974

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  47. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89180-plasmid-map-sequence-id-176308

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  50. eIF2a 3

    Plasmid
    #21809
    Depositor
    David Ron
    Insert
    eIF2a (EIF2S1 Human)
    Expression
    Mammalian
    Mutation
    S52D
    Available Since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    21809-plasmid-map-sequence-id-358561

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Showing: 701 - 750 of 4202 results