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174,963 results

Showing: 641 - 680 of 174963 results

  11. pSicoR

    Plasmid
    #11579
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    11579-plasmid-map-sequence-id-251845

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  18. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  19. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available Since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  23. pAAV-CAG-tdTomato (codon diversified)

    Plasmid
    #59462
    Purpose
    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV
    Expression
    Mammalian
    Mutation
    codon diversified
    Promoter
    CAG
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    59462-plasmid-map-sequence-id-423489

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  24. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

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  25. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  26. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  31. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

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  35. Matrix-roGFP

    Plasmid
    #49437
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    Matrix-roGFP2
    Use
    Adenoviral
    Tags
    Cytochrome c oxidase subunit IV mitochondrial tar…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49437-plasmid-map-sequence-id-257693

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Showing: 641 - 680 of 174963 results