4,132 results
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pCDH-hMALAT1
Plasmid#118580Purposeto overexpress human MALAT1 in mammalian cellsDepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Beta1-GFP in pHcgreen
Plasmid#69804PurposeExpresses Beta1-GFP integrin for normal cell transfectionDepositorInsertBeta1A integrin (ITGB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo largeTcDNA
Plasmid#1780PurposeRetroviral expression of Large T Antigen. Used to create immortalized cell lines.DepositorInsertlarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-dTAG-BSD (BRD4)
Plasmid#91795PurposePITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.DepositorInsertFKBP_F36V-2xHA-P2A-BSD
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromotorlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMSP2N2
Plasmid#29520DepositorInsertMSP2N2
UseTags7-His tagExpressionBacterialMutationtandem MSP; His-tag on N-terminus followed by spa…PromoterAvailable sinceMay 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody
Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
UseTagsHHHHHHExpressionBacterial and MammalianMutationPromoterT7Available sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato
Plasmid#44332DepositorHas ServiceAAV5InserttdTomato
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
px330-mcherry
Plasmid#98750PurposeCas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Akaluc (neo)
Plasmid#124701PurposeAka-Luciferase reporterDepositorInsertVenus-Akaluciferase
UseLentiviralTagsExpressionMutationPromoterhPGKAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV_LaG17_synNotch_TetRVP64
Plasmid#79128PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)DepositorInsertLaG17 GFP nanobody synNotch TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9m4-VP64
Plasmid#47319PurposeCas9m4 ActivatorDepositorInsertCas9m4-VP64
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Kras G12D
Plasmid#58902PurposeExpresses human Kras with a G12D MutationDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsHAExpressionMammalianMutationG12D mutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1alpha
Plasmid#17652DepositorInsertHP1 alpha (CBX5 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WT
Plasmid#111906PurposeExpress RNASEH1 in mammalian cell.DepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-c-Met
Plasmid#31784DepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT4/HB
Plasmid#108352PurposeOptimized Sleeping Beauty Transposon Vector with MCSDepositorTypeEmpty backboneUseTransposonTagsExpressionMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLentiCRISPR-E
Plasmid#78852PurposeIntroduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsCas9-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE
Plasmid#162374PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV
Plasmid#24828DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo
Plasmid#25735Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-mCherry
Plasmid#45147DepositorInsertLAMP1-mCherry (LAMP1 Human, Synthetic)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ai9
Plasmid#22799PurposeRosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.DepositorInsertCAG-Floxed tdTomato
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike
Plasmid#145032PurposeExpress SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S Synthetic)
UseTagsC9ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-TRE-dCas9-VPR
Plasmid#63800PurposeSP-dCas9-VPR with doxycycline-inducible expressionDepositorInsertdCas9-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsVPR (VP64-p65-Rta)ExpressionMammalianMutationPromoterTREAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-GW
Plasmid#61703PurposeGateway compatible Y2H vector - BDDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only
