165,706 results

1. IL6R PLAK1

   Plasmid

   #161518

   Purpose

   Expresses IL6 receptor (full length) in mammalian cells.

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   Interleukin 6 Receptor (IL6R Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #232929

   Purpose

   Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   OsTir1(F74G)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 74 to Glycine

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

   Plasmid

   #184464

   Purpose

   Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

   Depositor

   Jennifer Doudna

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

   Insert

   PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCV-N LMP1

   Plasmid

   #37962

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   LMP1 (LMP-1 H. Herpesvirus 4 (EBV))
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti NbALFA-mNeonGreen puro

   Plasmid

   #193973

   Purpose

   lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-mNeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-anti-PDL1(KN035) PAGER(TF)

   Plasmid

   #230003

   Purpose

   Expresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   IL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   V360A/R361A on KORD

   Promoter

   CMV

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EX-HA-FRB-Cb5_pLX304

   Plasmid

   #120915

   Purpose

   Lentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   EX-HA-FRB-Cb5
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-OPA1

   Plasmid

   #26047

   Depositor

   David Chan

   Article

   Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

   Insert

   OPA1 (OPA1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. 2X PRE TK luc

    Plasmid

    #11350

    Depositor

    Donald McDonnell

    Article

    Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

    Insert

    PRE
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gCBEv3

    Plasmid

    #220618

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-myc-his-BACH1 WT

    Plasmid

    #17642

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OPA1-isoform1

    Plasmid

    #70173

    Purpose

    Mammalian expression of hsOPA1 isoform 1

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 1 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CRISPRoff-mScarletI

    Plasmid

    #217783

    Purpose

    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEP4 E02S EM2K

    Plasmid

    #20923

    Purpose

    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Oct4 and Sox2; Myc and Klf4 (SOX2, KLF4, MYC, POU5F1 Human)
    

    Use

    Episomal/ebna

    Available Since

    May 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ECFP HO

    Plasmid

    #207413

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ECFP CD

    Plasmid

    #207414

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

    Plasmid

    #107307

    Purpose

    Encoding an SPH activation system

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
    

    Expression

    Mammalian

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-GFP-Dub3 mouse

    Plasmid

    #72683

    Purpose

    mammalian expression of GFP-Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-mCherry

    Plasmid

    #180646

    Purpose

    Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX307-FURIN-puro

    Plasmid

    #158460

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TRPM4

    Plasmid

    #25903

    Depositor

    Craig Montell

    Article

    Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

    Insert

    TRPM4 (TRPM4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PKD1-F (pCI-PKD1-Flag)

    Plasmid

    #21369

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pECE-M2-PAK2 wt

    Plasmid

    #31663

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSum-LacZ-gfp

    Plasmid

    #109285

    Purpose

    Expresses gfp in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    GFP
    

    Expression

    Bacterial

    Mutation

    GFP modified for expression in prokaryotes; inclu…

    Promoter

    LacZalpha

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HSPB1

    Plasmid

    #155670

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    HSPB1 (HSPB1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

    Plasmid

    #113862

    Purpose

    Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CMV-eGFP
    TOP-NLuc1.1
    12GLI-Fluc
    CBF-GLuc
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIB166

    Plasmid

    #90189

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Tags

    None

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. qTAG-C-mScarlet-Blast

    Plasmid

    #207711

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. Fibronectin-human-EDB in pMAX

    Plasmid

    #120403

    Purpose

    enables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequence

    Depositor

    Inaam Nakchbandi

    Article

    Kraft et al J Mol Med (Berl). 2016 May;94(5):567-81.

    Insert

    EDB-fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJWV102-PL-dCas9

    Plasmid

    #85588

    Purpose

    Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    dCas9sp
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PLsp

    Available Since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJRH-1365 LV EF1a-CD28 IRES-EGFP

    Plasmid

    #201921

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD28

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge