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Mammalian Expression

76,286 results

Showing: 5801 - 5850 of 76286 results

  2. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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  7. pD649-HAsp-SIGLEC10-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156906
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC10 (SIGLEC10 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156906-plasmid-map-sequence-id-302921

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  8. GLuc-PM

    Plasmid
    #164783
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Gaussia princeps)
    Use
    Tags
    GLuc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164783-plasmid-map-sequence-id-326675

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  19. CMV-ER-LAR-GECO1

    Plasmid
    #61244
    Purpose
    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells
    Depositor
    Robert Campbell
    Article
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Insert
    LAR-GECO1
    Use
    Tags
    ER-retention sequence: KDEL and ER-targeting sequ…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1: V51W/I113V/N35…
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61244-plasmid-map-sequence-id-117077

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  20. HA-HA-AR-NTD-DBD

    Plasmid
    #171236
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171236-plasmid-map-sequence-id-336032

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  23. pCMV-SUN1 GFP

    Plasmid
    #187682
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Insert
    SUN1 (Sun1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    187682-plasmid-map-sequence-id-371222

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  29. p50 cFlag pcDNA3

    Plasmid
    #20018
    Depositor
    Stephen Smale
    Insert
    Nfkb1 (Nfkb1 Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p105 ligation site. It codes for…
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20018-plasmid-map-sequence-id-169435

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  34. pCMV6-XL4 FLAG-SFRP2-Fc

    Plasmid
    #115772
    Purpose
    Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    SFRP2 (SFRP2 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115772-plasmid-map-sequence-id-223829

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  35. pBABE

    Plasmid
    #21836
    Depositor
    David Ron
    Insert
    EGFP
    Use
    Retroviral
    Tags
    farnysylation signal
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    21836-plasmid-map-sequence-id-10029

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  40. pSLIK_hygro_3xflag_NRF2(RR)

    Plasmid
    #136535
    Purpose
    Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
    Depositor
    Kevin Janes
    Article
    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13
    Insert
    NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    This plasmid is developed by mutating 3 base pair…
    Promoter
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    136535-plasmid-map-sequence-id-304676

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  41. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  44. CXCR3-DuET

    Plasmid
    #213220
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR3 (CXCR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213220-plasmid-map-sequence-id-430782

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  49. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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Showing: 5801 - 5850 of 76286 results