Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

50+ requests Clear filter

Applied Filters

50+ requests Clear filter

9,047 results

Showing: 5301 - 5350 of 9047 results

  8. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Jesse Rinehart
    Insert
    sfGFP
    Expression
    Bacterial
    Promoter
    tetR/pLtetO
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52053-plasmid-map-sequence-id-442992

    Enlarge

  23. RHCglo

    Plasmid
    #80169
    Purpose
    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
    Depositor
    Thomas Cooper
    Article
    Singh et al Biotechniques. 2006 Aug;41(2):177-81.
    Insert
    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80169-plasmid-map-sequence-id-150808

    Enlarge

  33. pSicoR-Ef1a-mCh-Puro

    Plasmid
    #31845
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    U6 for shRNA; Ef1alpha for mCherry-puromycin
    Available Since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31845-plasmid-map-sequence-id-194345

    Enlarge

  38. pET15-MHL

    Plasmid
    #26092
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26092-plasmid-map-sequence-id-466943

    Enlarge

  40. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    nEF
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

    Enlarge

  44. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Article
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    106377-plasmid-map-sequence-id-205135

    Enlarge

Showing: 5301 - 5350 of 9047 results