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8,883 results

Showing: 5301 - 5350 of 8883 results

  14. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Article
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    89985-plasmid-map-sequence-id-180638

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  18. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

    Plasmid
    #84446
    Purpose
    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV1, AAV5, and AAV8
    Insert
    Jaws-KGC-tdTomato-ER2
    Use
    AAV
    Tags
    ER2, KGC, and tdTomato
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    84446-plasmid-map-sequence-id-317050

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  25. Pet28a_Synthetic_Human_H2A.1

    Plasmid
    #42634
    Depositor
    Joe Landry
    Insert
    Human H2A.1 (H2AC13 Synthetic, Human)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42634-plasmid-map-sequence-id-57749

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  31. CMV-Sp1

    Plasmid
    #12097
    Depositor
    Robert Tjian
    Insert
    Sp1 (SP1 Human)
    Expression
    Mammalian
    Mutation
    aa1-87 deleted
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    12097-plasmid-map-sequence-id-166326

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  34. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

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  35. pCAG HRP-TM

    Plasmid
    #44441
    Depositor
    Alice Ting
    Article
    Rhee et al Science. 2013 Jan 31.
    Insert
    Horseradish peroxidase fused to the transmembrane domain of PDGFR
    Tags
    HA tag and myc tag
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44441-plasmid-map-sequence-id-337239

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  38. mEmerald-FilaminA-N-9

    Plasmid
    #54098
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54098-plasmid-map-sequence-id-491758

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  42. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  45. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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Showing: 5301 - 5350 of 8883 results