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177,145 results

Showing: 901 - 950 of 177145 results

  11. pRGEB31

    Plasmid
    #51295
    Purpose
    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.
    Depositor
    Yinong Yang
    Publication
    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 and dual 35S promoter
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    51295-plasmid-map-sequence-id-339890

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  22. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    61358-plasmid-map-sequence-id-99789

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  23. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    50590-plasmid-map-sequence-id-210724

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  25. pcDNA3.1(+)/Luc2=tdT

    Plasmid
    #32904
    Purpose
    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
    Depositor
    Christopher Contag
    Publication
    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    Tags
    tdTomato red fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV-IE and n/a
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    26 Articles
    32904-plasmid-map-sequence-id-229770

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  34. pCMV14-3X-Flag-SARS-CoV-2 S

    Plasmid
    #145780
    Purpose
    Over expression of SARS-CoV-2 S (spike) in mammalian cell line
    Depositor
    Zhaohui Qian
    Publication
    Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (S SARS CoV 2)
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Codon optimized and C-terminal 19 amino acids del…
    Promoter
    CMV
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    31 Articles
    145780-plasmid-map-sequence-id-354441

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  36. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    27 Articles
    106280-plasmid-map-sequence-id-512176

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  47. pAAV.CMV.PI.EGFP.WPRE.bGH

    Plasmid
    #105530
    Purpose
    AAV expression of EGFP from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV rh10
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61 Articles
    105530-plasmid-map-sequence-id-454420

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  48. pCMV-lyso-pHluorin

    Plasmid
    #70113
    Purpose
    Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification
    Depositor
    Christian Rosenmund, Benjamin Rost
    Publication
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
    Tags
    HA-Tag and pHluorin
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    70113-plasmid-map-sequence-id-323570

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Showing: 901 - 950 of 177145 results